Abstract
Background: In patients with chronic obstructive pulmonary disease (COPD) bacteria present in the lower airways during exacerbations might remain in respiratory tract in the stable phase of COPD. Bacteria may also worse the asthma course. However it is not clear whether the same intracellular pathways are activated by LPS in PBMC of patients with severe asthma and COPD.
Material and methods: PBMC isolated from 6 patients with severe asthma, 6 COPD and 6 healthy subjects were stimulated with LPS. Expression of genes belonging to 3 groups :phospholipase A2, eicosanoid pathway and cytokines were studied using TLDA cards.
Results:LPS significantly induced expression of ANXA1 in asthmatics and COPD patients. PLA2G15 and PLA2R1 genes exhibited similar expression pattern as ANXA1. Also PLA2G4, PLA2G7 and PAFAH1B gene showed trend toward differential expression in all groups.LTA4H was up-regulated in asthmatics and down-regulated in other groups.CYSLTR1, CYSLTR2 and LTB4R2 showed tendency to be up-regulated in COPD. We observed trend that PTGS2 and LTC4S genes were increased in all three groups whereas PTGS1expression was increased in asthmatics.ALOX12 was noticed as a negatively regulated gene in asthmatics.PBMC from COPD patients displayed elevated expression of IL1RL1. All other cytokines show tendency to be up-regulated with highest RQ value for IL8 and IL1B.
Conclusions: Asthma and COPD may be characterized by different LPS-induced expression profile of selected phospholipases, transcription factors and eicosanoid enzymes.
Funded by Polish National Science Center grant No. N N402 516939.
- © 2014 ERS