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Eur Respir J 2006, doi:10.1183/09031936.00084006
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ORIGINAL ARTICLE |
Exhaled breath condensate pH standardised for CO2 partial pressure
1 Dept of Pathophysiology and
2 Institute of Human Physiology and Clinical Experimental Research, Semmelweis University, Budapest, Hungary
3 Clinical Laboratory, National Korányi Institute for TB and Pulmonology, Budapest, Hungary
4 Dept of Pathophysiology and; and Institute of Human Physiology and Clinical Experimental Research, Semmelweis University, Budapest, Hungary
* To whom correspondence should be addressed. E-mail: kiss.horvath{at}t-online.hu.
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Abstract |
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Exhaled breath condensate pH is considered to reflect acid-base balance of the airways. Current pH measurements do not take into account the effect of CO2. The aim of this study was to determine the effect of condensate CO2 partial pressure on pH and to provide a more precise mode of EBC pH determination.
Condensate pH and CO2 partial pressure were measured in parallel from 12 healthy volunteers and 12 asthmatics by blood gas analyser in neat, argon deaerated and CO2 loaded samples. Regression analysis was used a) to test the relation between pH and CO2, b) to calculate pH at 5.33 kPa CO2 level (physiological alveolar CO2 partial pressure). Reproducibility of different pH readings was compared by Bland-Altman test.
Condensate CO2 concentration was variable either in neat or argon deaerated samples. There was a close negative logarithmic relation between CO2 and pH (r2>0.99, p<0.01). Calculation of pH at 5.33 kPa CO2 level provided approximately 6 times better reproducibility than the currently used measurements.
Condensate CO2 partial pressure influences pH measurements. Determination of pH at a standard CO2 level provides the most reproducible condensate pH values to date.
Keywords: Airway biology, airway inflammation, breath test, EBC, exhaled biomarkers
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