HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (26) Citing Articles via Google Scholar Google Scholar Articles by Mendes, E.S. Articles by Wanner, A. Search for Related Content PubMed PubMed Citation Articles by Mendes, E.S. Articles by Wanner, A.

Comparative bronchial vasoconstrictive efficacy of inhaled glucocorticosteroids

¹ Pulmonary and Critical Care Division, Dept of Medicine, University of Miami at Mount Sinai and ² Dept of Epidemiology and Public Health, University of Miami, Miami, FL, USA

CORRESPONDENCE: A. Wanner, Division of Pulmonary and Critical Care Medicine, University of Miami School of Medicine, P.O. Box 016960 (R-47), Miami, FL 33101, USA. Fax: 1 3052436992. E-mail: awanner@miami.edu

Keywords: asthma, bronchial blood flow, corticosteroid, vasoconstriction

Received: August 8, 2002
Accepted February 10, 2003

This research was supported by an academic research grant from Glaxo Inc., Middlesex, UK.

	Abstract

TOP Abstract Methods Results Discussion References

 
The vasoconstrictive efficacies of glucocorticosteroids (GS) are usually compared by the McKenzie skin-blanching test and taken as an index of relative potency. The rationale for the present study was to transpose the McKenzie test to the airway and to compare the airway vascular effects of three inhaled GS: beclomethasone dipropionate (BDP), fluticasone propionate (FP) and budesonide (BUD), in healthy subjects and patients with mild stable asthma.

A soluble, inert gas-uptake method was used to measure airway blood flow (Q_aw). Baseline mean±sd Q_aw normalised for anatomical dead space was 53.1±1.4 µL·min^–1·mL^–1 in healthy subjects (n=10) and 67.8±3 µL·min^–1·mL^–1 in asthmatics (n=10).

All GS caused a transient decrease in Q_aw. The magnitude of the vasoconstriction was greater in asthmatics. The relative vasoconstrictive effect of BDP, FP and BUD was 1, 1.9, and 2.7, respectively, in asthmatics and 1, 3.3 and 3.0, respectively, in healthy subjects, as assessed by the dose required to decrease Q_aw by 20% from the baseline, 30-min postdrug inhalation.

Therefore, measuring airway blood flow may be a useful, site-specific parameter to assess the tissue bioavailability and vasoconstrictive efficacy of inhaled glucocorticosteroids.

Inhaled glucocorticosteroids (GS) have assumed an important role in the treatment of asthma. Various outcome measures have been used to quantitate the clinical efficacy (maximum effect) of inhaled GS 1–4. However, efficacy cannot be equated with potency (receptor-binding affinity). However, both efficacy and potency are important in establishing a therapeutic index. The in vivo "potency" (tissue bioavailability and vasoconstrictive efficacy) of inhaled GS is commonly determined by the McKenzie skin-blanching test 5. The test is based on the ability of inhaled GS to cause transient cutaneous vasoconstriction when applied topically in healthy subjects. However, for inhaled GS, the McKenzie skin-blanching test is not ideal because the test is performed in the skin as a substitute for the airway, it is time-consuming and involves normal rather than inflamed tissue. The latter is considered a shortcoming as airway inflammation has been shown to alter the responsiveness to vasoactive agents 6. Airway tissue is the therapeutic target for inhaled GS. Therefore, the airway can be considered the ideal site to assess the tissue bioavailability and vasoconstrictive efficacy of inhaled GS.

The authors have developed and validated an in vivo technique for the measurement of airway blood flow (Q_aw) inhumans 7. They have shown that Q_aw is increased inasthmatics, presumably due to inflammatory new vessel formation and vasodilatation 6, 7. They also found that inhaled fluticasone propionate (FP) causes a dose-dependent, transient decrease in Q_aw in healthy and asthmatic subjects, with a greater response observed in the latter 8, 9. These findings indicate that Q_aw is a suitable index of tissue bioavailability and vasoconstrictive efficacy for inhaled GS.

The purpose of the present study was to compare the vasoconstrictive effect of three commercially available inhaled GS preparations: FP, budesonide (BUD) and beclomethasone dipropionate (BDP), in healthy and asthmatic subjects.

	Methods

TOP Abstract Methods Results Discussion References

 
Test subjects
A total of 10 subjects with mild asthma and 10 normal volunteers, without any history of asthma or other respiratory disease, were recruited for this study. All subjects were current nonsmokers. Asthma was defined by American Thoracic Society criteria 10. At study entry, all asthmatics were clinically stable and had a forced expiratory volume in one second (FEV₁) >70% predicted, rare daytime symptoms and no nocturnal awakenings (mild intermittent asthma 11). The asthmatics had not used inhaled or systemic GS or regularly administered ß-adrenergic agonists for a minimum of 2 weeks before the study.

None of the subjects were taking oral anti-inflammatory agents or vasoactive medications, or had cardiovascular disease. All subjects denied having experienced an acute respiratory infection <1 month before the study and no subject developed an acute respiratory infection during the study.

The study protocol was approved by the Mount Sinai Medical Center and the University of Miami Institutional Review Boards. All subjects provided written, informed consent. They received financial remuneration for their participation.

Forced expiratory volume in one second
Spirometry was carried out using an Essential Medic Unit (model 6200 Autobox DL; Yorba Linda, CA, USA). The FEV₁ was determined and expressed as an absolute value and as % pred 12.

Airway blood flow
A previously validated and applied, soluble, inert gas-uptake was used to measure Q_aw 7, 13. The subjects first inhaled room air and then exhaled 500 mL from the total lung capacity position. Subsequently, the subjects rapidly inhaled the same volume of gas from a gas mixture contained in aTeflon bag, consisting of 10% dimethylether (DME), 5% helium and a balance of oxygen. After a predetermined breath-hold time, the subject then exhaled into a spirometer, through a critical flow orifice, to standardise the expiratory flow. During exhalation, the instantaneous concentrations of DME, nitrogen and helium were measured at the airway opening using a mass spectrometer (Perkin-Elmer, Pomona, CA, USA) along with the expired gas volume. The manoeuvre was performed with two breath-hold times each of 5, 10, 15and 20 s in random order. The Q_aw was calculated by multiplying the helium-corrected DME concentration slope by the expired anatomic dead space volume (minus the proximal 50 mL) to obtain DME uptake, which was then divided by the mean DME concentration and the solubility coefficient for DME in blood (Fick's principle). The anatomic dead space was derived from the nitrogen washout curve inscribed after the 10-s breath-hold time. Q_aw was expressed as µL·min^–1·mL^–1 anatomical dead space.

Protocol
The subjects were instructed to abstain from ingesting alcoholic beverages the night before the study and the asthmatic subjects were asked not to use their inhaled ß-adrenergic agonist for 12 h before the study. For each subject, the experiment was started at the same time on the different study days. The subjects were asked not to ingest coffee or caffeinated drinks prior to the study.

Days 1–3: time course
The subjects inhaled either BDP (1,680 µg), FP (880 µg) or BUD (1,000 µg) from commercially available metered-dose inhalers (MDIs) with a spacer in random order on three different days. The manoeuvre was standardised by having the subjects inhale from a functional, residual-capacity position to a total lung-capacity position, followed by a 10-s breath-hold. The Q_aw and FEV₁, blood pressure and pulse rate were measured before and at 15-, 30-, 60-, 90- and 120-min postdrug inhalation.

Days 4–6: dose/response
On each of these 3 days, the subjects underwent a dose/response assessment of one of the three GS preparations in random order. The doses were as follows: 420, 840, 1,680 and 3,360 µg for BDP; 220, 440, 880 and 1,760 µg for FP; and 200, 400, 800 and 1,600 µg for BUD. After the baseline measurements of blood pressure, pulse rate, Q_aw and FEV₁, the lowest dose was inhaled and the measurements repeated 30 min later. This corresponded to the nadir of Q_aw after inhalation of FP in a previous study 9. Immediately thereafter, the next highest dose was inhaled and the procedure repeated until the maximum dose had been inhaled.

Statistical analysis
The unpaired and paired variants of t-tests were used to compare baseline data among and within the two groups. A univariate and multivariate, repeated-measures analysis were used to analyse and compare the time courses to the three drugs. To assess the dose/response, the appropriate statistical model was used, analysing the response within each subject and pooling across subjects, using the method of dummy variables to adjust for different levels of response across subjects.

The response profiles were modelled within subjects as:

(001)

where i is the subject number, a the intercept, b the decay constant and e the base of natural log, using a natural log transform to ln(Q_aw i)=ln(a_i)–bDose. Use of the dummy variables allowed the computation of a common decay constant b. The final model was:

(002)

where a is back-calculated from the average of the baseline ln(Q_aw) across subjects. The adequacy of this procedure was assessed by the R² value associated with the final model.

Once the model was computed for each drug and each group, the per cent reduction from baseline was calculated to find an appropriate point within the range of the data to estimate relative vasoconstrictive efficacy. For a given per cent of baseline (P), the dose necessary to reach that point was calculated as:

(003)

Relative vasoconstrictive efficacy was calculated as the ratio of the respective Dose_p values. The sd was derived according to the principle of the propagation of error. Data were expressed as mean±sd. A p<0.05 was considered significant.

	Results

TOP Abstract Methods Results Discussion References

 
Demographic and baseline physiological data for the two study groups are shown in table 1. The mean Q_aw was 29% higher in subjects with asthma when compared with healthy subjects (p<0.001). The mean FEV₁ was lower in subjects with asthma compared with the healthy subjects (p<0.001). All other parameters were similar in the two groups of subjects. There were no statistically significant variations in baseline Q_aw among experimental days (fig. 1). Likewise, mean baseline blood pressure, pulse rate, and FEV₁ remained stable throughout the study.

View larger version (30K): [in this window] [in a new window]   Fig. 1.— Baseline airway blood flow (Qaw) in healthy () and asthmatic subjects (). Data are presented as mean±sd.

No adverse effects were reported after drug inhalation. Some subjects experienced transient sleepiness that was attributed to DME.

Time course of glucocorticosteroid effect on airway blood flow
All subjects showed a marked, statistically significant decrease in Q_aw after inhalation of BDP, FP and BUD (figs 2 and 3). The nadir in mean Q_aw was observed at 60 min in all instances except for BUD in healthy subjects, where the nadir occurred at 90 min. As the time of maximal decrease in Q_aw showed interindividual variability, the authors calculated the maximum change in Q_aw for each subject. The mean maximum decrease was greater in asthmatics compared with healthy subjects for all three GS examined (p<0.05; table 2).

View larger version (12K): [in this window] [in a new window]   Fig. 2.— Airway blood flow (Qaw) before and after the inhalation of fluticasone (•), beclomethasone () and budesonide () in healthy subjects (n=10). Data are presented as mean±sd. *: p<0.05 versus baseline value.

View larger version (13K): [in this window] [in a new window]   Fig. 3.— Airway blood flow (Qaw) before and after the inhalation of fluticasone (•), beclomethasone () and budesonide () in asthmatic subjects (n=10). Data are presented as mean±sd. *: p<0.05 versus baseline value.

View larger version (13K): [in this window] [in a new window]   Fig. 4.— Dose/response relationship between inhaled fluticasone (•), beclomethasone () and budesonide () doses and airway blood flow (Qaw) in healthy subjects (n=10). Data are presented as mean±sd. *: p<0.05 versus baseline value.

View larger version (13K): [in this window] [in a new window]   Fig. 5.— Dose/response relationship between inhaled fluticasone (•), beclomethasone () and budesonide () dose and airway blood flow (Qaw) in asthmatics (n=10). Data are presented as mean±sd. *: p<0.05 versus baseline value.

	Discussion

TOP Abstract Methods Results Discussion References

There is no method to evaluate the potency of inhaled GS in vivo. The standard screening test to determine the relative "potencies" for inhaled GS has been the McKenzie skin-blanching test 5. However, this procedure has come under criticism. The intensity of the blanching response to a GS varies from subject-to-subject and is influenced by ambient temperature and humidity and other factors 16. The blanching effect score is difficult to compare among groups or among specific formulations conducted in different groups and the visual reading of the skin-blanching by the human eyeis a subjective measure. Furthermore, the results are influenced by drug diffusibility through tissue, which is likely to vary in skin and in the airway. Most importantly, the test assesses vasoconstrictive efficacy rather than true potency (receptor-binding affinity) and may have little relevance to theanti-inflammatory effects of GS, which, in contrast tovasoconstriction, are mediated through genomic effects. Finally, aerosol delivery devices can produce clinically significant differences in topical activity by altering the dose deposited in the lung 17. Alternative approaches are therefore desirable.

The authors believe that the quantitative assessment of bronchial vasoconstriction, as described in this article, is such an alternative approach. The vascular response is measured atthe anatomically relevant site for inhaled GS, using a clinically relevant inhalation technique and with the inclusion of clinically relevant test subjects, such as asthmatics.

Inhalation technique, particle size distribution and airway geometry all influence aerosol deposition in the airways. Although the inhalation manoeuvre was normalised and a spacer was used, MDI-generated, particle-size distribution may have differed for the three drugs used. Asthmatic and healthy subjects have different airway geometry, which, in turn, influences total aerosol deposition and deposition distribution. These variables are part of tissue bioavailability and may significantly influence the authors' vasoconstrictive efficacy measurement for the three MDIs used clinically.

The change of Q_aw in response to inhaled GS is a physiological parameter that reflects bronchial vascular tone because inhaled GS have no effect on bronchial arterial perfusion (aortic) and presumably downstream (atrial) pressures. Therefore, there is a direct relationship between vascular conductance (which is indirectly related to bronchial vascular smooth muscle tone) and Q_aw. It is unlikely that Q_aw was influenced by the manoeuvres involved in this study's protocol, based onprevious observations with a chlorofluorocarbon placebo MDI 9.

The dose/response measurements were taken at 30-min intervals because the authors had previously demonstrated that FP at 880 µg produced a transient decrease in mean Q_aw with a nadir at 30 min in asthmatic and healthy subjects 9 and because it was the first time point where all three drugs showed a significant change in Q_aw in healthy and asthmatic subjects. Analysis of the dose/response curve at 60 min may have changed the relative vasoconstrictive efficacy ratio for the three drugs.

The relative vasoconstrictive efficacies of BDP, FP and BUD were 1, 1.9 and 2.7, respectively, in asthmatic subjects and 1, 3.3 and 3.0, respectively, in healthy subjects. Using the McKenzie skin-blanching test, Kamada et al 18 found the vasoconstrictive efficacy of inhaled GS relative to dexamethasone to be 600 for BDP, 980 for BUD and 1,200 for FP 18. While different relative efficacies have been reported by other investigators, the rank order of BDP<BUD<FP has been consistent across laboratories 19, 20. The discrepancies may result from differences in the technique of applying the drug to the skin or the subjective assessment of skin blanching. The interdrug quantitative differences between the present observations and the reported skin-blanching findings may be related to the differences in tissue bioavailability.

The mechanism of the rapid transient vasoconstrictor response to topically applied GS is unknown. Preliminary findings suggest that -adrenergic neurotransmission is involved 21. The concentration of noradrenaline (NA) at ₁-adrenergic receptor sites is, in part, regulated by NA uptake into postsynaptic cells (extraneural uptake: uptake₂) where NA undergoes enzymatic inactivation. It has been demonstrated in vitro that, uptake₂ is inhibited by steroid hormones through a nongenomic action 22. This could lead to an increased NA concentration at the neuromuscular junction and explain the GS-induced vasoconstriction. The potentiated GS-induced vasoconstriction in asthmatics' airways seems to be accompanied by a greater ₁-adrenergic vasoconstrictor response 6. This adds further support to an -adrenergic-GS interplay in the regulation of vascular tone.

The Q_aw response to inhaled GS is an in vivo index of airway tissue bioavailability and vasoconstrictive efficacy, not true potency or clinical anti-inflammatory efficacy. Although the vasoconstrictive differences reported here, for three popular inhaled GS, may not affect their clinical efficacy, the authors believe it is essential to consider tissue bioavailability when clinically evaluating GS. The Global Initiative for Ashma guidelines, recommend dosages for different inhaled GS on a µg·day^–1 basis, implying that no differences exist among the products 23. However, both in vitro and in vivo evaluations, including the present study, have demonstrated interdrug differences in potency and bioavailability 18–20. Such data are needed to establish a therapeutic index 20, 24.

In summary, this investigation showed that inhaled fluticasone propionate and budesonide cause greater vasoconstriction inthe airway than beclomethasone dipropionate. It was also shown that for all three inhaled glucocorticosteroids, the vasoconstrictor response is greater in asthmatics than in healthy subjects. These findings indicate drug-specific and disease-specific in vivo potency differences in both bioavailability and vasoconstrictive efficacy among three commonly prescribed glucocorticosteroid metered-dose inhalers.

	References

TOP Abstract Methods Results Discussion References

 

1. Martin RJ, Szefler SJ, Chinchilli, et al. Systemic effect comparisons of six inhaled corticosteroid preparations. AmJ Respir Crit Care Med 2002;165:1377–1383.[Abstract/Free Full Text]
2. Nielsen LP, Dahl R. Therapeutic ratio of inhaled corticosteroids in adult asthma. Am J Respir Crit Care Med 2000;162:2053–2057.[Abstract/Free Full Text]
3. Barnes PJ. Inhaled glucocorticosteroids for asthma. N Engl J Med 1995;332:868–875.[Free Full Text]
4. Djukanovic R, Wilson JW, Britten K, et al. Effect of an inhaled corticosteroid on airway inflammation and symptoms in asthma. Am Rev Respir Dis 1992;145:669–674.[ISI][Medline] [Order article via Infotrieve]
5. McKenzie AW, Stoughton RB. Method for comparing percutaneous absorption of steroids. Arch Dermatol 1962;86:608–610.[ISI]
6. Brieva J, Wanner A. Adrenergic airway vascular smooth muscle responsiveness in healthy and asthmatic subjects. J Appl Physiol 2001;90:665–669.[Abstract/Free Full Text]
7. Kumar SD, Emery MJ, Atkins ND, Danta I, Wanner A. Airway mucosal blood flow in bronchial asthma. Am J Respir Crit Care Med 1998;158:153–156.
8. Brieva JL, Danta I, Wanner A. Effect of an inhaled glucocorticosteroid on airway mucosal blood flow in mild asthma. Am J Respir Crit Care Med 2001;161:293–296.[ISI]
9. Kumar SD, Brieva JL, Danta I, Wanner A. Transient effect of inhaled fluticasone on airway mucosal blood flow in subjects with and without asthma. Am J Respir Crit Care Med 2000;161:918–921.[Abstract/Free Full Text]
10. American Thoracic Society. Guidelines for the diagnosis and treatment of patients with chronic pulmonary disease (COPD) and asthma. Am Rev Respir Dis 1987;136:225–244.[ISI][Medline] [Order article via Infotrieve]
11. National Heart, Lung and Blood Institute and National Asthma Education Program. Guidelines for the diagnosis and management of asthma. J Allerg Clin Immunol 1991;88:425–534.[CrossRef][Medline] [Order article via Infotrieve]
12. Crapo RO, Morris AH, Gardner RM. Reference spirometric values using techniques and equipment that meet ATS recommendation. Am Rev Respir Dis 1981;123:659–664.[ISI][Medline] [Order article via Infotrieve]
13. Scuri M, McCaskill V, Chediak AD, Abraham WM, Wanner A. Measurement of airway mucosal blood flow with dimethyl-ether: validation with microspheres. J Appl Phisiol 1995;79:1386–1390.
14. Bousquet J, Ben-Joseph R, Messonnier M, Alemao E, Gould AL. A meta-analysis of the dose response relationship of inhaled corticosteroids in adolescents and adults with mild to moderate persistent asthma. Clin Ther 2002;42:1–20.
15. Holt S, Suder A, Weatherall M, Cheng S, Shirtcliffe P, Beasley R. Dose-response relation of inhaled fluticasone propionate in adolescents and adults with asthma: meta-analysis. BMJ 2001;323:1–8.[Abstract/Free Full Text]
16. Vinod PS, Peck CC, Skelly JP. Vasoconstriction skin-blanching assay for glucocorticoids - a critique. Arch Dermatol 1989;125:1558–1561.[CrossRef][ISI][Medline] [Order article via Infotrieve]
17. Wilson AM, Dempsey OJ, Coutie WRJ, Sims E, Lipworth BJ. Importance of drug-device interaction in determining systemic effects of inhaled corticosteroids. Lancet 1999;353:2128.[CrossRef][ISI][Medline] [Order article via Infotrieve]
18. Kamada A, Szefler SJ, Martin RJ, Boushey HA, Chinchilli VM, Drazen JM. Issues in the use of inhaled corticosteroids. Am J Respir Crit Care Med 1996;153:1739–1748.[ISI][Medline] [Order article via Infotrieve]
19. Barnes PJ, Pedersen S. Efficacy and safety of inhaled corticosteroids in asthma. Am Rev Respir Dis 1993;148:S1–S26.
20. Kelly HW. Establishing a therapeutic index for the inhaled corticosteroids: Pharmacokinetic/pharmacodynamic comparison of inhaled corticosteroids. J Allergy Clin Immunol 1998;102:S36–S51.[CrossRef][ISI][Medline] [Order article via Infotrieve]
21. Martel F, Azevedo I, Oswald W. Extraneural uptake and O-methylation of 3H-adrenaline in rabbit aorta. Naunyn Scmiedebergs Arch Pharmacol 1993;347:363–370.[CrossRef]
22. Horvath G, Lieb T, Conner GE, Salathe M, Wanner A. Steroid sensitivity of norepinephrine uptake by human bronchial arterial and rabbit aortic smooth muscle cells. Am J Respir Cell Mol Biol 2001;25:500–506.[Abstract/Free Full Text]
23. Global Initiative for Asthma. Global strategy for asthma management and prevention NHLBI/WHO workshop report. Publication no. 95-3659Bethesda, National Institutes of Health, National Heart, Lung and Blood Institute, 1995.
24. Kelly WH. Comparison of inhaled corticosteroids. Ann Pharmacother 1998;32:220–232.[Abstract]

This article has been cited by other articles:

				P. Paredi, S. Ward, D. Cramer, P. J. Barnes, and S. A. Kharitonov Normal Bronchial Blood Flow in COPD Is Unaffected by Inhaled Corticosteroids and Correlates With Exhaled Nitric Oxide Chest, April 1, 2007; 131(4): 1075 - 1081. [Abstract] [Full Text] [PDF]

				P. J. Barnes Scientific rationale for using a single inhaler for asthma control Eur. Respir. J., March 1, 2007; 29(3): 587 - 595. [Abstract] [Full Text] [PDF]

				G. J. Rodrigo Rapid effects of inhaled corticosteroids in acute asthma: an evidence-based evaluation. Chest, November 1, 2006; 130(5): 1301 - 1311. [Abstract] [Full Text] [PDF]

				W. ten Hove, L. A. Houben, J. A. M. Raaijmakers, L. Koenderman, and M. Bracke Rapid Selective Priming of Fc{alpha}R on Eosinophils by Corticosteroids J. Immunol., November 1, 2006; 177(9): 6108 - 6114. [Abstract] [Full Text] [PDF]

				A. Wanner, E. S. Mendes, and N. D. Atkins A simplified noninvasive method to measure airway blood flow in humans J Appl Physiol, May 1, 2006; 100(5): 1674 - 1678. [Abstract] [Full Text] [PDF]

				E. S. Mendes, M. A. Campos, and A. Wanner Airway Blood Flow Reactivity in Healthy Smokers and in Ex-Smokers With or Without COPD. Chest, April 1, 2006; 129(4): 893 - 898. [Abstract] [Full Text] [PDF]

				G. Horvath and A. Wanner Inhaled corticosteroids: effects on the airway vasculature in bronchial asthma Eur. Respir. J., January 1, 2006; 27(1): 172 - 187. [Abstract] [Full Text] [PDF]

				G. J. Rodrigo Comparison of Inhaled Fluticasone with Intravenous Hydrocortisone in the Treatment of Adult Acute Asthma Am. J. Respir. Crit. Care Med., June 1, 2005; 171(11): 1231 - 1236. [Abstract] [Full Text] [PDF]

				A. Wanner, G. Horvath, J. L. Brieva, S. D. Kumar, and E. S. Mendes Nongenomic Actions of Glucocorticosteroids on the Airway Vasculature in Asthma Proceedings of the ATS, November 1, 2004; 1(3): 235 - 238. [Abstract] [Full Text] [PDF]

				E. S. Mendes, M. A. Campos, A. Hurtado, and A. Wanner Effect of Montelukast and Fluticasone Propionate on Airway Mucosal Blood Flow in Asthma Am. J. Respir. Crit. Care Med., May 15, 2004; 169(10): 1131 - 1134. [Abstract] [Full Text] [PDF]

				R. Buhl and S. G. Farmer Current and Future Pharmacologic Therapy of Exacerbations in Chronic Obstructive Pulmonary Disease and Asthma Proceedings of the ATS, April 1, 2004; 1(2): 136 - 142. [Abstract] [Full Text] [PDF]

				G. Horvath, Z. Sutto, A. Torbati, G. E. Conner, M. Salathe, and A. Wanner Norepinephrine transport by the extraneuronal monoamine transporter in human bronchial arterial smooth muscle cells Am J Physiol Lung Cell Mol Physiol, October 1, 2003; 285(4): L829 - L837. [Abstract] [Full Text] [PDF]

This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (26) Citing Articles via Google Scholar Google Scholar Articles by Mendes, E.S. Articles by Wanner, A. Search for Related Content PubMed PubMed Citation Articles by Mendes, E.S. Articles by Wanner, A.