Non-cachectic# | Cachectic¶ | |||||
Pre | Post | % change | Pre | Post | % change | |
Muscle fibre % | ||||||
Type I | 32.0±3.2 | 38.7±3.5+ | 6.6±2.3 | 33.6±2.9 | 30.0±6.6 | -3.4±3.6* |
Type IIa | 52.3±3.7 | 51.1±3.9+ | -1.1±2.3 | 50.4±6.5 | 58.0±5.3+ | 9.6±2.8* |
Type IIb | 15.2±2.2 | 9.3±1.4+ | -5.9±1.3 | 15.5±3.6 | 11.9±3.6+ | -5.4±2.1 |
Mean fibre cross sectional area | 4509±198 | 4947±227+ | 10.9±1.7 | 3872±258* | 4136±296+ | 7.3±1.7 |
Cross sectional area μm2 | ||||||
Type I | 4716±271 | 5398 ±315+ | 16.4±4.8 | 4717±215 | 4796±152 | 3.1±4.5* |
Type IIa | 4507±247 | 4954±251 | 11.4±3.5 | 3695±337* | 4091±377+ | 12.1±3.2 |
Type IIb | 3649±208 | 3976±176+ | 11.6±4.3 | 2872±250* | 3224±388+ | 12.1±3.9 |
Capillary/fibre ratio | 1.41±0.1 | 1.70±0.1+ | 20.3±4.1 | 1.44±0.1 | 1.61±0.1+ | 12.1±3.4 |
mRNA IGF-I/GAPDH expression | 0.72±0.11 | 1.29±0.14+ | 79±3 | 0.41±0.09 | 0.77±0.10+ | 88±11 |
mRNA MGF/GAPDH expression | 0.82±0.14 | 1.21±0.13+ | 47±10 | 1.18±0.31 | 2.24±0.71+ | 90±29* |
mRNA MyoD/GAPDH expression | 1.30±2.83 | 2.20±4.16+ | 69±17 | 3.10±1.1 | 2.20±8.7 | -29±6* |
mRNA Myost/GAPDH expression | 2.37±0.48 | 1.78±0.44+ | -25±11 | 2.02±0.48 | 1.96±0.27 | -3±4* |
mRNA TNF-α/GAPDH expression | 0.91±0.27 | 0.62±0.17 | -32±37 | 1.52±0.42 | 1.45±0.55+ | -5±31* |
Data are presented as mean±sem. All mRNA data were normalised against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) number of copies, which were neither significantly different nor affected by exercise training in either of the two groups. IGF-I: insulin-like growth factor-I; MGF: mechano-growth factor; MyoD: myogenic differentiation factor D; Myost: myostatin; TNF-α: tumour necrosis factor-α. #: n = 19; ¶: n = 10. *: p<0.05 compared to non-cachectic; +: p<0.05 within-group differences.