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Eur Respir J 2005; 25:1077-1083
Copyright ©ERS Journals Ltd 2005

Involvement of retinoic acid-inducible gene-I in the IFN-{gamma}/STAT1 signalling pathway in BEAS-2B cells

T. Imaizumi1, M. Kumagai1, K. Taima2, T. Fujita3, H. Yoshida1 and K. Satoh1

1 Depts of Vascular Biology, Institute of Brain Science, and 2 Internal Medicine, Hirosaki University School of Medicine, Hirosaki, and 3 Dept of Tumour Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan

CORRESPONDENCE: T. Imaizumi, Dept of Vascular Biology, Institute of Brain Science, Hirosaki University School of Medicine, 5-Zaifu-cho, Hirosaki 036-8562, Japan. Fax: 81 172395135. E-mail: timaizum@cc.hirosaki-u.ac.jp

Keywords: BEAS-2B, interferon-{gamma}, interferon-{gamma}-inducible protein 10, retinoic acid-inducible gene-I, signal transducer and activator of transcription 1

Received: September 1, 2004
Accepted February 9, 2005

Bronchial epithelial cells play an important role in airway host defence, and interferon (IFN)-{gamma} controls immune reactions by regulating the expression of various genes in bronchial epithelial cells. Signal transducer and activator of transcription 1 (STAT1) is the key transcriptional factor in IFN-{gamma} signalling. Retinoic acid-inducible gene-I (RIG-I) is a member of the DExH box family of proteins and designated a putative RNA helicase. RNA helicases play diverse roles in regulation of gene expression and cellular functions, and RIG-I is implicated in antiviral responses.

The aim of the present study was to investigate the effect of IFN-{gamma} on RIG-I expression in a cell line derived from human bronchial epithelial cells, BEAS-2B.

Induction of RIG-I in response to IFN-{gamma} was found in BEAS-2B cells. Induction of RIG-I by IFN-{gamma} was also demonstrated in another pulmonary epithelial cell line, NCI-H292. Transfection of BEAS-2B cells with RIG-I complementary DNA resulted in the upregulation of STAT1. Induction of IFN-{gamma}-inducible protein 10 by IFN-{gamma} was enhanced in the cells overexpressing RIG-I.

It is concluded that retinoic acid-inducible gene-I may play an important role in the regulation of immunological reactions in bronchial epithelial cells elicited by interferon-{gamma}.






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Copyright © 2005 by the European Respiratory Society.