European Respiratory Society


The natural history of the development of sensitization and disease due to high-molecular-weight allergens is not well characterized. This study describes the time-course of the incidence of work-related symptoms, skin reactivity and occupational rhinoconjunctivitis (RC) and asthma (OA); and assesses the predictive value of skin testing and RC symptoms in apprentices exposed to laboratory animals, in a 3–4-yr programme.

Four-hundred and seventeen apprentices at five institutions were assessed prospectively with questionnaire, skin-testing with animal-derived allergens, spirometry and airway responsiveness (n=373). Depending on the school, students were seen 8 (n=136), 20 (n=345), 32 (n=355) and 44 (n=98) months after starting the programme.

At all visits, the incidence was greater for work-related RC symptoms followed in order by skin reactivity, occupational RC, and, almost equally, OA and work-related respiratory symptoms. The incidence-density figures were comparable for each follow-up period and for most indices up to 32 months after entry into the study and then tended to decrease. The positive predictive values (PPVs) of skin reactivity to work-related allergens for the development of work-related RC and respiratory symptoms were 30% and 9.0%, respectively, while the PPVs of work-related RC for the development of OA was 11.4%.

Sensitization, symptoms and diseases occur maximally in the first 2–3 yrs after starting exposure to laboratory animals. Skin reactivity to work-related allergens and rhinoconjuctivitis symptoms have low positive predictive values.

This study was supported by the Medical Research Council of Canada (grant no MT-12256) and the Fonds de la Recherche en Sante du Quebec.

Laboratory animals are a common cause of occupational asthma (OA) due to high-molecular-weight allergens 1. Rat urine is the major and most potent source of inhaled antigens 1. Although several cross-sectional studies have been carried out as summarized elsewhere 1, very few longitudinal studies have followed laboratory animal workers prospectively. Cullinan et al. 2 followed a cohort of 342 previously exposed laboratory animal workers on a 6-month basis over a 3.5-yr interval. They reported incidence rates of 7.3/100 person-yrs for eye/nose symptoms, 4.1/100 person-yrs for the development of skin reactivity to rat urinary allergens, 4.8/100 person-yrs for skin symptoms and 3.5/100 person-yrs for respiratory symptoms. A cohort of 769 apprentices starting exposure to high-molecular-weight antigens, including 417 in animal-health technology programmes has previously been described 3. In this specific programme, it was shown that 85 of the 394 participants 921.5% of persons at risk developed skin reactivity to laboratory-animal-derived allergens over the follow-up period, corresponding to 7.9/100 person-yrs 4. It was also found that the incidence of OA defined by the presence of skin reactivity to a programme-specific antigen and significant changes in bronchial responsiveness to methacholine was 2.7% (28/1043 person-yr) 5. The purpose of this analysis, carried out in the 394 participants in the prospective study of laboratory-animal workers, was to describe the time-course of incident cases of skin reactivity, work-related rhinoconjunctivitis (RC) and respiratory symptoms and OA over the 3–4-yr-duration of the programme. The hypothesis was that the incidence would be higher in the period immediately after starting exposure and diminish thereafter. The value of skin reactivity and work-related RC was also assessed as an early marker for the development of OA. Such an approach has been advocated in the prevention of OA 6 but has not been validated.

Subjects and methods

The subjects were 417 apprentices in animal-health technology, part of a cohort of 769 apprentices (including 230 apprentices in pastry-making and 122 in dental hygiene technology) recruited from 14 specialized schools (five in animal-health technology) upon starting their training between 1993 and 1995. Baseline characteristics of the cohort members have been reported elsewhere 3. Subjects in these schools were eligible for the cohort study provided that they had not been exposed to the relevant work-related aero-allergens in an apprenticeship or at work for at least three months before entering the career programme, as assessed during a preliminary visit. The duration of the animal-health technology programme was either 32 or 44 months. The majority of students (n=307) entered the 32-month duration programme while the rest (n=110) attended the longest programme, of 4-yrs duration. After the baseline assessment, the students were reassessed at 8, 20, 32 and 44 months after starting the programme. The majority of students attended the 20-month (n=345, participation rate=82.7%) and 32-month (n=355, participation rate=85.1%) visits. There were 136 students at the 8-month assessment because the others had not been exposed to laboratory animals since starting in the programme. Finally, 98/110 (89.0%) subjects in the 44-month duration programme attended the last visit.

The study was approved by the ethics committee of Sacré-Coeur Hospital. The subjects gave written consent to their participation when recruited in this cohort study.

At the time of entry into the apprenticeship programme and at each follow-up visit, each student answered a respiratory questionnaire derived from the standardized questionnaire of the International Union against Tuberculosis and Lung Diseases (IUATLD) 7. At each visit, nasal, ocular, respiratory, and skin symptoms were assessed during exposure to the specific agents present during work in the laboratory, allowing the work-relatedness of the symptomatology to be assessed.

Skin-tests were performed using the skin-prick method, using 11 common inhalants: mixed trees, mixed grass, and ragweed pollens; Alternaria, Aspergillus, Hormodendrum; feathers; Dermatophagodes farinae and D. pteronyssinus; and cat and dog dander (Omega, Montreal, Canada). Histamine phosphate (1/200 g·mL−1) was used as a positive control, and diluent (glycerine, 50%), as a negative control. The largest weal diameter was assessed 10–15 min after introducing the antigen. A positive reaction was defined as a weal ≥3 mm in the absence of reaction to the diluent and in the presence of a positive reaction to histamine phosphate.

In addition, skin-prick-tests were performed with extracts from aero-allergens potentially present in the working areas of students and confirmed as specific tothe programme by the authors 4. These consisted ofurinary proteins from rat, mouse and rabbit (Pharmacia Allergon AB, Angelholm, Sweden), as well as rabbit danders (Omega). The criterion for a positive reaction to a specific allergen was the same as for common allergens. Subjects who demonstrated sensitization to a work-related antigen at the initial visit were also followed to detect possible new sensitization to another occupational allergen.

All extracts were obtained before the beginning of the study in sufficient amounts to perform the estimated number of skin-tests to the end of the project. Each extract was prepared from the same batch. The same nurse performed the skin-prick-testing throughout the study.

Spirometry with the assessment of forced expiratory volume in one second (FEV1) and forced vital capacity (FVC) was carried out using a Collins apparatus (Survey/1 Plus; Collins, Braintree, MA, USA) according to published standards 8. Methacholine inhalation tests were performed using a Wright's nebulizer (Roxon Meditech Ltd, Montreal, Quebec, Canada) (output=0.14 mL·min−1) at tidal volume breathing for 2 min according to guidelines slightly modified from those of the European Respiratory Society 9. The procedure for performing the methacholine test was modified as described elsewhere to take into account the absence of an on-site physician 10. The provocative concentration causing a 20% fall in FEV1 (PC20) was interpolated from individual dose-response curves drawn on a semilogarithmic scale using noncumulative doses.

Reference values for FEV1 and FEV1/FVC were taken from Knudson et al. 11. Bronchial hyperresponsiveness (BHR) was set at ⪕16 mg·mL−1 12.

New sensitization was defined as changes in skin reactivity (from absent to present) occurring at one of the follow-up visits and remaining positive thereafter. New work-related symptomatology was defined in the same way. Incident cases of occupational RC were defined as subjects who developed a specific immunological sensitization to at least one work-related allergen and nasal and/or conjunctival symptoms after the baseline visit. Incident cases of OA were defined as subjects who developed a specific immunological sensitization to at least one work-related allergen and a 3.2-fold decrease in PC20 methacholine after the baseline visit 13.

Statistical analysis

The proportion of incident cases of skin reactivity, work-related symptoms, occupational RC and asthma relative to the number of subjects present at each visit was computed. The positive predictive values of skin reactivity at baseline or follow-up for subsequent and/or concomitant outcome were calculated. This estimate was obtained by summing up the new cases of symptoms occurring at subsequent visits and half of those detected at the same visit relative to the occurrence of skin reactivity. The assumption was made that when skin reactivity and work-related symptoms were detected concomitantly, skin reactivity preceded the development of symptoms in 50% of cases. The same rationale holds for occupational RC in relation to OA. Statistical analyses were performed using the Statistical package for the Social Sciences (SPSS) software (SPSS version 9.1 for Windows; SPSS, Chicago, IL, USA).


Table 1 shows the number of subjects with the outcomes that were examined and the percentages relative to those seen at each visit. At most visits, the incidence figures were greater for work-related RC symptoms followed in order by skin reactivity to programme-related antigen, work-related skin symptoms, occupational RC and, almost equally, OA and work-related respiratory symptoms. The incidence of programme-related antigen skin reactivity and RC symptoms was higher at 1–2 yrs after entering the programme, while it was higher at 2–3 yrs for respiratory symptoms and at 3 yrs for occupational RC. The percentages of new events were lower for alloutcomes in the fourth yr after entering the programme.

View this table:
Table 1—

Time-course of work-related symptoms and occupational diseases in apprentices exposed to laboratory animals

The percentage of new cases of OA were comparable in years 1–3 of the programme. The percentage of incident cases of sensitization to a programme-related antigen in the fourth yr of the programme (5.1%) was comparable to the incidence of sensitization to mites (4.6%) and pets (3.8%) during the study period. One-hundred and twenty-nine subjects had skin reactivity to a programme-related antigen at the end of follow-up, including 44 at baseline and 85 incident cases 4. Of the 85 incident cases of sensitization to an animal-derived antigen, 79 (93%) had methacholine inhalation testing at one or more follow-up visits. Of the 203 subjects who underwent methacholine testing at one or more follow-up visits, 53 (26.1%) dropped their PC20 values by at least a 3.2-fold difference. Of these 53, 28 (43%) were cases of OA and represent the burden of occupational exposure on all incident cases of asthma in this cohort. Four of the remaining 25 individuals were incident cases of sensitization to mites (n=2) or pets 3 (none to pollens or moulds). Therefore, 28 of the 373 subjects (7.5%) in the cohort satisfied the definition of OA.

Table 2 gives the number of incident cases of work-related symptoms and the timing of the occurrence of symptoms in relation to skin reactivity. The positive predictive values (PPVs) were higher for RC symptoms than for respiratory symptoms. It is estimated that 27 (i.e. 17+(19/2) in the nasal and/or ocular symptom line of table 2) of the 99 incident cases of work-related RC showed skin reactivity to a programme-related antigen before symptoms. Eleven (i.e. 9+(4/2) in the dyspnoea and/or wheezing line of table 2), of the 22 (50%) incident cases of work-related respiratory symptoms had skin reactivity to a programme-related antigen before the onset of symptoms. Nineteen of the 122 cases (15.6%) with skin reactivity who were at risk developed respiratory symptoms during the course of the study. The PPV of work-related RC symptoms for the development of OA was 11.4% while the PPV of work-related respiratory symptoms for the development of OA was 25%.

View this table:
Table 2—

Incidence of work-related symptoms in relation to the time of occurrence of skin reactivity to laboratory animal-derived antigens


In this study it was found that the peak incidences of skin reactivity and work-related RC symptoms are maximal 1–2 yrs after entering a programme, while occupational RC defined by the occurrence of both skin reactivity and work-related RC is at its peak 3 yrs after entering the programme. The number of cases of OA is comparable throughout the first 3 yrs.

There are few longitudinal studies of workers exposed to laboratory antigens and none in which subjects were followed from the beginning of exposure and assessed for symptoms, immunological reactivity and bronchial responsiveness. Results on the incidence and risk factors for immunological sensitization were recently reported for a cohort of 769 apprentices 4. Cullinan et al. 2 prospectively assessed 342 laboratory animal workers. In contrast to the present study subjects, these workers had up to 4 yrs of exposure to laboratory animals before the onset of the study and bronchial responsiveness was not assessed. The incidence-density in the present study for programme-related antigen skin reactivity (7.9 per 100 person-yrs) was twice that found by Cullinan et al. 2 (4.1 per 100 person-yrs). The incident-density of RC symptoms wasalso higher in the presented study: 12.0 per 100 person-yrs as compared with 7.3 per 100 person-yrs in the study of Cullinan et al. 2. It was found that the percentage of new cases of skin reactivity and RC symptoms were higher in the first 2 yrs of the programme; Cullinan et al. 2 reported similar findings in their cohort of workers after they started new employment. The higher incidence figures in the present study compared with those from the study by Cullinan et al. 2 could be explained by the fact that in the present study, the subjects were assessed earlier after starting exposure for the development of outcomes than in the British study, thus minimizing a potential survival bias. Furthermore, the apprentices studied here were exposed to three species of rodents, and therefore the potential for developing new work-related antigen skin reactivity was increased; indeed, if only incident skin reactivity to rat was considered, the incidence density would be 5.9 per 100 person-yrs. It is interesting to note that the incidence of sensitization in the fourth year of the programme (5.1%) is of the same order of magnitude as the incidence density of sensitization to common allergens (4.6% for mites and 3.8% for pets) throughout the study. Therefore, after a rise in the first 2 yrs in the programme, the incidence of sensitization decreases in the third yr and reaches the incidence of skin reactivity to common allergens after 4 yrs. This pattern has relevant significance in term of prevention. Indeed, if the decision to screen for sensitization is made, the relevant timing should be in the first 3 yrs of exposure. Theoretically, it is logical to assume that immunological reactivity to a work-related antigen would develop first, followed by the involvement of the target organ, i.e. the nose/eye and respiratory tract. From the present data, it appears that this represents the time-course model in the case of occupational RC, whose highest incidence occurred later than the highest incidence of work-related antigen skin reactivity. The theoretical chronological pattern was not found in the case of OA; this is probably difficult to interpret because there was not a sufficient number of cases. Also, shorter time interval between assessments of bronchial responsiveness may be indicated. The low PPVs of skin reactivity for the development of symptoms and diseases suggest that the theoretical model of the natural history of OA is not satisfactory. This suggests that chronological events may follow another pattern e.g. symptoms may precede the onset of skin reactivity. However, it must be mentioned that the method used to calculate the predictive value may have resulted in an underestimation. Indeed, it is hypothesized that skin reactivity preceded the development of symptoms in 50% of cases when skin reactivity and work-related symptoms were detected concomitantly.

It is unlikely that the described time-course of sensitization, symptoms and disease had been affected by an attrition bias. Only 36/417 subjects (8.6%) left the programme and work-related symptoms during training were not associated with quitting 14. Only two subjects, the two in the animal-health technology programme, reported leaving due to work-related RC symptoms. The proportion of apprentices who reported at least one of the skin, RC or chest symptoms was not different in quitters (7/27, 26%) and in those who stayed (94/370, 25%). It is also unlikely that the pattern of sensitization and symptoms could have been affected by an exposure pattern with enhanced exposure in the first 2 yrs of the programme. The students were indeed exposed to rodents, the main sensitizer 4 for, on average, 14 h in the first yr, 10 h in the second and 91 h in the third. There were also no changes in animal housing facilities during the course of the programme. Finally, PPVs were estimated. Since this study is a cohort the PPV is not biased by artificial prevalence as in case-control studies.

Secondary prevention of OA is based on the identification of cases at an early stage. For this, two tools have been considered. First, as immunological reactivity theoretically precedes the onset of OA with alatency period, assessment of skin reactivity was proposed 15. Second, RC symptoms generally precede the onset of OA 16 and can, therefore, be suggested in a prevention strategy. Specific sensitization is highly associated with the development of work-related symptoms (statistically significant relative risks (95% confidence intervals) of 6.8(4.2–11.1) for RC and 4.9(2.2–10.8) for respiratory symptoms (unpublished data)). Although it was found that skin reactivity had a low predictive value for work-related RC (30%) and respiratory symptoms (9%), the data also show that 50% of the 22 incident cases of work-related respiratory symptoms had skin reactivity to a programme-related antigen before the development of symptoms. Also, 22 of the 122 cases (18.0%) with skin reactivity who were at risk developed respiratory symptoms during the course of the study. Moreover, the predictive value of RC symptoms in regards to the development of respiratory symptoms was 30%, which is not negligible. Finally, a follow-up of 4 yrs is relatively short as incident cases of work-related symptoms may occur later. This being said, taken together, these data suggest that assessment of skin reactivity and RC symptoms should still be considered in the context of screening programmes. The use of skin reactivity as a predictor for symptoms and diseases and the use of RC symptoms as a predictor of the development of lower respiratory symptoms also needs to be assessed from the points of view of cost-effectiveness and compliance.

In conclusion, this description of the pattern of the natural history of sensitization, symptoms and diseases in apprentices starting exposure to laboratory animals, should be examined in more depth to confirm the temporal trends.


The authors express their gratitude to the technicians, principally J. L'Archeveque, and to nurse M. Magnan, who played a key role in the recruitment of subjects and the handling of results. They also thank all those responsible at the participating teaching institutions and all of the students who took part in the study. Finally, they acknowledge the collaboration of L. Schubert for reviewing the manuscript.

  • Received July 24, 2000.
  • Accepted December 12, 2000.


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