ERJ
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Published online before print August 22, 2007
Eur Respir J 2007, doi:10.1183/09031936.00067307
This Article
Right arrow Full Text (Rapid PDF)
Right arrow All Versions of this Article:
30/6/1173    most recent
09031936.00067307v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Permissions
Right arrowRequest Permissions
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Losi, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Losi, M.

ORIGINAL ARTICLE

Use of a T-cell interferon-{gamma} release assay for the diagnosis of tuberculous pleurisy

M. Losi 1, A. Bossink 2, L. Codecasa 3, C. Jafari 4, M. Ernst 5, S. Thijsen 6, D. Cirillo 7, M. Ferrarese 3, U. Greinert 4, L.M. Fabbri 1, L. Richeldi 1, C. Lange 4*, for the European tuberculosis network TBNET

1 Section of Respiratory Disease, Dept of Oncology and Hematology, University of Modena and Reggio Emilia, Modena, Italy
2 Dept of Pulmonary Diseases and Tuberculosis, Diakonessenhuis Utrecht, Utrecht, The Netherlands
3 TB Reg. Ref. Centre, Villa Marelli, Niguarda Hospital, Milan, Italy
4 Division of Clinical Infectious Diseases, Research Center Borstel, Germany
5 Division of Immune-cell Analytics, Research Center Borstel, Germany
6 Dept of Medical Microbiology and Immunology, Diakonessenhuis Utrecht, Utrecht, The Netherlands
7 Emerging Bacterial Pathogen Unit, San Raffaele Hospital, Milan, Italy

* To whom correspondence should be addressed. E-mail: clange{at}fz-borstel.de.


  Abstract

The diagnosis of pleural tuberculosis (plTB) by the analysis of pleural effusions (PE) with standard diagnostic tools is difficult.

In routine clinical practice we evaluated the performance of a commercially available M. tuberculosis (MTB)-specific ELISpot assay on peripheral blood mononuclear cells (PBMCs) and pleural effusion mononuclear cells (PEMCs) in patients with suspect plTB.

The T-SPOT.TB (TS.TB) test (Oxford Immunotec Ltd, Abingdon, UK) was performed on PBMCs and PEMCs in 20 patients with a clinical and radiological suspect of plTB and in 21 control subjects with a diagnosis of pleural effusion of non-tuberculous origin at 4 centers participating in the European tuberculosis network (TBNET).

Eighteen of 20 (90%) patients with plTB tested TS.TB-positive on PBMCs and 19/20 (95%) on PEMCs. Among controls, TS.TB was positive in 7/21 patients (33%) when performed on PBMCs (these patients were assumed to be latently infected with MTB) and 5/21 (23%) when performed on PEMCs. Performed on PEMCs, sensitivity and specificity of TS.TB for the diagnosis of active plTB were 95% and 76%, respectively.

Enumerating MTB-specific T-cells in PEMCs by ELISpot is feasible in routine clinical practice and may be useful for a rapid and accurate diagnosis of plTB.

Keywords:  CFP-10, ESAT-6, pleurisy, TIGRA, tuberculosis




This article has been cited by other articles:


Home page
 Eur Respir JHome page
K. Dheda, R. N. van Zyl-Smit, L. A. Sechi, M. Badri, R. Meldau, S. Meldau, G. Symons, P. L. Semple, A. Maredza, R. Dawson, et al.
Utility of quantitative T-cell responses versus unstimulated interferon-{gamma} for the diagnosis of pleural tuberculosis
Eur. Respir. J., November 1, 2009; 34(5): 1118 - 1126.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
C. Lange, M. Pai, F. Drobniewski, and G. B. Migliori
Interferon-{gamma} release assays for the diagnosis of active tuberculosis: sensible or silly?
Eur. Respir. J., June 1, 2009; 33(6): 1250 - 1253.
[Full Text] [PDF]

Home page
 Eur Respir JHome page
U. Mack, G. B. Migliori, M. Sester, H. L. Rieder, S. Ehlers, D. Goletti, A. Bossink, K. Magdorf, C. Holscher, B. Kampmann, et al.
LTBI: latent tuberculosis infection or lasting immune responses to M. tuberculosis? A TBNET consensus statement
Eur. Respir. J., May 1, 2009; 33(5): 956 - 973.
[Abstract] [Full Text] [PDF]

Home page
 LupusHome page
K Takase, S Ohno, H Ideguchi, S Murakami, M Takeno, and Y Ishigatsubo
Mycobacterium tuberculosis-specific immunospot assay of pleural exudate mononuclear cells is useful for the exclusion of tuberculous pleuritis in patients with lupus pleuritis
Lupus, February 1, 2009; 18(2): 175 - 177.
[Abstract] [PDF]

Home page
 CVIHome page
S.-H. Kim, K. Chu, S.-J. Choi, K.-H. Song, H.-B. Kim, N.-J. Kim, S.-H. Park, B.-W. Yoon, M.-d. Oh, and K.-W. Choe
Diagnosis of Central Nervous System Tuberculosis by T-Cell-Based Assays on Peripheral Blood and Cerebrospinal Fluid Mononuclear Cells
Clin. Vaccine Immunol., September 1, 2008; 15(9): 1356 - 1362.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
A. Trajman, M. Pai, K. Dheda, R. van Zyl Smit, A. A. Zwerling, R. Joshi, S. Kalantri, P. Daley, and D. Menzies
Novel tests for diagnosing tuberculous pleural effusion: what works and what does not?
Eur. Respir. J., May 1, 2008; 31(5): 1098 - 1106.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
A. Strassburg, C. Jafari, M. Ernst, W. Lotz, and C. Lange
Rapid diagnosis of pulmonary TB by BAL enzyme-linked immunospot assay in an immunocompromised host
Eur. Respir. J., May 1, 2008; 31(5): 1132 - 1135.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
S. Murakami, M. Takeno, H. Oka, A. Ueda, T. Kurokawa, Y. Kuroiwa, and Y. Ishigatsubo
Diagnosis of Tuberculous Meningitis Due to Detection of ESAT-6-Specific Gamma Interferon Production in Cerebrospinal Fluid Enzyme-Linked Immunospot Assay
Clin. Vaccine Immunol., May 1, 2008; 15(5): 897 - 899.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2007 by the European Respiratory Society.