ERJ
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Permissions
Right arrowRequest Permissions
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wirtz, H.
Right arrow Articles by Schmidt, M
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wirtz, H.
Right arrow Articles by Schmidt, M
Eur Respir J 1996; 9: 24-32
Copyright © ERS Journals Ltd 1996

Original Articles

Acute influence of cigarette smoke on secretion of pulmonary surfactant in rat alveolar type II cells in culture

HR Wirtz and M Schmidt

It has been shown, that smoking results in a lower yield of surfactant associated phospholipids in bronchoalveolar lavage (BAL). Indirect evidence suggests impaired secretion. In the present study, we investigated the influence of cigarette smoke on surfactant secretion in cultured rat alveolar type II cells. Smoke exposure was achieved by bubbling the smoke of four cigarettes through Dulbecco's modified Eagle's medium (DMEM) which was adjusted to a reference absorption value of 1.36 at 320 nm. Cells were preincubated with various dilutions of cigarette smoke-treated medium for 30 min, and were then exposed to this medium for 2 h. After this time, secretion of 3H-choline-labelled phosphatidylcholine (PC) was measured as a marker of surfactant secretion. A 10 fold dilution of cigarette smoke-treated medium inhibited PC secretion stimulated by a combination of terbutaline, adenosine triphosphate and 12-O-tetradecanoylphorbol-13-acetate by over 50%, but did not alter basal secretion. Exposure to less concentrated cigarette smoke-treated medium resulted in less inhibition. Cellular injury was not observed with the concentrations of cigarette smoke-treated medium used in this study. The gas phase of cigarette smoke was not inhibitory at comparable concentrations. Longer exposure to cigarette smoke-treated medium resulted in increased inhibition of PC secretion. The cigarette smoke ingredients, nicotine and benzo[a]pyrene, failed to inhibit PC secretion. Secretion of type II cells exposed to cigarette smoke-treated medium at lower temperatures was not affected. Addition of antioxidants to medium and cells during the preincubation and secretion period did not alter cigarette smoke-treated medium-induced inhibition of stimulated PC secretion. These results demonstrate a direct inhibitory effect of cigarette smoke constituents on surfactant secretion in type II cells. Inhibition is mediated by compounds contained predominantly in the particulate phase of cigarette smoke. Inactivation of the inhibitory effect by lower temperatures suggests involvement of processes such as enzymatic bioactivation or active transport mechanisms.


This article has been cited by other articles:


Home page
 Am. J. Respir. Cell Mol. Bio.Home page
F. Facchinetti, F. Amadei, P. Geppetti, F. Tarantini, C. Di Serio, A. Dragotto, P. M. Gigli, S. Catinella, M. Civelli, and R. Patacchini
{alpha},beta-Unsaturated Aldehydes in Cigarette Smoke Release Inflammatory Mediators from Human Macrophages
Am. J. Respir. Cell Mol. Biol., November 1, 2007; 37(5): 617 - 623.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
H van der Vaart, D S Postma, W Timens, and N H T Ten Hacken
Acute effects of cigarette smoke on inflammation and oxidative stress: a review
Thorax, August 1, 2004; 59(8): 713 - 721.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. Hammerschmidt, H. Kuhn, T. Grasenack, C. Gessner, and H. Wirtz
Apoptosis and Necrosis Induced by Cyclic Mechanical Stretching in Alveolar Type II Cells
Am. J. Respir. Cell Mol. Biol., March 1, 2004; 30(3): 396 - 402.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
S V Culpitt, D F Rogers, P S Fenwick, P Shah, C De Matos, R E K Russell, P J Barnes, and L E Donnelly
Inhibition by red wine extract, resveratrol, of cytokine release by alveolar macrophages in COPD
Thorax, November 1, 2003; 58(11): 942 - 946.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
S. V. Culpitt, D. F. Rogers, P. Shah, C. De Matos, R. E. K. Russell, L. E. Donnelly, and P. J. Barnes
Impaired Inhibition by Dexamethasone of Cytokine Release by Alveolar Macrophages from Patients with Chronic Obstructive Pulmonary Disease
Am. J. Respir. Crit. Care Med., January 1, 2003; 167(1): 24 - 31.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
R. E. K. Russell, S. V. Culpitt, C. DeMatos, L. Donnelly, M. Smith, J. Wiggins, and P. J. Barnes
Release and Activity of Matrix Metalloproteinase-9 and Tissue Inhibitor of Metalloproteinase-1 by Alveolar Macrophages from Patients with Chronic Obstructive Pulmonary Disease
Am. J. Respir. Cell Mol. Biol., May 1, 2002; 26(5): 602 - 609.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1996 by the European Respiratory Society.