HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via ISI Web of Science (1) Citing Articles via Google Scholar Google Scholar Articles by Sjåheim, T. Articles by Halstensen, T. S. Search for Related Content PubMed PubMed Citation Articles by Sjåheim, T. Articles by Halstensen, T. S.

Reduced bronchial CD4+ T-cell density in smokers with occupational asthma

¹ Laboratory for Mucosal Immunology (LMI), Dept of Oral Biology, University of Oslo, ² Dept of Respiratory Medicine, Rikshospitalet, Oslo, ³ Health Dept, Hydro Aluminium, Karmøy, Håvik, and ⁴ Health Dept, Elkem Aluminium, Lista, Farsund, Norway.

CORRESPONDENCE: T. Sjåheim, Rikshospitalet, 0027 Oslo, Norway. Fax: 47 23073917. E-mail: tonebs{at}odont.uio.no

Keywords: Asthma, inflammation, smoking, T-lymphocyte subsets

Received: March 7, 2006
Accepted July 26, 2006

Cigarette smoking may alter bronchial inflammation in asthma. Multicolour immunohistofluorescent examination on bronchial cryosections was used to examine bronchial inflammatory cell infiltrate in patients with occupational asthma. Monoclonal antibodies to CD3, CD4, CD8, T-cell receptor-1, CD68 and human leukocyte antigen-DR were combined to identify T-cell subsets and macrophages in bronchial biopsies from 20 workers with occupational asthma (12 smokers and eight nonsmokers), 15 healthy workers (seven smokers and eight nonsmokers) and 10 nonsmoking, nonexposed controls.

The increased subepithelial CD4+ T-cell density in nonsmoking asthmatics was not present in smoking asthmatics, who had the lowest CD4+ T-cell density of all groups. The decreased subepithelial CD4+ and CD8+ T-cell density correlated with a reduction in lung function, as measured by percentage predicted forced expiratory volume in one second, in smoking asthmatics only. Although smoking asthmatics had a significantly increased number of intraepithelial CD8+ T-cells and macrophages compared with nonsmoking asthmatics, the proportion of -T-cells was significantly decreased in both asthmatic groups.

Smoking asthmatics had a distinctly different distribution of T-cell subsets compared with nonsmoking asthmatics. The accumulation of subepithelial CD4+ T-cells, which was observed in nonsmoking asthmatics, appeared to be inhibited in smoking asthmatics, suggesting a smoking-induced bronchial immune modulation, at least in occupational asthma in the aluminium industry.