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Copyright ©ERS Journals Ltd 2007
Quantitative scoring of an interferon-
assay for differentiating active from latent tuberculosis
Divisions of 1 Pulmonary Diseases, 2 Immunology and Allergy, and 3 Quality of Care, Geneva University Hospital, Geneva, Switzerland.
CORRESPONDENCE: J-P. Janssens, Centre antituberculeux, Hôpital Cantonal Universitaire, 1211 Geneva 14, Switzerland. Fax: 41 223729929. E-mail: Jean-Paul.Janssens{at}hcuge.ch
Keywords: Interferon-
release assays, latent tuberculosis infection, tuberculosis
Received: March 11, 2007
Accepted May 14, 2007
The aim of this study was to assess the contribution of an interferon-
The investigation was a prospective study of contacts exposed to a case of contagious TB and cases of HIV-negative culture-proven TB referred over a 16-month period. Tuberculin skin tests (TSTs) and T-SPOT.TB were performed in 310 contacts 8–12 weeks after exposure. In subjects with culture-proven TB, T-SPOT.TB was performed within 2 weeks of initiation of treatment. The analysis included all contacts with a positive T-SPOT.TB result and all subjects with TB.
TB contacts (n = 127) and cases (n = 58) were included. Mean±SD T-SPOT.TB results were 107±56 (range 1–207) sfu for TB, 54±60 (7–239) sfu for contacts with positive T-SPOT.TB results and a TST induration diameter of >5 mm, and 19±27 (7–143) sfu for contacts with positive T-SPOT.TB results and a TST induration diameter of
Although T-SPOT.TB results were significantly related to disease activity, the test cannot be recommended for the diagnosis of tuberculosis.
release assay (T-SPOT.TB) to the differentiation of active tuberculosis (TB) from latent TB infection by quantifying spot-forming units (sfu). 
5 mm. By receiver operating characteristic curve analysis, a threshold value of 49.5 sfu showed a sensitivity of 83% and specificity of 74% for distinguishing latent TB infection from TB.
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