8-isoprostane as a marker of oxidative stress in non-symptomatic cigarette smokers and COPD

¹ Dept of Medicine and Divisions of Pulmonary Medicine
² Clinical Physiology and
³ Allergology, University of Helsinki and Helsinki University Central Hospital, Helsinki, Finland

^* To whom correspondence should be addressed. E-mail: vuokko.kinnula{at}helsinki.fi.

	Abstract

8-Isoprostane is a potential in vivo marker for oxidant burden, but its usefulness in induced sputum of smokers and COPD has not been investigated.

We investigated 58 subjects, i.e. 11 never smokers, 11 ex-smokers, 13 healthy current smokers, 23 COPD patients with Stage 0-III disease (GOLD criteria). 8-Isoprostane was determined from induced sputum by enzyme immunoassay.

Sputum 8-isoprostane levels were similar in the never-smokers and ex-smokers, but were elevated in the healthy smokers compared to non-smokers (p=0.005) and in Stage I-III COPD (p<0.0001 vs non-smokers and p=0.02 vs healthy smokers). Sputum 8-isoprostane levels could not differentiate non- symptomatic smokers from those with Stage 0 COPD. There was a correlation between the sputum 8-isoprostane level and lung function parameters (FEV₁/FVC r= -0.66, p<0.0001) and sputum neutrophils (r=0.37, p=0.02).

The sputum 8-isoprostane level correlates with the severity of COPD. However, it does not appear to differentiate healthy smokers from those who are at risk for developing COPD (GOLD Stage 0).

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