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1 Dept of Respiratory Medicine and Allergy, University Hospital, Umeå, Sweden and 2 Institute for Working Life, Dept of Occupational Chemistry, Umeå, Sweden
CORRESPONDENCE: E. Ädelroth, Dept of Respiratory Medicine and Allergy, University Hospital, S-90185, Umeå, Sweden. Fax: 46 90141369
Keywords: asthma, airway responsiveness, diesel exhaust, induced sputum
Received: September 7, 2000
Accepted January 18, 2001
This work was supported by the Swedish Heart and Lung Foundation.
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Abstract |
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Fourteen nonsmoking, atopic asthmatics with stable disease, on continuous treatment with inhaled corticosteroids, were included. All were hyperresponsive to methacholine. Each subject was exposed to DE (particles with a 50% cut-off aerodynamic diameter of 10 µm (PM10) 300 µg·m–3) and air during 1 h on two separate occasions. Lung function was measured before and immediately after the exposures. Sputum induction was performed 6 h, and methacholine inhalation test 24 h, after each exposure.
Exposure to DE was associated with a significant increase in the degree of hyperresponsiveness, as compared to after air, of 0.97 doubling concentrations at 24 h after exposure (p<0.001). DE also induced a significant increase in airway resistance (p=0.004) and in sputum levels of interleukin (IL)-6 (p=0.048). No changes were detected in sputum levels of methyl-histamine, eosinophil cationic protein, myeloperoxidase and IL-8.
This study indicated that short-term exposure to diesel exhaust, equal to high ambient levels of particulate matter, is associated with adverse effects in asthmatic airways, even in the presence of inhaled corticosteroid therapy. The increase in airway responsiveness may provide an important link to epidemiological findings of exacerbations of asthma following exposure to particulate matter.
During the last decade, several epidemiological studies have provided convincing evidence for an association between ambient levels of particulate matter and various health outcome indices, including respiratory symptoms 1, impaired lung function 2, exacerbations of asthma 3, 4, emergency room visits 1, 5, and an increased risk of respiratory illness in children 6. Importantly, several studies have suggested that individuals with pre-existing respiratory disease such as asthma are more sensitive to elevated ground levels of particulate matter (PM) pollution as compared to healthy subjects 7, 8. Although the epidemiological data linking PM to various health effects is strong and consistent, the knowledge about underlying biological mechanisms, as well as the causality of the observed associations, is still limited.
Diesel exhaust (DE) is a major source of particulate matter 9. Studies conducted in London have shown that the contribution from DE particles to the concentration of particles with a 50% cut-off aerodynamic diameter of 10 µm value (PM10) can account for as much as 87% of the particulate emission from vehicles 10. The particles consist of a carbonaceous core to which various components including acids, aldehydes, hydrocarbons and transition metals can be absorbed. DE particles have further been shown to absorb antigens on to their surface, and thereby potentially increase the deposition of allergens in the respiratory tract 11.
Previously, knowledge about the biological effects of DE was predominantly based on animal studies. However, in recent years, a series of experimental studies has been conducted to evaluate the effects of DE in humans. Using the same exposure system as in the present study, short-term exposure to DE has been shown to induce an acute inflammatory response in human airways, with cellular and cytokine changes detected in sputum, bronchoalveolar lavage and bronchial biopsies 2, 12–15. In addition, nasal instillation of DE particles has been shown to increase immunoglobulin-E in nasal lavage from atopic subjects, and further, to skew cytokine production towards a Th2 type phenotype when instillation of DE particles were combined with allergen challenge 16. Altogether, experimental data have suggested a proinflammatory and allergy-enhancing effect of DE.
In spite of the epidemiological data identifying asthmatics as a sensitive group to PM, the number of controlled exposure studies investigating this issue is still limited. This study hypothesized that the mechanisms by which PM increases respiratory morbidity may involve several components of the asthmatic disease. The aim of the present study was, therefore, to elucidate the effects of short-term exposure to DE in asthmatic airways by specifically addressing the effects on: 1) airway hyperresponsiveness; 2) lung function; and; 3) airway inflammation.
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Methods |
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TOPAbstractMethodsResultsDiscussionAcknowledgementsReferences |
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. All subjects were atopic with a positive skin-prick test to at least one airborne allergen (Soluprick SQ®, ALK, Denmark). The asthmatics were hyperresponsive to methacholine (provocative concentration causing a 20% fall in forced expiratory volume in one second (FEV1) (PC1). All subjects had controlled, stable asthma and were free of respiratory tract infection for 
6 weeks prior to and during the study period. The subjects used inhaled short acting ß2-agonists as needed, as well as daily medication with inhaled corticosteroids (400–1200 µg). Bronchodilators were withheld for at least 8 h before each procedure, whereas the daily required doses of inhaled corticosteroids were continued. As part of the screening procedure for participation in the study, all subjects were tested for their ability to produce sputum on induction with hypertonic saline. The study was conducted during the winter to avoid pollen season. Verbal and written informed consent was obtained from all individuals and the study was approved by the Local Ethics Committee.
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Exposure
The exposure system used in this study has been carefully evaluated and validated 17. All exposures were performed in an environmental chamber according to a previously described standard protocol 2, 12, 13, 17. DE was generated by an idling Volvo diesel engine (Volvo TD45, 4.5 L, 4 cylinders, model 1991, 680 revs·min–1). The concentrations of particles (number·cm–3), oxides of nitrogen (NO2, NO), carbon monoxide and hydrocarbons (HCs) were continuously recorded in the exposure chamber as previously described 13. A Miran 1-A, an infrared-instrument (Foxboro Co., East Bridgewater, MA, USA), was used for analysis of CO. Oxides of nitrogen were analysed with a chemiluminescence instrument, (ECO-Physics CLD 700, Boo Instruments, Stockholm, Sweden). HCs were analysed with an FID-instrument, model 3–300 (JUM Engineering GmbH, Munich, Germany). Mass of particles (µg·m–3) was determined by weighing PM collecting filters. Approximately 90% of the exhaust was shunted away and the remaining part diluted with air and fed into the chamber at a steady state concentration. Air in the chamber was repeatedly changed every 2–3 min, with the waste air being extracted through a tube in the ceiling. The DE entering the environmental chamber was standardized to give a particle concentration of 300 µg·m–3, associated with a median steady state NO2 concentration of 1.2 ppm. Mean concentrations of NO, HCs and CO were 3.4 ppm, 2.6 ppm and 9.1 ppm, respectively. The temperature was maintained at 20°C.
Methacholine inhalation test
The methacholine inhalation test was performed in accordance with the method described by Juniper et al. 18. The test aerosols were generated continuously by a Wright's nebulizer (Roxon Medi-Tech, Montreal, PQ, Canada), delivered into a Hans Rudolph two-way valve (type 1410B, Somedic sales AB, Farsta, Sweden) and inhaled through a mouthpiece by quiet tidal breathing for 2 min with the nose clipped. The nebulizer output was 0.13 mL·min–1. Normal saline was inhaled first, followed by doubling concentrations of methacholine (0.06–16 mg·mL–1) at intervals of 5 min. The response was measured by FEV1 before and at 30, 90 and 180 s after each inhalation. The inhalations were discontinued when there was a fall in FEV1 of 20% or more below the lowest post saline value. Results were expressed as PC20 obtained from the log dose-response curve by linear interpolation of the 2 last points expressed in noncumulative units. The variability of the method is ±one doubling concentration 19.
Lung function test
Lung function responses were measured using a computerized whole body plethysmograph (system 2800, Sensor Medics Corp., CA, USA). Forced vital capacity (FVC), FEV1 and airway resistance (Raw) were measured immediately before and after each exposure.
Sputum induction
Sputum induction was performed by a method slightly modified from that described by Pin et al. 20. Briefly, hypertonic saline (3%) was nebulized using an ultrasonic nebulizer (DeVilbiss 2000, DeVilbiss Co., Somerset, PA, USA) with an output of 1.5 mL·min–1. All subjects received an inhaled ß2-agonist (0.5 mg terbutaline) before the induction. Inhalation was conducted at 3 intervals of 7 min. FEV1 was monitored before commencing and after every inhalation period. Following each inhalation interval, subjects were advised to rinse their mouth with water and blow their nose before trying to cough sputum into a sterile plastic container. The obtained samples were kept on ice up to 1 h prior to processing.
Sputum processing
Sputum was processed according to the method described by Pizzichini et al. 21. Briefly, portions appearing more viscid and dense were selected from the expectorated samples and transferred to a 10 mL siliconized tube. After adding 0.1% diothiotreitol (DTT) at a volume equal to four times the selected sputum weight, the sputum was rocked for 15 min to dissolve the mucus and disperse the cells. Phosphate buffered saline (PBS) was then added at a volume equal to that of DTT and the rocking continued for 5 min. The mixture was filtered through a 48 µm mesh nylon filter into another 10 mL tube and centrifuged at 300xg for 10 min at 4°C. The supernatant was then separated from the cell pellet, re-centrifuged at 1,000xg for 10 min to further remove debris, aspirated and stored in Eppendorf tubes at –70°C for later analyses. The cell pellet was resuspended in 1,000 µL PBS and total cell count and cell viability were determined in a haemocytometer using trypan blue. Cell suspension was adjusted to 0.5x106 cells·mL–1 and 50 µL were placed in each cup of a Shandon 3 cytocentrifuge (Shandon Southern Instruments Inc., Sewikly, PA, USA). Cytospins were made on pre-wet slides, prepared at 400 rpm for 5 min and stained with May-Grünwald-Giemsa. At least 400 nonsquamous cells were counted and the differential cell counts were expressed as a percentage of the total nonsquamous cell count. The proportion of squamous cells was obtained by counting 400 additional cells and expressing this as a percentage of the total number of cells. Samples were considered adequate for analysis if the squamous cell contamination was <20% and the viability >50%. Total cell count was calculated by dividing the total number of cells by the volume of processed sputum (l mg=1 µL).
Fluid phase measurements
The sputum supernatant samples were analysed forsix different soluble proteins, including interleukin (IL)-6, IL-8, IL-10, methyl-histamine, eosinophil cationic protein (ECP) and myeloperoxidase (MPO). IL-6, IL-8 and IL-10 were measured with commercial enzyme linked immunosorbent assay kits (R&D Systems Inc., Abingdon, UK). Levels of methyl-histamine, ECP and MPO were determined using sensitive commercial radioimmunoassay kits (Pharmacia & Upjohn, JAB, Uppsala, Sweden).
Statistical Analysis
Wilcoxon's nonparametric signed-ranks test for paired observations was used to compare sputum data on cells and soluble markers after air and diesel exhaust exposures. Comparison of lung function data was performed with the paired samples t-test. All methacholine values were log-transformed before statistical analysis. Results are presented as geometric mean±geometric standard error of the mean (SEM). The comparison between methacholine values after air and diesel exposure was done with the t-test. A p-value<0.05 was considered significant.
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Results |
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TOPAbstractMethodsResultsDiscussionAcknowledgementsReferences |
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. The degree of hyperresponsiveness was significantly increased 24 h after exposure to DE as compared to after air, with the mean methacholine PC20 following diesel and air being 1.77±1.35 mg·mL–1 and 3.47±1.36 mg·mL–1, respectively (p<0.001). The mean diesel versus air shift in the PC20 was 0.97 doubling concentrations. In addition, the increase in airway responsiveness following diesel exhaust was confirmed by comparing the data from the methacholine tests performed 3–7 days before the exposures with the postexposure data. A significant difference was seen between the prediesel data (PC20 2.79±1.25 mg·mL–1) and the postdiesel exposure data (PC20 1.77±1.35 mg·mL–1; p=0.005). As expected, no change was seen between the pre-air (PC20 2.53±1.25 mg·mL–1) and post-air exposures (PC20 3.47±1.36 mg·mL–1). The change in PC20 (prediesel minus postdiesel versus pre-air minus postair) was also significant (p=0.001).
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. Raw data is based on 13 subjects, since one subject was not able to complete the Raw measurement due to technical difficulties. Following exposure to DE, a significant increase in airway resistance was induced compared with after air ((Raw air minus pre-air)/pre-air versus (Raw postdiesel minus prediesel)/prediesel), p=0.004 (fig. 2). No changes were detected in FVC or FEV1 values between exposures.
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). A significant increase was seen in the fluid phase concentration of IL-6 after exposure to DE compared with after air, whereas no significant changes were detected in the levels of IL-8, ECP, MPO or methyl-histamine (table 3).
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Discussion |
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Airway hyperresponsiveness is an important feature of asthma and has been shown to correlate well with the severity of the disease 22. In the present study, methacholine data obtained at 24 h postair exposure was compared with the postdiesel data, and it was found that exposure to DE induced an increase in airway responsiveness equal to a shift in PC20 methacholine of almost one doubling concentration. The relevance of this finding is supported by the consistency of the data, with 12 of 14 subjects showing decreased PC20 values following DE exposure, as well as by a high level of statistical significance. The increase in airway responsiveness was further confirmed by analysis of the change from pre-exposure to postexposure revealing a significant increase following exposure to DE. In concordance with the epidemiological data suggesting a 1–4 day lag effect for most health outcomes to PM 4, the increase in airway responsiveness was detected 24 h after exposure. This finding indicates a long lasting response to DE in asthmatic airways. Importantly, the late increase in airway responsiveness presented here is of particular interest, as a prolonged response may not only be hazardous by itself, but might also increase the time when other stimuli can trigger asthma symptoms.
DE was associated with a significant increase in IL-6, as recently shown in healthy subjects at the same timepoint after DE exposure 2. The increase in IL-6 probably represents an acute response to DE and may result from direct effects of DE on airway epithelial cells. IL-6 is a multifunctional cytokine that exerts a number of pro-inflammatory effects, including induction of acute-phase reactions and regulation of immunological responses. Interestingly, exposure to DE particles has previously been associated with a release of IL-6 from human bronchial epithelial cells 23. No changes were seen in ECP, MPO, methyl-histamine and IL-8. However, the cytokines all showed a large variability, which makes the interpretation of data difficult.
In healthy subjects, DE has recently been shown to induce a significant increase in sputum neutrophil percentages, together with elevated levels of methyl-histamine at 6 h following exposure 2. In the present study, no changes were seen in these parameters, suggesting a different response in asthmatics as compared to healthy subjects. The apparent lack of a detectable cellular inflammation to DE presented here may be explained by several different factors. Firstly, all subjects were on continuous medication with inhaled corticosteroids, which may have prevented cellular influx by inhibition of neutrophil chemotactic factors and may also have suppressed mast cell degranulation, explaining the lack of a response in histamine. Secondly, the time for developing an inflammatory reaction to DE may differ between asthmatics and healthy subjects. The underlying inflammation seen in asthmatic airways may be associated with compensatory and counter-regulatory mechanisms that act to dampen or delay inflammatory changes to various stimuli. Research aimed at clarifying differences between asthmatics and healthy subjects in the response to DE is of great interest as it may provide a link to the epidemiological findings that have identified asthmatics as a sensitive subpopulation to PM pollution.
The biological mechanisms by which DE may induce hyperresponsiveness are still to be clarified. Recent animal studies have demonstrated that DE exposure, when combined with allergen challenges, enhances airway responsiveness and causes airway inflammation characterized by an increase in airway eosinophils, neutrophils, mast cells and cytokines 24–27. Interestingly, DE alone also enhanced airway responsiveness, but did not induce any inflammatory changes 24, 26. The increase in airway responsiveness seen in the present study was only associated with mild inflammatory changes in sputum. One obvious explanation to this mismatch is the fact that sputum inflammatory changes were investigated 6 h after exposure, whereas airway responsiveness was measured at 24 h, thus reflecting different timepoints after exposure. However, it is possible that the increase in hyperresponsiveness to DE may represent a nonspecific irritative reaction, rather than a specific inflammatory response.
Furthermore, it is not known whether it is the particulate or gaseous phase of DE that is responsible for the increase in airway responsiveness presented here. Animal studies have provided evidence that the particulate fraction of DE is able to cause an increase in airway responsiveness 28, but to the best of the authors' knowledge, there are no previous experimental data concerning particulate pollution and hyperresponsiveness in humans. Regarding the gaseous phase of DE, some studies have reported an immediate increase in airway responsiveness following exposure to NO2 29, 30, whereas other studies have not 31. Most studies have been designed to explore the immediate and early effects of NO2 and it is not clear from the literature whether NO2 is able to induce a sustained increase in airway responsiveness.
During the last decade there has been a burst of epidemiological studies investigating the health effects of particulate air pollution. Most studies have focused on addressing the effects of short-term exposure to PM and there is convincing epidemiological evidence to suggest an association between increased levels of PM and adverse health effects on both respiratory and cardiovascular morbidity and mortality 4, 32. Most studies did not observe a particulate pollution threshold. Instead, the relative risk of morbidity and mortality has generally been shown to increase in a near-linear fashion with increased particulate concentrations 33. In the present study, subjects were exposed to DE at a concentration of 300 µg·m–3 PM10, which represents a high ambient concentration encountered in busy streets of major cities, as well as in certain occupational settings. In a global perspective, more than half of the world's megacities (cities with a population>10 million) have annual average levels of particulate matter ranging 200–600 µg·m–3 34.
In conclusion, the findings of this study suggest that exposure to diesel exhaust is associated with adverse effects in asthmatic subjects, including an increase in airway responsiveness, airway resistance and in the release of interleukin-6 following a short-term exposure to diesel exhaust. Importantly, these changes were observed in spite of the fact that all subjects had stable asthma and were on regular medication with inhalation steroids. The increase in airway responsiveness presented here may provide an important link to epidemiological findings of exacerbations of asthma following exposure to particulate matter peaks.
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: before exposure; 
: after exposure. The thick horizontal lines represent median values, boxes represent 25th–75th percentile range and the extended bars represent the largest and smallest observed values within 1.5 box lengths.