|
 |
|
|||||||
|
|||||||||
Eur Respir J 2006, doi:10.1183/09031936.00111205
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ORIGINAL ARTICLE |
Lung and blood mononuclear cell responses of TB patients to mycobacterial proteins
1 Dept of Biochemistry
2 Dept of Pulmonary Medicine, Postgraduate Institute of Medical Education & Research, Chandigarh - 160 012 [India]
* To whom correspondence should be addressed. E-mail: gkkhuller{at}yahoo.co.in.
 |
Abstract |
|---|
The differences in specificity of human lung and peripheral lymphocytes for the mycobacterial antigens need to be evaluated to identify vaccine candidates against pulmonary tuberculosis (TB).
Therefore, responses to low molecular weight secretory proteins of Mycobacterium tuberculosis were examined in bronchoalveolar lavage (BAL) and peripheral blood mononuclear cells (PBMCs) from minimal pulmonary TB and non-TB patients.
Ag85A, Ag85B, CFP-31, CFP-22.5, CFP-21, MPT-64 and as yet uncharacterized 19 kDa protein were found to be predominantly recognized by BAL cells of TB patients on the basis of lymphocyte proliferation and significant IFN- 
release. However, recognition of CFP-8, ESAT-6, CFP-10, CFP-14.5, MTSP17 and five other as yet uncharacterized low molecular weight polypeptides was found to be high on the basis of lymphocyte proliferation at the level of PBMCs. Further, BAL macrophages and not blood monocytes were found to produce nitric oxide in response to mycobacterial antigens. Among polypeptides predominantly recognized by BAL lymphocytes, only Ag85A and B were found to induce both nitric oxide and IL-12 (p40) by alveolar macrophages.
These results indicate heterogeneity in antigen recognition by BAL cells and PBMCs of minimal TB patients and also suggest the utility of Ag85 complex polypeptides for the development of future mucosal antituberculous vaccine.
Keywords: Immune response, low molecular weight polypeptides, lung, peripheral blood, tuberculosis, vaccine
This article has been cited by other articles:
|
|
 |
|
  K Dheda, R N van Zyl-Smit, R Meldau, S Meldau, G Symons, H Khalfey, N Govender, V Rosu, L A Sechi, A Maredza, et al. Quantitative lung T cell responses aid the rapid diagnosis of pulmonary tuberculosis Thorax, October 1, 2009; 64(10): 847 - 853. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Oswald-Richter, D. A. Culver, C. Hawkins, R. Hajizadeh, S. Abraham, B. E. Shepherd, C. A. Jenkins, M. A. Judson, and W. P. Drake Cellular Responses to Mycobacterial Antigens Are Present in Bronchoalveolar Lavage Fluid Used in the Diagnosis of Sarcoidosis Infect. Immun., September 1, 2009; 77(9): 3740 - 3748. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. L. Beamer, D. K. Flaherty, B. Vesosky, and J. Turner Peripheral Blood Gamma Interferon Release Assays Predict Lung Responses and Mycobacterium tuberculosis Disease Outcome in Mice Clin. Vaccine Immunol., March 1, 2008; 15(3): 474 - 483. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Jafari, M. Ernst, A. Strassburg, U. Greinert, B. Kalsdorf, D. Kirsten, and C. Lange Local immunodiagnosis of pulmonary tuberculosis by enzyme-linked immunospot Eur. Respir. J., February 1, 2008; 31(2): 261 - 265. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. H Bothamley New tests for tuberculosis: local immune responses have greater specificity Thorax, January 1, 2008; 63(1): 4 - 5. [Full Text] [PDF]
|
|
|
|
|
|
Y. J. Ryu, E. J. Kim, S-H. Lee, S. Y. Kim, G. Y. Suh, M. P. Chung, H. Kim, O. J. Kwon, and W-J. Koh Impaired expression of Toll-like receptor 2 in nontuberculous mycobacterial lung disease Eur. Respir. J., October 1, 2007; 30(4): 736 - 742. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |