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Eur Respir J 2007, doi:10.1183/09031936.00117506
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ORIGINAL ARTICLE |
Effect of tuberculin skin testing on a Mycobacterium tuberculosis-specific IFN-
assay
1 Dept of Infectious Diseases
2 Heart Lung Center Utrecht and Dept of Pulmonology
3 Dept of Microbiology, Diakonessenhuis Utrecht/Zeist,Utrecht, the Netherlands
4 Dept of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands
* To whom correspondence should be addressed. E-mail: S.M.Arend{at}LUMC.nl.
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Abstract |
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Recently, interferon-gamma release assays (IGRA) for specific diagnosis of Mycobacterium tuberculosis infection have become available. In recent U.K. tuberculosis guidelines it is now advised to screen for latent M. tuberculosis infection using tuberculin skin testing (TST) followed by IGRA if the TST is positive. Since TST can boost immune responses to tuberculin, we evaluated whether TST administration affects the result of QuantiFERON®-TB Gold in-tube (QFT-GIT), a whole blood-based IGRA.
QFT-GIT was performed on the day of TST administration and the day of reading in 15 TST-negative persons, 46 TST-positive persons with recent or remote exposure to M. tuberculosis and 5 cured TB patients.
No systematic boosting of QFT-GIT responses from negative to positive was observed. Only in a few TST+ persons TST enhanced pre-existing QFT-GIT responses.
Screening for latent M. tuberculosis infection using TST followed by IGRA on the day of reading is a reliable approach as the specificity of QFT-GIT was not affected by prior TST administration.
Keywords: Diagnosis, IFN, immunoassays, Mycobacterium tuberculosis, skin test, tuberuclosis
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