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Lipid-laden macrophage index and inflammation in bronchoalveolar lavage fluids in children

Depts of ¹ Pediatrics and ¹ Pathology, University of North Carolina at Chapel Hill, USA

CORRESPONDENCE: M.Y. Kazachkov, Dept of Pediatrics, University of North Carolina at Chapel Hill, 635 Burnett - Womack Building, CB #7220, Chapel Hill, NC, 27599-7220, USA. Fax: 1 9199666179

Keywords: airway inflammation, bronchoalveolar lavage, children, lipid-laden macrophages

Received: May 25, 2000
Accepted June 20, 2001

M.Y. Kazachkov was supported by NIH grant no. 5-;53970-XXXX-;0-;401-4228. This work was also supported in part by the University of North Carolina Center for Environmental Medicine and Lung Biology.

	Abstract

TOP Abstract Materials and methods Results Discussion Acknowledgements References

 
The presence of lipids in alveolar macrophages has been used clinically as an indicator of aspiration, a process associated with increased lung inflammation in animal models. The hypothesis is that the quantity of lipids in alveolar macrophages, measured as lipid-laden index (LLI), would correlate with lung inflammation in paediatric patients.

Children with chronic respiratory symptoms (21 cystic fibrosis (CF), 24 non-CF) underwent flexible bronchoscopy with bronchoalveolar lavage (BAL) and 24-;h intra-oesophageal pH monitoring for clinical indications. Total cell counts, number and per cent of neutrophils and macrophages, and LLI were determined in the bronchoalveolar lavage fluids (BALF) from all children. BALF were also obtained from eight healthy, young nonsmoking adults for comparison.

LLI in non-CF children were 6.9±3.5 (mean±sem) which were higher than LLI in healthy adults (1.0±0.4), (p=0.045). Children with CF had very high LLIs (19.2±4.5) compared with both healthy adults (p=0.014) and children without CF (p=0.045). LLI did not correlate with airway inflammation in any group. LLI in children with abnormal pH probes had a tendency to be higher than in children with normal pH probes, but the difference was not significant (p=0.098).

It is concluded that the lipid-laden index was significantly elevated in children with chronic respiratory symptoms compared with healthy adults, and in children with cystic fibrosis compared with those who have other chronic respiratory conditions. However, the lipid-laden index did not correlate with the quantity of bronchoalveolar lavage fluid inflammation. The lipid-laden index in children may, in part, reflect processes other than aspiration, such as airways obstruction.

Gastro-oesophageal reflux and aspiration may contribute to the development of chronic respiratory diseases, such as asthma, aspiration pneumonitis, chronic bronchitis, bronchiectasis, and a variety of otolaryngological disorders in paediatric patients 1–4. Accumulation of lipids in the cytoplasm of alveolar macrophages is considered to be evidence of aspiration 5, 6. The amount of lipids in alveolar macrophages can be quantitated and expressed as the lipid-laden index (LLI) 7, 8. This index takes into account both the number of macrophages containing lipids, and the relative amount of lipids present in the macrophages.

In animal studies, experimental aspiration of gastric acid and food particles causes intensive inflammation in airways with accumulation of inflammatory cells in the airway lumen and submucosal layer 9, 10. The degree of tissue response is related to the pH and volume of the injected acid solution 9; aspiration of gastric acid with particles of food enhances the inflammatory response 11.

The hypothesis of the present study was that the LLI would correlate with lung inflammation in children. In order to test this hypothesis, inflammation was compared with the LLI in the bronchoalveolar lavage fluids (BALF) of paediatric patients with chronic respiratory symptoms.

	Materials and methods

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Patients
Children with respiratory problems, who underwent clinically indicated flexible bronchoscopy and intra-oesophageal pH monitoring between January 1995–May 1999, were eligible for participation in this study. Gastro-oesophageal reflux (GER) and/or aspiration were thus suspected clinically as one of the possible causes of respiratory symptoms in all children included in this study. Patients with tuberculosis, acute illnesses, or systemic or inhaled steroid medication use within one month prior to bronchoscopy were not included. Because of the known correlation between airway infection and inflammation 12, all infected patients (defined as >5x10⁴ colony forming units (cfu)·mL^–1 of pathogenic bacteria or positive viral culture in BALF) were excluded from analysis. In addition, eight young, nonsmoking, symptom-free adults underwent flexible bronchoscopy with bronchoalveolar lavage (BAL) and served as a healthy control group for comparison with paediatric patients.

The study was approved by the committee for the protection of the rights of the human subjects at the University of North Carolina (UNC) School of Medicine.

Bronchoscopy and bronchoalveolar lavage
Bronchoscopy and BAL in children were performed according to standard clinical protocol at UNC Hospitals. The location of BAL was at the discretion of the bronchoscopist, but was generally performed in the lung segment most affected by disease, as evidenced by radiographical changes or by visual appearance at bronchoscopy. The bronchoscope was wedged into a bronchus and two to three 10-mL aliquots of buffered normal saline solution were instilled and immediately aspirated through the bronchoscope. In some smaller infants, 5-;mL aliquots were used. The total volume of instilled lavage fluid was 1–3 mL·kg^–1 body weight. An aliquot of the BAL for the present study was taken in a sterile manner. The remainder of the BALF was sent to the hospital laboratory for cytology and cultures, which were ordered by the physician responsible for the patient.

Healthy, nonsmoking adult volunteers underwent fibreoptic bronchoscopy with lavage. None of the subjects had a history of asthma, allergic rhinitis, chronic respiratory disease, or cardiac disease. Subjects were excluded from the study if they had recently had an acute respiratory illness, and were asked to avoid exposure to air pollutants such as tobacco smoke and paint fumes. The fibreoptic bronchoscope was wedged into a segmental bronchus of the lingula. Six aliquots of sterile saline were instilled and immediately aspirated. The first was 20 mL and this fraction was labelled the bronchial sample. The remaining five aliquots were 50 mL each and were labelled the alveolar sample. These were put on ice immediately, with determination of total cell counts, differentials, and LLI taking place later.

Lipid index scoring
The BALF was transferred to 25 mL tubes and centrifuged at 1,200xg for 15 min. The supernatant was discarded and the cells were concentrated into 3–4 drops of fluid. One to two drops of the concentrated cellular suspension was transferred to a centrifuge tube with a slide, and spun at 200xg for 10 min. The cytocentrifuged preparations were then stained for lipids with Sudan 4 (Acros Organics, New Jersey, USA).

Each slide was evaluated for the number of lipid-laden macrophages per 100 consecutive macrophages. A semiquantitative method of LLI calculation was used, which was analogous to that reported by Colombo and Hallberg 8. Preliminary studies suggested that dividing the percentage of lipid opacification of the macrophage into <50% and >50%, rather than into quartiles as reported 8, resulted in less interobserver variability. For the calculation of LLI, macrophages with no visible lipid staining of the cytoplasm were scored "0". Macrophages with <50% of the cytoplasm opacified by lipid were scored "1+". Macrophages with >50% of the cytoplasm opacified by lipid were scored "2+". The LLI was then calculated as follows: LLI=((% 1+ macrophages)x1)+((% 2+ macrophages)x2). Nonquantitative descriptors of lipid droplet size or appearance were not attended. The pathologist who calculated the LLI was not aware of clinical findings or the results of the BAL cultures and cell differentials of the patients.

Microbiology
BALF cultures were carried out according to standard protocol at the UNC Hospital's clinical microbiology laboratory 12. Briefly, BALF specimens were cultured for Gram-positive and Gram-negative bacteria. In addition, cultures and stains for viruses (including cytomegalovirus, respiratory syncytial virus, influenza A and B, parainfluenza 1–3, and adenovirus), fungi, and mycobacteria were carried out when these infections were clinically suspected. Oropharyngeal flora (OPF) in BALF specimens were defined according to standard protocol for the UNC Hospital's Clinical Microbiology Laboratory. These consist of a mixture of resident flora, including Streptococcus viridans, nonhaemolytic streptococci, saprophytic Neisseria spp., diphtheroids, coagulase-negative Staphylococcus spp., micrococci, lactobacilli, haemolytic Haemophilis spp., H. parainfluenzae, Eikenella corrodens, non-Group A beta-haemolytic Streptococcus spp., Bacillus spp., and Stomatococcus spp. Quantitative bacterial cultures were performed on both pathogenic flora and OPF. OPF were not included in the total bacterial cfu·mL^–1 BALF used to define whether subjects had infection.

Bronchoalveolar lavage fluid cell count
The samples were processed immediately in the bronchoscopy laboratory. BALF was gently mixed and the total number of white cells in the native, unfiltered lavage fluid was determined in a haemocytometer. Microscope slides were prepared in a Wescor Cytopro 7620 cytocentrifuge (Logan, UT, USA) by centrifuging 150 µL fluid at 60xg for 4 min. To obtain a cell count of 5x10⁴ cells per slide, some samples had to be further diluted in normal saline lavage fluid before loading onto the cytocentrifuge. Slides were fixed and stained with modified Wright-Giemsa stain (Leukostat, Fisher Diagnostic, Pittsburgh, PA, USA). Two hundred consecutive cells were counted under oil immersion and both the number and per cent of macrophages, neutrophils, epithelial cells, lymphocytes and eosinophils were recorded. Differential cell counts were made without knowledge of the BALF culture results.

Intra-oesophageal pH probe monitoring
Intra-oesophageal pH probe monitoring was also performed according to standard clinical protocol at UNC Hospitals. The oesophageal pH electrode (Synectic Medical Irving, TX, USA) was placed 5 cm above the lower oesophageal sphincter for 18–24 h. Total acid exposure time and percentage of time at pH <4.0 (reflux index or pH index) was recorded. Reflux index >5 was considered to be abnormal for all age groups, in accordance with previous reports 13.

Statistical methods
Data are reported as mean±sem unless otherwise stated. Linear regressions were calculated by the method of least squares, and slopes and elevations of the lines were compared using unpaired t-;tests. The data were analysed using the Mann-Whitney U-;test. Statistical significance was defined as p<0.05 throughout.

	Results

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Lipid-laden index in different groups
BALF from 24 uninfected children without cystic fibrosis (CF), 21 uninfected children with CF, and eight healthy adults were available. Clinical indications for bronchoscopy and bronchoscopic diagnoses are summarized in table 1. LLI in non-CF children was 6.9±3.5 (mean±sem). This was higher than in healthy adults (1.0±0.4) (p=0.045). Children with CF had a high LLI (19.2±4.5) compared with both healthy adults (p=0.014) and children without CF (p=0.045) (fig. 1). However, there was no difference in pH index between children with and without CF.

View larger version (11K): [in this window] [in a new window]   Fig. 1.— Lipid-laden index (LLI) for children with and without cystic fibrosis (CF and non-CF), and healthy adult controls. Each bar represents mean±sem. *: p<0.05 versus healthy adults; #: p<0.05 versus healthy adults and non-CF children.

Lipid-laden index and airway inflammation
As expected, based on previous studies 12, children with CF had higher total cell counts and numbers and per cent of neutrophils in their BALF than children without CF. In both CF and non-CF patients, LLI did not correlate with the total cells or number or per cent of neutrophils in BALF. Similarly, there was no correlation between LLI and number or per cent of macrophages and epithelial cells in both groups.

All healthy adult controls had an LLI 3. Therefore, the children without CF were divided into two groups with regard to their LLI. The first group had normal LLI of 3 (n=16) and the second group had high LLI of >3 (n=8). There was no significant difference in the total number of cells, or number or per cent of neutrophils (figs. 2 and 3) in BALF between these two groups. Furthermore, the number and per cent of macrophages and epithelial cells were not different between the two groups. The same analysis was performed for the group of children with CF and, again, there was no difference in total cells, neutrophils, macrophages, or epithelial cells in BALF between groups of children with an LLI 3 (n=7) and an LLI >3 (n=14). There was no difference in LLI between non-CF children with bronchitis (as reported by bronchologist, n=7) and other non-CF patients. In addition, total cells counts and the number and per cent of neutrophils and macrophages were not different.

View larger version (24K): [in this window] [in a new window]   Fig. 2.— Total cells and neutrophils for children without cystic fibrosis divided in two groups (lipid-laden indices (LLI) 3 versus LLI >3). Each bar represents mean±sem. There is no significant difference between cell counts in two groups. BALF: bronchoalveolar lavage fluid.

View larger version (11K): [in this window] [in a new window]   Fig. 3.— Per cent of neutrophils in bronchoalveolar lavage fluid for children without cystic fibrosis divided into the two groups (lipid-laden indices (LLI) 3 versus LLI >3). Each bar represents mean±sem. There is no significant difference in per cent of neutrophils in two groups.

View larger version (13K): [in this window] [in a new window]   Fig. 4.— Lipid-laden indices (LLI) for children without cystic fibrosis with pH index >5, pH index 5 and healthy adults. Each bar represents mean±sem. *: p<0.05 versus healthy adults, and p=0.098 versus children with pH index <5.

	Discussion

TOP Abstract Materials and methods Results Discussion Acknowledgements References

 
This study has found that the LLI in BALF macrophages is elevated in children with chronic respiratory symptoms undergoing clinically indicated bronchoscopy, compared to healthy adult volunteers. However, little relationship was observed between LLI and inflammation in BALF. Animal studies have shown that aspiration of gastric contents containing food particles leads to development of an acute inflammatory response in the airways, mediated by neutrophils 9, 11, 14–16. The severity of airway inflammation correlated with volume and pH of gastric contents in one of these reports 9. De-epithelialization of bronchial mucosa has also been shown to occur 17. In rabbits, Ishii et al. 10 showed that the number and per cent of neutrophils in BALF increased during the first day after aspiration of acid, and subsequently decreased over the next 6 days, while the number and per cent of macrophages were increasing.

The method of calculating LLI used in this study was analogous, but not identical, to that described by Colombo and Hallberg 8. This method was devised to increase confidence in the accuracy of the data; it is often difficult to differentiate the percentage of lipid opacification of macrophage cytoplasm into quartiles. Since a primary goal of this study was the correlation of LLI with quantitative measures of inflammation in BALF, accurate quantification of LLI was essential. However, using a different LLI scoring method makes direct comparison of these data with other studies difficult.

For ethical reasons, the use of healthy children as a control group for LLI was not permitted. Thus, it is conceivable that comparison with adult controls affected the conclusion that LLI are generally elevated in children with chronic respiratory symptoms, since the LLI in healthy children was not determined.

The LLI in children with abnormal reflux was significantly higher than the LLI in healthy adults, and had a tendency to be higher than the LLI in children with normal reflux, although the latter was not statistically significant (p=0.098). This suggests that GER and aspiration may contribute to the accumulation of lipids in alveolar macrophages. This is also consistent with the conclusion of Colombo and Hallberg 18, who found only modest and not statistically significant elevation of LLI after microaspiration of milk in rabbits, which was explained by clearance of lipids from alveolar macrophages after aspiration. It is possible that food particles do not reach alveoli during microaspiration and are evacuated from the respiratory system due to ciliary and cough clearance.

As previously stated, the LLI is used as an indicator of aspiration in BALF from humans. It was therefore hypothesized that the quantity of BALF inflammatory cells would correlate with LLI in paediatric patients with chronic respiratory symptoms. However, the data from young children failed to show any correlation between LLI and BALF cellularity. Although it is possible that pulmonary symptoms in children who aspirate are due to noninflammatory events, such as triggering of receptors in large airways, it might be that increased LLI is not completely specific for aspiration in children with chronic respiratory symptoms. This conclusion is consistent with the recent report by Knauer-Fischer and Ratjen 19, which suggests that elevated lipid indices can be found in a variety of paediatric pulmonary diseases, independent of aspiration.

It is known that factors other than GER and aspiration can cause accumulation of lipids in alveolar macrophages. For example, alveolar macrophages degrade surfactant and surfactant-like lipids, and by-products of surfactant metabolism can be detected in their cytoplasm 20. A significant amount of lipid was found in alveolar macrophages of children with acute chest syndrome due to sickle cell anaemia, and was attributed to pulmonary fat embolism 21. The lipid indices in this group of patients were significantly higher than in a group of patients with aspiration pneumonia. There are also iatrogenic causes of the accumulation of lipids in alveolar macrophages, such as use of the anti-arrhythmic drug amiodarone, a powerful inhibitor of lysosomal phospholipases 22, and treatment with intravenous fat emulsions 23. Finally, both mineral oil aspiration 24 and lipidoses with pulmonary involvement 25 can be associated with increased LLI.

A notable finding of the present study was that in children with CF, the LLI was increased compared with both children without CF and healthy adults, even though the results of their pH measurement studies were not different from children without CF. This suggests that aspiration was not the most important factor leading to accumulation of increased amounts of lipids in alveolar macrophages in children with CF. One possible explanation for this phenomenon could be that prominent inflammation in the airways of children with CF leads to the accumulation of breakdown products from lipid-containing membranes of inflammatory cells in alveolar macrophages. However, the absence of any correlation of LLI with airway inflammation in CF children in this study makes this explanation less satisfactory. It can also be speculated that children with CF have prominent airway obstruction and may develop conditions similar to lipoid pneumonia, which was described in patients with lung cancer 26. It is possible that this process also occurs in children with chronic obstructive pulmonary diseases other than CF.

In summary, it is concluded that the lipid-laden index did not correlate with markers of inflammation in bronchoalveolar lavage fluids in children with chronic respiratory symptoms. Furthermore, the lipid-laden index is elevated in bronchoalveolar lavage fluids from children with cystic fibrosis, who do not appear to have greater gastro-oesophageal reflux or clinical aspiration than other children with chronic respiratory symptoms. The lipid-laden index may lack the specificity needed to establish a diagnosis of aspiration in paediatric patients. Further investigation is required to differentiate the multiple potential sources of lipid accumulation in alveolar macrophages.

	Acknowledgements

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The authors thank I. Kazachkova for technical assistance, A. Ghio for assistance with obtaining bronchoalveolar lavage fluids in healthy adults, and the staff of the University of North Carolina Pediatric Bronchology Center for assistance with procedures.

	References

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