| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 University Children's Hospital and 2 Dept of Pathology, University of Würzburg, Würzburg, Germany
CORRESPONDENCE: C.P. Speer, University Children's Hospital, Würzburg, Josef-Schneider-Strasse 2, 97080, Würzburg, Germany. Fax: 49 931 20127833. E-mail: speer_c@klinik.uni-wuerzburg.de
Keywords: cell death, hyperoxia, pulmonary surfactant, signal transduction
Received: April 7, 2003
Accepted July 29, 2003
Apoptosis and proliferation and the effect of exogenous surfactant on these processes were investigated in the lungs of mechanically ventilated/oxygen-treated preterm infants with respiratory distress syndrome and stillborn foetuses.
Apoptotic and proliferation indices were determined in lung tissue sections from 27 ventilated/oxygen-treated preterm infants and 29 stillborn foetuses. The effect of exogenous surfactant on apoptosis and proliferation was studied in 16 ventilated preterm infants; 11 untreated infants served as control. Apoptotic and proliferating cells were identified by double labelling combining terminal deoxynucleotidyltransferase-mediated deoxyuridine triphosphate nick end-labelling or Ki-67 with cell marker proteins. Pathways to cell death were explored by immunolabelling of cleaved caspases-3, -8 and -9.
In the lungs of ventilated/oxygen-treated preterm infants, the numbers of apoptotic and proliferating cells increased significantly compared to the respective numbers in the lungs of stillborn foetuses. Apoptosis was detected in alveolar epithelial cells, whereas epithelial, endothelial and smooth muscle cells proliferated. Surfactant treatment reduced apoptosis induced by ventilation/oxygen-treatment; however, the decrease was not significant. Caspases-8 and -9 do not contribute to ventilation-induced apoptosis, whereas caspase-3 is involved.
In conclusion, ventilation/oxygen-treatment induces epithelial cell apoptosis and proliferation of epithelial, endothelial and smooth muscle cells in the lungs of preterm infants.
This article has been cited by other articles:
|
|
 |
|
  M. E. De Paepe, S. Gundavarapu, U. Tantravahi, J. R. Pepperell, S. A. Haley, F. I. Luks, and Q. Mao Fas-Ligand-Induced Apoptosis of Respiratory Epithelial Cells Causes Disruption of Postcanalicular Alveolar Development Am. J. Pathol., July 1, 2008; 173(1): 42 - 56. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-S. Lee, Y. Wang, B. S. Maciejewski, K. Esho, C. Fulton, S. Sharma, and J. Sanchez-Esteban Interleukin-10 protects cultured fetal rat type II epithelial cells from injury induced by mechanical stretch Am J Physiol Lung Cell Mol Physiol, February 1, 2008; 294(2): L225 - L232. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-R. Tang, G. Seedorf, V. Balasubramaniam, A. Maxey, N. Markham, and S. H. Abman Early inhaled nitric oxide treatment decreases apoptosis of endothelial cells in neonatal rat lungs after vascular endothelial growth factor inhibition Am J Physiol Lung Cell Mol Physiol, November 1, 2007; 293(5): L1271 - L1280. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kunzmann, C. P. Speer, A. H. Jobe, and B. W. Kramer Antenatal inflammation induced TGF-beta1 but suppressed CTGF in preterm lungs Am J Physiol Lung Cell Mol Physiol, January 1, 2007; 292(1): L223 - L231. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. De Paepe, Q. Mao, Y. Chao, J. L. Powell, L. P. Rubin, and S. Sharma Hyperoxia-induced apoptosis and Fas/FasL expression in lung epithelial cells Am J Physiol Lung Cell Mol Physiol, October 1, 2005; 289(4): L647 - L659. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
