Abstract
To study IL-8 and TNF-α levels in blood and sputum in COPD exacerbation in dependence on availability and character of infectional factor.
52 COPD exacerbation patients were examined, infectional nature of exacerbations was proved at 35 patients (first group), infectional factor was not found at 17 patients (second group). The quantitative bacteriological sputum research, definition diagnostic main IgG, IgM levels to Ch.pneumoniae, M.pneumoniae in serum by means of immuno-assay method, in sputum by means of PCR method were made. IL-8, TNF-α levels in sputum and serum were defined by means of hardphase immuno-assay method.
Cytokines levels in the sputum at COPD exacerbation in first group were high than in second group: TNF-α level was 143.1±71.9 pg/ml and 31.0±12.6 pg/ml, (p=0.018), IL-8 level was 1244.5±197.4 pg/ml and 1088.8±222.5 pg/ml, (p=0.021).
Differences of TNF-α level were both in blood and in sputum in dependence on infectional agent species: in sputum its level was high in H.influenzae exacerbation (290.8±54. pg/ml, p=0.017) as compared with S.pneumoniae (110.6±45.9 pg/ml), M.pneumoniae (85.1±37.1 pg/ml), Ch.pneumoniae (132.6±32.1 pg/ml). While in blood TNF-α level was high in M.pneumoniae (887.2±164.2 pg/ml, p=0.019) as compared with S.pneumoniae (102.2±32.1 pg/ml), H.influenzae (182.2±44.9 pg/ml) and Ch.pneumoniae (495.0±118.3 pg/ml).
Il-8 level was different only in blood. Its level was high in H.influenzae exacerbation (112.0±47. pg/ml. p=0.008) as compared with S.pneumoniae (42.0±13.1 pg/ml), M.pneumoniae (36.2±19.5 pg/ml) and Ch.pneumoniae (53.0±37.2 pg/ml).Proinflammatory cytokines (Il-8, TNF-α) levels in sputum and blood were high at infectional COPD exacerbation.
- © 2011 ERS