HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (4) Citing Articles via Google Scholar Google Scholar Articles by Van daele, S. Articles by De Baets, F. Search for Related Content PubMed PubMed Citation Articles by Van daele, S. Articles by De Baets, F.

Survey of Pseudomonas aeruginosa genotypes in colonised cystic fibrosis patients

¹ Cystic Fibrosis Centre and ² Microbiology Department, University Hospital, Ghent, ³ Cystic Fibrosis Centre, University Hospital, Leuven, ⁴ Cystic Fibrosis Centre, HUDERF-Erasme Hospital (ULB), ⁵ Cystic Fibrosis Centre, University Hospital of the Free University of Brussels, Brussels, ⁶ Cystic Fibrosis Centre, Hospital of the Catholic University of Louvain, Louvain-la-Neuve, ⁷ Cystic Fibrosis Centre, University Hospital Liège, Liège, ⁸ Cystic Fibrosis Centre, Sint-Vincentius Hospital, and ⁹ University Hospital Antwerp, Antwerp, Belgium.

CORRESPONDENCE: S. Van daele, CF-centre, University Hospital Ghent, 5K6, De Pintelaan 185, 9000 Gent, Belgium. Fax: 32 92403861. E-mail: Sabine.Vandaele{at}ugent.be

Keywords: Cystic fibrosis, epidemiology, genotyping, national survey, Pseudomonas aeruginosa

Received: January 9, 2006
Accepted May 12, 2006

The current authors aimed to examine whether cystic fibrosis (CF) patients in Belgium shared Pseudomonas aeruginosa genotypes and to compare the genotypes of isolates from the same patients during two consecutive years. A Belgian databank of the P. aeruginosa genotypes of all colonised CF patients was created.

Sputum samples from a total of 276 P. aeruginosa colonised patients during 2003, and from a subgroup of 95 patients in 2004, were analysed. Patients were asked about any social contact between each other by questionnaire. All P. aeruginosa isolates exhibiting different colonial morphology on McConkey agar were first genotyped using arbitrarily primed PCR, whereafter single representatives of each randomly amplified polymorphic DNA-type were further genotyped by fluorescent amplified fragment length polymorphism analysis.

In the 213 patients from whom P. aeruginosa could be cultured (resulting in 910 isolates), a total of 163 genotypes were found. The majority (75%) of patients harboured only one genotype. In most of the limited number of clusters, previous contacts between patients could be suspected. In 80% of the patients studied during both years, P. aeruginosa genotype remained unchanged.

In conclusion, most colonised cystic fibrosis patients harbour only one Pseudomonas aeruginosa genotype, despite showing different colonial morphotypes. The number of clusters is limited, and most patients seem to retain the same genotypic strain during both years.

This article has been cited by other articles:

				P. Schelstraete, S. Van daele, K. De Boeck, M. Proesmans, P. Lebecque, J. Leclercq-Foucart, A. Malfroot, M. Vaneechoutte, and F. De Baets Pseudomonas aeruginosa in the home environment of newly infected cystic fibrosis patients Eur. Respir. J., April 1, 2008; 31(4): 822 - 829. [Abstract] [Full Text] [PDF]

				H. Vu-Thien, G. Corbineau, K. Hormigos, B. Fauroux, H. Corvol, A. Clement, G. Vergnaud, and C. Pourcel Multiple-Locus Variable-Number Tandem-Repeat Analysis for Longitudinal Survey of Sources of Pseudomonas aeruginosa Infection in Cystic Fibrosis Patients J. Clin. Microbiol., October 1, 2007; 45(10): 3175 - 3183. [Abstract] [Full Text] [PDF]