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1 Dept of Internal Medicine, Pulmonary and Critical Care Medicine and 2 Dept of Pathology, UT Southwestern Medical Center, Dallas, TX, USA
CORRESPONDENCE: Y-S. Yang, Dept of Internal Medicine, Pulmonary and Critical Care Medicine, UT Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390-9034, USA. Fax: 214 6482283. E-mail: Yih-Sheng.Yang@UTSouthwestern.edu
Keywords: BR22, surfactant protein B, thyroid transcription factor-1, thyroid transcription factor-1 associated protein 26
Received: December 18, 2002
Accepted March 14, 2003
This work was supported by the grant R01 HL63525 to Y-S. Yang, the Will Rogers Institute and the James M. Collins Center for Biomedical Research to J.C. Weissler.
The current authors have previously identified BR22, a thyroid transcription factor (TTF)-1 associated protein 26 (TAP26), which interacts with TTF-1 to enhance human surfactant protein (SP)-B promoter activity in transfected 293 cells. However, the expression of TAP26 in the lung cells and its biological relevance to the SP-B production under physiological conditions were not examined.
In this study, endogenous co-immunoprecipitation and in situ immunohistochemical staining techniques were employed to explore the presence of TAP26 and TTF-1 complex in the lung epithelial cells. The correlation of TAP26, TTF-1 and SP-B expression was inspected in H441 cells in the presence of dexamethasone, a known positive effector of the SP-B promoter.
Monoclonal antibody (mAb) against TAP26 can co-immunoprecipitate both TAP26 and TTF-1 from H441 cells. Using this antibody in in situ staining of human lung sections, the data show that TAP26 is present in the lung alveolar epithelial cells. Reverse transcriptase-polymerase chain reaction and Western blot analyses of type-II cells as well as dexamethasone-treated H441 cells suggest that TAP26 expression is modulated coordinately with SP-B and TTF-1 in these cells.
In summary, the current study demonstrates that thyroid transcription factor-1 associated protein 26 is an associated protein of thyroid transcription factor-1 in the lung alveolar epithelial cells where surfactant protein gene expressions take place in vivo.
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