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Exhaled breath condensate: a space odessey, where no one has gone before...

¹ Dept of Pediatrics, Maasland Hospital, Sittard, and ² Dept of Pediatric Pulmonology, University Hospital Maastricht, Maastricht, the Netherlands

To the Editor:

We read with great interest the editorial of Rahman 1 on the reproducibility of oxidative stress biomarkers in breath condensate. As a consequence, we would like to share some of our thoughts, as ultimately, we all may want to walk on planet Mars. However, this does not mean that we are already able to lift off, as many methodological problems first need to be properly addressed. Similarly, exhaled breath condensate (EBC) is an interesting noninvasive technique to explore inflammatory lung diseases, where no one has gone before... Many methodological issues are still waiting to be solved, as recently reviewed 2. Indeed, the development of EBC is currently hampered by many conflicting reports on biomarker reproducibility. As clearly stated, one of the main obstacles consists of current analytical problems, due to limitations of sensitivity and specificity of the assays used to date 3.

However, the statement that "now with the use of EcoScreen, collection of EBC is being standardised in many leading laboratories" may be misleading, as it suggests that "this would no longer be a confounding factor contributing to the variations in biomarkers in EBC" 1. We want to clearly point out that the EBC collection method still remains a possible confounding factor and an important source of biomarker variability, because standardisation involves applying more than one identical collection technique. To our knowledge, there is no scientific evidence that the EcoScreen condenser would be the most valid technique to collect EBC for the measurement of inflammatory mediators in condensate. In fact, the key issue is not the reproducibility of a certain biomarker, but the reproducibility of a certain biomarker for a certain condenser system 2. We compared the influence of different inner condenser coatings on the detection of human albumin and 8-isoprostane in EBC 4. Our data show a much greater efficiency of condenser systems with a borosilicate glass or silicone coating, compared with the EcoScreen or condensers with aluminium, polypropylene and teflon coating. This implicates that the EcoScreen may not be the most valid apparatus, at least not for some biomarkers.

Although the need for clear-cut methodological recommendations is incontestable, one has to recognise that we are not yet able to give such recommendations. Further research on the reproducibility of biomarkers with different condenser systems is urgently needed.

References

1. Rahman I. Reproducibility of oxidative stress biomarkers in breath condensate: are they reliable? Eur Respir J 2004;23:183–184.[Free Full Text]
2. Rosias PPR, Dompeling E, Hendriks HJE, Heijnens JWCM, Donckerwolcke RAMG, Jöbsis Q. Exhaled breath condensate in children: pearls and pitfalls. Pediatr Allergy Immunol 2004;15:4–19.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
3. Van Hoydonck PGA, Wuyts WA, Vanaudenaerde BM, Schouten EG, Dupont LJ, Temme EHM. Quantitative analysis of 8-isoprostane and hydrogen peroxide in exhaled breath condensate. Eur Respir J 2004;23:189–192.
4. Rosias PPR, Vernooy JHJ, Dentener MA, et al. The inner coating of condenser systems influences the detection of human albumin in exhaled breath condensate. Eur Respir J 2003;22: Suppl. 45, 280s.

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