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8-Isoprostane as a marker of oxidative stress in nonsymptomatic cigarette smokers and COPD

¹ Dept of Medicine and Divisions of Pulmonary Medicine, ² Clinical Physiology, and ³ Allergology, University of Helsinki and Helsinki University Central Hospital, Helsinki, Finland.

CORRESPONDENCE: V. L. Kinnula, Dept of Medicine, University of Helsinki, P.O. Box 22, 00014 Helsinki, Finland. Fax: 358 947176107. E-mail: vuokko.kinnula{at}helsinki.fi

Keywords: Chronic obstructive pulmonary disease, cigarette smoking, oxidant, sputum

Received: February 14, 2006
Accepted September 19, 2006

8-Isoprostane is a potential in vivo marker for oxidant burden, but its usefulness in induced sputum of smokers and chronic obstructive pulmonary disease (COPD) has not been investigated.

The current study investigated 58 subjects comprising 11 never-smokers, 11 ex-smokers, 13 healthy current smokers and 23 COPD with stage 0–III disease (according to the Global Initiative for Chronic Obstructive Lung Disease criteria). 8-Isoprostane was determined from induced sputum by enzyme immunoassay.

Sputum 8-isoprostane levels were similar in the never-smokers and ex-smokers, but were elevated in the healthy smokers compared with nonsmokers, and in those with stage I–III COPD. Sputum 8-isoprostane levels could not differentiate nonsymptomatic smokers from those with Stage 0 COPD. There was a correlation between sputum 8-isoprostane level and lung function parameters (forced expiratory volume in one second/forced vital capacity and sputum neutrophils.

In conclusion, sputum 8-isoprostane levels correlate with the severity of chronic obstructive pulmonary disease. However, they do not appear to differentiate healthy smokers from those who are at risk of developing chronic obstructive pulmonary disease (Global Initiative for Chronic Obstructive Lung Disease stage 0).

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