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Eur Respir J 2004; 24:333
Copyright ©ERS Journals Ltd 2004

Additive blockade of ß2-integrin adhesion of eosinophils by salmeterol and fluticasone propionate

S.P. Hart

MRC Centre for Inflammation Research, University of Edinburgh Medical School, Edinburgh, UK

To the Editor:

In their report of the effects of fluticasone propionate and salmeterol on eosinophil adhesion, Myo et al. 1 correctly considered that fluticasone propionate may have reduced eosinophil adhesion by inducing apoptosis 2–5, but they didnot perform the appropriate experiments to test this. Assessment of "cell viability" by exclusion of membrane-impermeant dyes such as trypan blue or propidium iodide will not identify apoptotic eosinophils, which generally have intact plasma membranes 6. Appropriate methods to quantify eosinophil apoptosis include examination of nuclear morphology and annexin V staining, which if performed would probably have resulted in a different interpretation of the data. Apoptotic cells are clearly not "viable" because cellular functions (including cell adhesion) are significantly impaired. Unfortunately, the term "cell viability" is widely used in scientific literature, but it is imprecise and should be abandoned.

References

  1. Myo S, Zhu X, Myou S, et al. Additive blockade of ß2-integrin adhesion of eosinophils by salmeterol and fluticasone propionate. Eur Respir J 2004;23:511–517.[Abstract/Free Full Text]
  2. Whyte MKB, Meagher LC, MacDermot J, Haslett C. Impairment of function in aging neutrophils is associated with apoptosis. J Immunol 1993;150:5124–5134.[Abstract]
  3. Dransfield I, Stocks SC, Haslett C. Regulation of cell adhesion molecule expression and function associated with neutrophil apoptosis. Blood 1995;85:3264–3273.[Abstract/Free Full Text]
  4. Meagher LC, Cousin JM, Seckl JR, Haslett C. Opposing effects of glucocorticoids on the rate of apoptosis in neutrophilic and eosinophilic granulocytes. J Immunol 1996;156:4422–4428.[Abstract]
  5. Zhang X, Moilanen E, Kankaanranta H. Enhancement of human eosinophil apoptosis by fluticasone propionate, budesonide, and beclomethasone. Eur J Pharmacol 2000;406:325–332.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  6. Stern M, Meagher LC, Savill JS, Haslett C. Apoptosis in human eosinophils: programmed cell death in the eosinophil leads to phagocytosis by macrophages and is modulated by IL-5. J Immunol 1992;148:3543–3549.[Abstract]




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