Supplementary Material
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Supplementary tables ERJ-01507-2016_Supplement
Figure S1. A representative inspiratory and expiratory CT scan from a subject with asthma illustrating the low attenuating clusters a) <950 HU in inspiration, and b) <856 in expiration. ERJ-01507-2016_Figure_S1
Figure S2. Examples of endobronchial biopsy sections; a) anti-endothelium (EN4) staining in a section with high Chalkley count, b) EN4 staining in a section with low Chalkley count, c) negative isotype control, and d) an example of a vascular hot spot on a EN4 stained section with superimposed point array from a Chalkley graticule normally fixed to the microscope eye piece. In each hotspot, after both rotating the graticule and moving the section, the Chalkley count is obtained by counting the maximum number of dots touching stained vessels. ERJ-01507-2016_Figure_S2