Abstract
Background: The spread of multidrug-resistant (MDR) and extensively drug-resistant (XDR) strains of M. tuberculosis (MBT) has led surgery as a possible adjunctive treatment. Resectedlung tissue is a good material to diagnose verification and characteristic of infection agent.
Objectives: Evaluate effectiveness of microbiological and molecular-genetic methods for diagnostic M. tuberculosis and its biological characteristic in surgical material.
Material and methods: Total of 386 lung tissues samples were obtained from patients undergoing pulmonary resection at Ural Research Institute for Phthisiopulmonology. After resection tissue were dissected for microbiology and molecular-genetic investigation.
Results: Of 386 specimens 28,2% were microscopy positive, 12,7% had positive culture. DNA MBT were detected in 95,6% ceases and in 88,1% it was enough for drug mutation detection. A total of 251 (65%) isolates were genotyped: 82% belong to Beijing lineage, 15% – to non-Beijing. 3% of specimens had mix of genotype. Beijing isolates in 65,5% ceases were MDR, 6,3% - XDR while only 7,9% non-Beijing isolates were MDR and no XDR ceases. In 15,1% Beijing and 55,3% non-Beijing isolates drug resistant mutation were not detected.
Conclusion: The uses of molecular-genetic methods are more reliable for diagnostic MBT and its characteristic in surgical material. Beijing lineage is main cause of MDR and XDR tuberculosis in Ural region.
- Copyright ©the authors 2016