Figure 8–
Effects of dexamethasone on a) caveolin (Cav)-1, b) endothelial nitric oxide synthase (eNOS) and c) weight gain. a) Immunoblots showing the Cav1 distribution in pooled pulmonary endothelial cell plasma membrane fractions prepared from lungs perfused under control conditions (□), with platelet-activating factor (PAF; 5 nmol) alone (▪), with dexamethasone alone (100 μM) (░), with dexamethasone and PAF (▓) or with quinine (100 μM) and PAF (▒). The pharmacological agents were added after 30 min of perfusion and PAF after 40 min. The perfusion was stopped after 50 min and endothelial cell membrane fractions were prepared. b) The eNOS distribution in the same samples as shown in (a). c) Weight gain in the same experiments as shown in (a). Data are presented as mean±sd (n = 4). *: p<0.05 versus PAF.