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Stretch-induced changes in constricted lung parenchymal strips: role of extracellular matrix

¹ Respiratory Unit, Salvatore Maugeri Foundation, Research Institute, Cassano Murge (BA), Italy and ² Meakins-Christie Laboratories, McGill University, Montreal, QC, Canada

CORRESPONDENCE: M.S. Ludwig, Meakins-Christie Laboratories, McGill University, 3626 St Urbain Street, Montreal, QC, Canada, H2X 2P2. Fax: 1 5143987483. E-mail: mara.ludwig@mcgill.ca

Keywords: complex impedance, contractile elements, tissue mechanics

Received: March 6, 2003
Accepted September 15, 2003

This work was supported by the J.T. Costello Memorial Research Fund and the Canadian Institutes of Health Research.

Abstract

Large amplitude oscillations of contracted airway smooth muscle cause relative relaxation of the preparation. However, little is known about the effect of mechanical stretch on distal lung behaviour.

Rat parenchymal strips were suspended in an organ bath and attached at one end to a force transducer and at the other end to a servo-controlled lever arm that effected length changes. Mechanical impedance of the strip was measured by applying a complex signal consisting of pseudorandom length oscillations of varying frequencies (0.5–19.75 Hz). A constant phase model was fit to changes in length and tension to calculate tissue damping (G) and elastance (H). Hysteresivity was calculated as G/H. Impedance was measured before and after sinusoidal length oscillation at different amplitudes (1, 3, 10 and 25% of resting length) at a frequency of 1 Hz under baseline conditions and after acetylcholine-induced constriction.

Oscillations of 10 and 25% amplitudes significantly decreased the G and H of the lung strip. The effect of length oscillations was no different in control versus constricted strips.

These data suggest that in the distal lung, large stretches affect the structural components of the extracellular matrix rather than the contractile elements.

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