To the Editor:
We read with interest the manuscript of Piacentini et al. 1. This paper shows that exhaled breath temperature plateau and exhaled nitric oxide (NO) are correlated in children with asthma.
This finding is interesting considering that NO is a marker of inflammation and that exhaled breath temperature may also be used to assess inflammation in the airways in a noninvasive way. We previously showed a similar correlation between exhaled NO and breath temperature in adults 2. However, we measured the rate of exhaled breath temperature increase (Δe°T) rather than the peak expiratory temperature (PET) and the plateau temperature (PLET) measured in this study. The use of Δe°T as a parameter to characterise the exhaled breath temperature curve allowed us to show that asthmatic patients have higher Δe°T values compared to those in normal subjects, possibly reflecting airway inflammation and bronchial blood flow. Contrary to what was shown in the study by Piacentini et al. 1, we were not able to show any correlation between PLET and exhaled NO, and PLET was not significantly different in asthmatic compared to normal subjects, confirming that Δe°T is a better parameter to characterise the curve.
Furthermore, the authors failed to recognise that Δe°T and plateau are dependent on the exhalation flow rate 2. The children were asked to perform a “slow expiratory act” but the exhalation flow rate was not standardised, making the results unreliable. Moreover, the mouth pressure generated during exhalation should be determined to confirm that the soft palate was effectively closed avoiding contamination of the exhaled breath with nasal air.
In addition, the intersession and intrasession reproducibility of the method was not investigated.
The lack of standardisation of the method may explain some of the unexpected findings in this paper, such as the failure of steroids to decrease exhaled breath temperature despite their efficacy in reducing bronchial blood flow 3. Furthermore, exhaled NO levels were similar in steroid-treated and untreated children, whereas it is well established that steroids effectively reduce the levels of exhaled NO 4.
We feel that the methodological inexactitudes and the unexpected findings deserve a comment by the authors, which was not provided in the paper as published.
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