Fig. 7— Effect of azithromycin on pro-inflammatory cytokine production by alveolar macrophages (AMs). Azithromycin (500–10,000 ng·mL-1) was added to AMs for 24 h. Cytokine levels in culture supernatant were measured by cytometric bead array. There is a significant decrease in tumour necrosis factor (TNF)-α in the presence of 1,000 and 10,000 ng·mL-1 azithromycin and interleukin (IL)-8 in the presence of 10,000 ng·mL-1 azithromycin. Data are presented as mean±sem of five separate experiments, in triplicate. ▒: control (no azithromycin); ▓: 500 ng·mL-1 azithromycin; ░: 1,000 ng·mL-1 azithromycin; □: 10,000 ng·mL-1 azithromycin. *: p≤0.05 versus control.