Figures
Additional Files
Supplementary figures
Files in this Data Supplement:
- Supplementary figure 1 - Effects of fasudil, Y-27632 and azaindole-1 on proliferation of pulmonary artery smooth muscle cells (PASMCs) isolated from healthy rats. The proliferation assays were performed as described in methods. Effects of various concentrations of (a) fasudil, (b) Y-27632 and (c) azaindole-1 on thymidine incorporation into primary PASMCs isolated from healthy rats (n=3) are shown. Data are presented as % of FCS control. Bars represent mean ± SEM.
- Supplementary figure 2 - Effects of fasudil, Y-27632 and azaindole-1 on pulmonary artery smooth muscle cell (PASMC) viability. The effects of various concentrations of fasudil, Y-27632 and azaindole-1 on viability of PASMCs isolated from healthy and MCT-injected rats were performed using MTT assay as described in methods. Absorbance measured at 560nm as an estimation of the number of surviving PASMCs from (a) healthy and (b) MCT-injected rats are shown (n=3) is shown. Each dot represents mean ± SEM. Comparison of each concentration of the ROCK inhibitors versus without inhibitors (i.e. 0 nM or only vehicle) was done with T-test.
- Supplementary figure 3 - Effect of azaindole-1 on body weight of animals in hypoxia and monocrotaline (MCT) induced pulmonary hypertension. Body weights (BW, g) of animals were measured upon completion of the experiment at day 35. Effects of azaindole-1 treatment on BW of (a) MCT-injected rats and (b) hypoxic mice are shown. Bars represent mean ± SEM. Nox � normoxia; Hox � hypoxia. ***p <_0.001. _--="_--" end="end" desc="desc" data_supplement_figure_3.jpg="data_supplement_figure_3.jpg">