| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Depts of Respiratory 1 Medicine, Respiratory Centre, and 2 Pathology, Toranomon Hospital, Tokyo, Japan
CORRESPONDENCE: S. Homma, Dept of Respiratory Medicine, Toranomon Hospital, Toranomon 2-2-2, Minato-ku, Tokyo, 105-8470, Japan. Fax: 81 335827068. E-mail: sa-homma@toranomon.gr.jp
Keywords: Cholesterol granuloma, human, indium-tin oxide, lung injury, pulmonary fibrosis
Received: February 2, 2004
Accepted June 17, 2004
ABSTRACT
Despite the increasing industrial use of indium-tin oxide (ITO) to manufacture flat-panel displays, such as liquid-crystal displays or plasma display panels for televisions, little is known about the potential health hazard induced by occupational exposure to indium compounds.
The current study describes a case of fibrotic lung disease that developed after a 4-yr exposure to ITO. The pathology of the lung demonstrated pulmonary fibrosis with the presence of cholesterol granulomas.
In conclusion, more attention needs to be paid to the possible toxic effects of indium compounds, and maximum healthcare measures should be taken to protect industry workers from these toxicities.
CASE PRESENTATION
In January 2002, a 30-yr-old male engineer visited the current authors' hospital (Dept of Respiratory Medicine, Respiratory Centre, Toranomon Hospital, Tokyo, Japan) complaining of dry cough and exertional dyspnoea, which he had been experiencing since 1997. He had a minimum history of cigarette smoking (3 cigarettes·day–1 for 3 yrs). From 1994 to 1998, he had been exposed to indium compounds (i.e. ITO) as an aerosol while making transparent conductive films. Physical examination of the chest on admission revealed normal vesicular breath sounds and the absence of finger clubbing. Pulmonary function tests and arterial blood gas analysis in room air yielded normal values: a vital capacity of 4.18 L (93% predicted), forced expiratory volume in one second of 3.07 L (73% pred), residual volume (RV) of 2.20 L (measured by the gas dilution method; 158% pred), total lung capacity (TLC) of 6.38 L (109% pred), RV/TLC ratio of 34%, carbon dioxide diffusing capacity of the lung of 3.21 mL·min–1·kPa (89% pred), pH 7.40, arterial oxygen tension of 11.8 kPa, and arterial carbon dioxide tension of 5.3 kPa 10. Peripheral blood examination showed that the counts of leukocytes and red blood cells, as well as the level of C-reactive protein, were normal, while KL-6, as an indicator of active pneumonitis, rose slightly to 799 U·mL–1 (normal values <500 U·mL–1) 11. The serum indium level measured by inductively coupled plasma mass spectrometry (Agilent Technologies, Palo Alto CA, USA) was high at 51 µg·L–1 (normal values <0.1 µg·l–1) 12. Chest roentgenogram on admission showed reticulonodular shadows in the right upper lung field. On chest computed tomography (CT), diffuse ground-glass opacities were present adjacent to the peripheral air space consolidation in the right upper lobe. Diffusely scattered centrilobular fine nodular densities in both lung fields associated with emphysematous low attenuation areas at both apexes were also seen (fig. 1
). An oedematous, reddish mucosa in the entire bronchial tree was observed by means of flexible bronchoscopy. Bronchoalveolar lavage performed through the right B5 bronchus revealed a minor increase in lymphocytes: 300,000 cells·mL–1 (macrophages 77.8%, lymphocytes 18.8% (CD4+/CD8+ ratio of 1.14), neutrophils 2.4%, eosinophils 0.6%, basophils 0.4%).
|
). There were no lobuli without injury in either specimens from the right S2 and S8 segments. In addition to these findings, diffuse alveolitis with intra-alveolar accumulation of alveolar macrophages containing brown particles was observed. Electron probe X-ray microanalysis 13, field emission-scanning electron microscopy (Hitachi Co. Ltd., Tokyo, Japan), and energy dispersive X-ray analytical spectrometer (Kevex Sigma, Trento, Italy) studies on these lung tissues confirmed that the particles contained a large amount of indium (61%) and a small amount of tin (4%; fig. 3).
|
|
DISCUSSION
There have been a relatively limited number of reports in the literature describing pulmonary toxicities of inhaled indium-containing materials, such as indium arsenide or indium phosphide, in animals as well as humans 3–9. However, only one report regarding experimental pulmonary toxicity of ITO has been published to date 4. In the study by Tanaka et al. 4, the pulmonary toxic effect of ITO and indium phosphide was evaluated by repeated instillation into the trachea of hamsters. In the ITO group, the inflammatory response was milder than that observed in the indium phosphide group; for example, inflammatory foci seen in the lung were less prominent. However, in the same ITO group, numerous alveolar macrophages with expanded cytoplasm containing various amounts of brown ITO particles, necrotic cell debris or a small number of neutrophils were present within the alveolar septa, alveolar spaces or bronchiolar lumens. The formation of cholesterol granulomas was not observed, although cholesterol clefts were slightly present in that animal model. Since ITO contains a high fraction of indium (indium 74%; tin 8%), the main cause of the observed lung toxicity was probably indium.
Prior to the present report, only one human case of interstitial pneumonia caused by inhaled ITO particles has been documented by Homma et al. 3. The patient described came from the same factory as in the current study and died of bilateral pneumothorax after a 4-yr illness. He had been engaged in production of transparent conductive films made of ITO for flat-panel displays. The autopsy demonstrated interstitial pneumonia with numerous and defined fine particles scattered throughout both lungs. In addition, his alveolar spaces were filled with red blood cells, fibrin, cholesterol clefts and alveolar macrophages with expanded cytoplasm containing fine particles. Intrapulmonary deposition of indium and tin was shown by X-ray energy spectrometry in the fine particles, as it was in the present case. The level of serum indium was extremely high (290 µg·L–1). Thus, the reported case was diagnosed as having interstitial pneumonia caused by the inhalation of ITO particles.
Likewise, the patient in the present report had been engaged in the same metal plant as the first case for 4 yrs, had a high serum indium level (51 µg·L–1), and the pathological findings observed in the biopsied lung specimens, such as subpleural emphysema and the presence of numerous cholesterol granulomas, were reminiscent of those in the previously reported case. The patient in the current study has not yet developed pneumothorax, suggesting that emphysematous changes may be the late phenomenon of the progression of peribronchial fibrosis in the centrilobular regions.
ITO is a sintered alloy containing a large portion of indium oxide and a small portion of tin oxide. The mean count diameter of the ITO particles, as sampled in the workplace, was 1.0 µm. Both of these two cases were engaged in wet surface grinding of ITO target under the protection of wearing a dust mask. According to Chonan and Taguchi 14, among 115 workers who were involved in this job in the same metal plant, 14 individuals revealed interstitial fibrosis on chest CT. Although the solubility of ITO particles both in vitro and in vivo has not been well documented, the high concentration of indium in the patients' sera indicated indium dissolution from ITO particles. Indium in the blood seems to be distributed to multiple organs and may cause chronic adverse health effects. For example, the first human case reported by Homma et al. 3 showed liver damage and splenomegaly. Testicular toxicity caused by intratracheal instillation of ITO was also demonstrated recently in hamsters 15. However, the hazardous effects of the ITO particles appeared to be milder than those of indium phosphide in hamsters 4.
In these two cases, the common characteristic pathological feature is the presence of numerous cholesterol granulomas in both the alveolar spaces and the interstitium. In general, the formation of pulmonary interstitial and intra-alveolar cholesterol granulomas is the consequence of fibrous organisation of cholesterol crystals originating from lipid-laden macrophages or endogenous lipids in lipoid pneumonia 16. Lipoid pneumonia itself can be divided into endogenous and exogenous diseases 17. Kay et al. 18 also proposed that pulmonary cholesterol-ester granulomas in man might be the result of hyperplasia and desquamation of granular pneumocytes. However, in the present case, no apparent cause of either the exogenous or endogenous disease could be detected. In addition, histopathological examinations also showed no evidence of hyperplasia or desquamation of granular pneumocytes. Based on the above discussion, the current authors propose that the characteristic primary lesion caused by inhalation of ITO comprises the formation of interstitial and intra-alveolar cholesterol granulomas in the fibrotic lung, although the precise mechanism of the pathological processes responsible for the disease remains to be elucidated.
Finally, more comprehensive assessment of a population of indium-tin oxide workers is needed, and maximum healthcare measures should be taken to protect them from the potential toxicities of occupational exposure to indium compounds.
|
The authors would like to thank E. Suzuki of Niigata University, T. Gotoh and K. Matsumoto of Waseda University and H. Taniguchi of the University of Tokyo for technical support in electron probe X-ray microanalysis, field emission-scanning electron microscopy and energy dispersive X-ray analytical spectrometer studies. The authors also thank the Japan Industrial Safety and Health Association for measuring the serum indium level.
REFERENCES
This article has been cited by other articles:
|
|
 |
|
  T Hamaguchi, K Omae, T Takebayashi, Y Kikuchi, N Yoshioka, Y Nishiwaki, A Tanaka, M Hirata, O Taguchi, and T Chonan Exposure to hardly soluble indium compounds in ITO production and recycling plants is a new risk for interstitial lung damage Occup. Environ. Med., January 1, 2008; 65(1): 51 - 55. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Chonan, O. Taguchi, and K. Omae Interstitial pulmonary disorders in indium-processing workers Eur. Respir. J., February 1, 2007; 29(2): 317 - 324. [Abstract] [Full Text] [PDF]
|
|
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
