|
|
||||||||
Original Articles |
A small population of T-cells does not express the conventional T-cell receptor (TCR), characterized by the alpha and beta polypeptide chains (alpha/beta TCR) but two polypeptides termed gamma and delta (gamma/delta TCR). Changes in gamma/delta TCR expression may be relevant as the cause or consequence of several diseases. Our study was undertaken to determine and compare the distribution of T-cells expressing gamma/delta TCR in blood and bronchoalveolar lavage (BAL) of patients with sarcoidosis, hypersensitivity pneumonitis (HP), idiopathic pulmonary fibrosis (IPF), and of healthy controls. In addition, the association between gamma/delta TCR of blood T-lymphocytes and accessory molecules was analysed. Using direct immunofluorescence with the anti-gamma/delta TCR and anti-CD3 monoclonal antibodies (MoAbs) followed by flow cytometric analysis, the blood of patients with pulmonary sarcoidosis, HP, IPF and of healthy controls was analysed. To reveal the association between gamma/delta TCR of blood T-lymphocytes and the accessory molecules, expression of CD4, CD8 and CD25 were determined. Calculating the percentage and the total number of CD3+ gamma/delta TCR cells in blood, the data indicated a significant increase of gamma/delta T-cells in individuals with pulmonary sarcoidosis and HP, compared to healthy controls and IPF patients. In sarcoidosis patients with elevated CD3+ gamma/delta TCR levels, significantly lower CD4/CD8 ratios were observed. In addition, our data demonstrate a correlation between the decrease of CD4+ cells in blood and the amplified appearance of gamma/delta TCR expression in sarcoidosis patients, but not in HP patients.(ABSTRACT TRUNCATED AT 250 WORDS)
This article has been cited by other articles:
![]() |
G. de Laurentiis, L. Vitiello, L. Racioppi, F. Perna, M. Galgani, G. Merola, P. Carratu, M. Maniscalco, S. Marsico, and M. Sofia CD8+ T-cell alveolitis in familial pulmonary alveolar microlithiasis Eur. Respir. J., July 1, 2007; 30(1): 165 - 171. [Abstract] [Full Text] [PDF] |
||||
![]() |
P. L. Simonian, C. L. Roark, F. Diaz del Valle, B. E. Palmer, I. S. Douglas, K. Ikuta, W. K. Born, R. L. O'Brien, and A. P. Fontenot Regulatory Role of {gamma}{delta} T Cells in the Recruitment of CD4+ and CD8+ T Cells to Lung and Subsequent Pulmonary Fibrosis J. Immunol., October 1, 2006; 177(7): 4436 - 4443. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. V. Wisnewski, H. Cain, N. Magoski, H. Wang, C. T. Holm, and C. A. Redlich Human gamma /delta T-Cell Lines Derived from Airway Biopsies Am. J. Respir. Cell Mol. Biol., March 1, 2001; 24(3): 332 - 338. [Abstract] [Full Text] [PDF] |
||||
![]() |
G. S. Davis, L. M. Pfeiffer, and D. R. Hemenway Interferon-gamma Production by Specific Lung Lymphocyte Phenotypes in Silicosis in Mice Am. J. Respir. Cell Mol. Biol., April 1, 2000; 22(4): 491 - 501. [Abstract] [Full Text] |
||||
![]() |
R. J. Harbeck Clin. Vaccine Immunol., May 1, 1998; 5(3): 271 - 277. [Full Text] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |