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1 University of Michigan, Ann Arbor, MI, USA. 2 University Paris-Sud, Clamart, France. 3 University of Colorado Health Sciences Center, Denver, CO, USA. 4 Columbia University College of Physicians and Surgeons, New York, NY, USA. 5 Royal College of Physicians, London, UK. 6 University of Bologna, Bologna, Italy. 7 Actelion Pharmaceuticals Ltd, Allschwil, Switzerland. 8 University of California, San Diego, CA, USA
CORRESPONDENCE: V. V. McLaughlin, University of Michigan, 1500 East Medical Center Drive, Women's Hospital, Room L3119, Ann Arbor, MI, USA. Fax: 1 7347637390. E-mail: vmclaugh@med.umich.edu
Keywords: Bosentan, endothelin receptor antagonists, primary pulmonary hypertension, pulmonary hypertension, survival
Received: May 7, 2004
Accepted October 4, 2004
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ABSTRACT |
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 TOP ABSTRACT METHODSRESULTSDISCUSSIONACKNOWLEDGEMENTSREFERENCES |
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Data on survival and alternative treatments were collected from September 1999 (start of the first placebo-controlled study) to December 31, 2002. Observed survival up to 36 months was reported as Kaplan-Meier estimates and compared with predicted survival as determined for each patient by the National Institutes of Health Registry formula.
Kaplan-Meier survival estimates were 96% at 12 months and 89% at 24 months. In contrast, predicted survival was 69% and 57%, respectively. In addition, at the end of 12 and 24 months, 85% and 70% of patients, respectively, remained alive and on bosentan monotherapy. Factors that predicted a worse outcome included World Health Organization Functional Class IV and 6-min walk distance below the median (358 m) at baseline.
First-line bosentan therapy was found to improve survival in patients with advanced primary pulmonary hypertension.
The treatment of primary pulmonary hypertension (PPH), historically viewed as a progressive and fatal disease, has significantly improved over the past decade. Observational studies have demonstrated a mortality benefit in PPH with warfarin anticoagulation 1, 2, while calcium channel blockers improve survival in a small subset of PPH patients who demonstrate marked vasoreactivity 2. In a 12-week randomised, open-label trial of 81 functional class III and IV PPH patients, a survival benefit was demonstrated in patients treated with i.v. epoprostenol compared with patients treated with conventional therapy 3. More recently, two large observational studies have confirmed the long-term mortality benefit among functional class III and IV PPH patients treated with epoprostenol when compared with either historical controls or predicted survival based on the National Institutes of Health (NIH) Registry equation, which incorporates right atrial pressure, cardiac index and mean pulmonary artery pressure 4, 5. While epoprostenol improves exercise tolerance, haemodynamics, and survival, its use is limited by its cumbersome delivery system, which requires continuous i.v. infusion. Deleterious effects, including infection and infusion interruption, may be life threatening.
The dual endothelin receptor antagonist bosentan (Ro 47-0203; Tracleer®, Actelion Pharmaceuticals, Allschwil, Switzerland) is the first oral therapy approved for the treatment of pulmonary arterial hypertension (PAH). Among patients with either PPH or PAH related to the scleroderma spectrum of disease, randomised, double-blind, placebo-controlled studies have demonstrated that bosentan therapy improves haemodynamics, exercise capacity, and functional class 6, 7. In addition, a delay in time to clinical worsening was demonstrated in bosentan-treated patients compared with placebo, with clinical benefits maintained for up to 28 weeks 7. In a long-term, open-label extension study, the improvement in functional class persisted for up to 1 yr 8.
Patients who participated in these studies have now received bosentan as first-line therapy for up to 3 yrs. The objective of this study was to evaluate the effect of a treatment strategy beginning with first-line bosentan therapy, followed by the addition of other therapies if needed, on survival in PPH patients compared with their predicted survival, as determined by the formula of D'alonzo et al. 9 based on NIH Registry data 10. In addition, the use of other targeted treatments, outcomes, and prognostic factors for survival in bosentan-treated PPH patients were explored.
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METHODS |
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TOPABSTRACTMETHODSRESULTSDISCUSSIONACKNOWLEDGEMENTSREFERENCES |
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12 yrs, mean pulmonary arterial pressure >25 mmHg, pulmonary vascular resistance >3 mmHg·L–1·min–1, and pulmonary capillary wedge pressure <15 mmHg as measured by right heart catheterisation, and 6-min walk distance (6MWD) of 150–450 m at baseline. Patients had no previous exposure to prostanoids, and background therapy was based on the discretion of the treating physician. Patients who completed a placebo-controlled study were eligible to continue or start bosentan treatment in the open-label extension study. The target dose of bosentan in the first placebo-controlled trial was 125 mg twice daily. In the second placebo-controlled trial, patients were randomised in a 1:1:1 fashion to placebo, a target dose of 125 mg twice daily, or a target dose of 250 mg twice daily. During the open-label extensions, the target dose was 125 mg twice daily with the option to up-titrate to 250 mg twice daily in cases of clinical deterioration. All studies were conducted in accordance with the amended Declaration of Helsinki at sites in North America, Europe, Australia, and Israel. Studies were approved by a local ethics review committee, and written informed consent was obtained from all patients. Of the 245 patients enrolled in the placebo-controlled studies, PAH was considered primary in 177, and analyses were performed in the 169 PPH patients who received bosentan as first-line therapy for their disease, either during the placebo-controlled study or its extension. Eight patients initially randomised to placebo, seven of whom were withdrawn and treated with alternative therapies and one who died before the end of the placebo-controlled trial, did not receive bosentan as first-line therapy and were excluded from the analysis. Decisions on other treatments were up to the treating physician. During the placebo-controlled studies, patients requiring prostanoid therapy were withdrawn from the study; during the extension studies, prostanoid or other alternate oral therapies could be administered with continued bosentan therapy. Data on vital status and alternative treatments were collected from September 1999 (start of the first placebo-controlled study) to December 31, 2002 (data cut-off), whether or not patients remained on study treatment throughout.
Statistical analyses
Baseline and follow-up information were summarised as mean±SD or frequency counts and proportions. Baseline parameters were recorded at the start of bosentan treatment where possible.
Survival was assessed from the start of bosentan treatment to death or data cut-off. All bosentan-treated PPH patients were included in the analyses (intent to treat); patients lost to follow-up were considered dead at the last known contact. Changes in treatment did not affect the survival analysis. Kaplan-Meier estimates up to 36 months are reported with 99.9% confidence intervals. The annual death rate was obtained by interpolation of the observed data assuming an exponential distribution.
Expected survival was calculated for each patient based on the NIH formula:
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| (001) |
Vital status and treatment at 12 and 24 months were summarised as the proportions of patients who completed 12 and 24 months, respectively. Patients lost to follow-up or with missing information were counted as dead or off bosentan therapy, respectively, at the time of the last available information. Prognostic factors were explored by univariate analysis using the Cox proportional-hazards model; hazard ratios with 95% confidence intervals were reported. The incidence of adverse events and Kaplan-Meier estimates of time to first occurrence of liver transaminase elevation to greater than three times the upper limit of normal were reported for the adverse event observation periods of the four clinical studies.
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RESULTS |
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TOPABSTRACTMETHODSRESULTSDISCUSSIONACKNOWLEDGEMENTSREFERENCES |
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. Although not directly used in the analysis, available data from the NIH Registry of PPH patients are also shown. Patients in the bosentan database were representative of the PPH patient population in the NIH Registry. Although the NIH Registry contained more class II patients than were in the bosentan studies, haemodynamics were similar in both groups. At the time of the last observation, the majority (77.4%) of patients on bosentan were taking bosentan 125 mg twice per day. Altogether, 1% of patients were taking 62.5 mg per day, 11.3% were taking 62.5 mg twice per day, and 10.1% were taking 250 mg twice per day.
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). Mean follow-up was 2.1±0.5 yrs, and 16 patients were followed for >3 yrs. Recorded events included 19 deaths and three lung transplantations; no atrial septostomies were performed. To be conservative, the one patient lost to follow-up was considered dead at the last contact, bringing the number of deaths in the analyses to 20. Similarly, this patient was also considered to have received an alternate therapy at the last contact, as were three patients for whom treatment was unknown during some period. Thus, a total of 39 patients (including the three for whom treatment was unknown and the one who was lost to follow up) were considered to have received a prostanoid derivative (epoprostenol, iloprost, treprostinil) or alternative oral therapy (sitaxsentan, sildenafil) at some time, either instead of or in addition to bosentan. Outcome categories were not mutually exclusive, and patients may have received more than one alternative therapy.
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, table 3). In contrast, the predicted 2-yr survival of these patients based on the NIH equation was 57%. At each 6-month interval, observed survival was significantly better than predicted, as indicated by the 99.9% confidence intervals of the Kaplan-Meier estimates. Overall, patients receiving first-line bosentan therapy had a 5.5% annual death rate. Sixteen patients were followed for more than the 36 months covered in the analysis, and none had died as of the cut-off date.
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). Another 7% at both 12 and 24 months continued bosentan while receiving additional therapy. Of the initial cohort, 78% and 55% were alive and on bosentan monotherapy at 1 and 2 yrs, respectively.
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). Both the magnitude of the hazard ratios and the 95% confidence limits that did not cross 1.0 suggest that baseline functional class and walk distance were associated with a poor outcome. While none of the haemodynamic variables studied were a statistically significant predictor of survival, the trend towards a poor outcome was most relevant for right atrial pressure above the median. Low cardiac index and low pulmonary artery pressure were more weakly correlated with a poor outcome.
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. Adverse events that occurred with a frequency of >10% during this observation period are listed in table 5.
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DISCUSSION |
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TOPABSTRACTMETHODSRESULTSDISCUSSIONACKNOWLEDGEMENTSREFERENCES |
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Epoprostenol was FDA approved for the treatment of PPH in 1995 based on the results of an 81-patient study 3. The primary endpoint of 6MWD improved in patients treated with epoprostenol plus conventional therapy versus those treated with conventional therapy alone, however, a mortality benefit was also observed. Notably, all eight patients who died during the 12-week study period were randomised to conventional therapy alone. These eight patients were also of particularly high risk, with baseline 6MWD of <150 m. It was not until 7 yrs later that the long-term survival benefit of epoprostenol in PPH patients was demonstrated in two large series 4, 5. Although life saving, epoprostenol also has potentially life-threatening adverse effects, with deaths related to infection and infusion interruption.
The mortality benefit of epoprostenol in PPH has prevented other investigational therapies from demonstrating a survival benefit during a 12- to 16-week placebo-controlled trial for two reasons: first, the most critically ill patients, those who would be most likely to die over this short period of time, are generally not enrolled in placebo-controlled trials. They are frequently treated with epoprostenol, as it is considered unethical to enrol such a patient in a placebo-controlled trial. Secondly, if a patient deteriorates during a placebo-controlled trial, they are often withdrawn from the trial and treated with epoprostenol. As a result, longer-term observational studies are required to demonstrate a survival benefit of newer agents.
The present study demonstrates, in a long-term observational manner, that first-line therapy with the dual endothelin receptor antagonist bosentan, followed by addition of other therapy if needed, improves survival in PPH patients. Patients who were enrolled in the two placebo-controlled trials of 12–28 weeks duration were followed for a mean of 2.1±0.5 yrs. The survival at 2 yrs was 89% compared to a predicted survival of 57% based on the equation formulated from the NIH Registry. The NIH Registry predicted survival was used in the present study as it is not possible to follow a contemporary patient population without therapy. Those patients with more advanced symptoms (functional class IV) and lower exercise tolerance (6MWD below the median of 358 m) had a worse outcome with first-line bosentan therapy, similar to what has been demonstrated in the natural history of PPH and in epoprostenol-treated PPH patients 4, 5. The number of functional class IV patients in the current study was small. Given this small sample size, the higher mortality, and the more rapid onset of beneficial effects of epoprostenol (compared with bosentan), it may be appropriate to treat functional class IV patients with epoprostenol, at least initially.
Interestingly, of the prognostic variables studied by univariate analysis, the two noninvasive parameters of functional class and 6MWD were the most powerful predictors of outcome. The prognostic value of functional class has been noted in both untreated PPH patients in the NIH Registry, and in PPH patients treated with epoprostenol 4, 5, 10. Exercise endurance has also been previously correlated with outcome in epoprostenol-treated PPH patients 3–5. While none of the invasive haemodynamic variables reached statistical significance, the strongest trend was noted for right atrial pressure. The unexpected trend towards a poor outcome with a baseline mean pulmonary artery pressure below the median mirrors a previous observation in epoprostenol-treated PPH patients 4. This suggests that mean pulmonary artery pressure may not be the most direct indicator of disease severity, and treatment outcome might be better assessed using other indicators of right ventricular function, such as cardiac index and right atrial pressure, both at baseline and on treatment. Other variables that may have prognostic value in PPH, such as troponin, brain natriuretic peptide, uric acid, and echocardiographic variables were not systematically collected during the placebo-controlled trials and, thus, were not analysed as prognostic variables in the current study.
The favourable safety profile documented in the two placebo-controlled trials appears to persist over the long term. While the incidence of hepatic transaminase elevation was slightly greater than that observed over the 12- to 16-week placebo-controlled trials, there were few new cases after the first 26 weeks on treatment and no serious, irreversible hepatic dysfunction was reported. It is important to note that nearly half (7.2%) of the reported 14.9% of patients with liver function testing abnormalities had elevations in transaminases of greater than five times the upper limit of normal. The importance of following patients monthly for this potentially serious adverse event cannot be overemphasised. Other adverse events noted have commonly been reported in this particularly ill patient population. It should be noted that the length of follow-up for safety was less than that for survival (means of 1.5 and 2.1 yrs, respectively) and thus underestimates the frequency of adverse experiences over the entire follow-up period.
The main limitation of the current study is the use of the NIH Registry equation in lieu of a placebo or historical control group. The NIH equation is based on data from the 1980s, and background practice patterns, particularly the widespread use of oral anticoagulation, may have changed since then. However, studies demonstrating a survival benefit with oral anticoagulation have not been performed in a prospective and controlled matter. In fact, a recent evidence-based review only gave a moderate recommendation for the use of oral anticoagulation in patients with PPH 13. Although the method used in the validation of the NIH equation was crude by later standards 14, 15, more recently published estimates of survival in PPH remain similar to the expected survival based on the NIH equation. In an evidence-based literature review conducted via a search of the Medline bibliographic database from 1992 to October 2002, 12–21 studies were identified that described 1, 2, or 3-yr survival of patients with PPH 16. Unweighted and unadjusted averages of the entire data set demonstrated a survival of 79% at 1 yr, 66% at 2 yrs, and 59% at 3 yrs. The same evidence-based review also described survival curves for PPH patients treated with conventional medical therapy versus epoprostenol during that time period. The 1, 2, and 3-yr survival was 72%, 53%, and 48%, respectively, in those patients who did not receive epoprostenol versus 82%, 74%, and 62%, respectively, in those patients who did receive epoprostenol. The reported survival in patients treated with conventional therapy is similar to the estimated survival based on the NIH equation that is used in the current study. Another limitation of this study is that patients were initially selected for participation in a placebo-controlled trial. Certainly, patients who participate in trials may not reflect the patients that clinicians most commonly see on an everyday basis.
Given the progressive nature of PPH, it is unethical to perform a placebo-controlled trial of 2–3 yrs duration. Additionally, during the extension studies other assessments of disease severity, such as 6MWD, haemodynamics, and functional classification were not systematically collected. It would have also been interesting to have followed other prognostic variables, such as brain natriuretic peptide or troponin. Documentation of such results could have substantially strengthened the results of the current study. Practice variations are another limitation. During the open-label extensions following the placebo-controlled trials, treatment patterns were not mandated by protocol and were left to the discretion of the investigator. It is important to note that these results are not those of bosentan therapy alone, but rather of a treatment strategy starting with bosentan. At the discretion of the investigator, a prostanoid or other therapy for PPH could have been added to or substituted for bosentan, after the placebo-controlled portion of the study. However, this reflects current variability in practice patterns and is a practical approach to the therapy of PPH.
In conclusion, the present study suggests that the treatment of first-line bosentan therapy, followed by the addition of other pulmonary arterial hypertension therapies if needed, improves survival in patients with advanced primary pulmonary hypertension. Given the ease of administration and favourable side-effect profile, the strategy of treatment with first-line bosentan should be considered for World Health Organization functional class III primary pulmonary hypertension patients.
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ACKNOWLEDGEMENTS |
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TOPABSTRACTMETHODSRESULTSDISCUSSIONACKNOWLEDGEMENTSREFERENCES |
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FOOTNOTES |
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REFERENCES |
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TOPABSTRACTMETHODSRESULTSDISCUSSIONACKNOWLEDGEMENTSREFERENCES |
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: dead; 
: alive, not on bosentan; 
: alive, on bosentan and other treatments; 
: alive, on bosentan alone.