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1 Dept of Medicine, Karolinska Institute, Division of Respiratory Medicine, Lung Research Laboratory, Karolinska Hospital, Stockholm, and 2 Amersham Biosciences and 3 Ludwig Institute for Cancer Research, Uppsala, Sweden
CORRESPONDENCE: F. Sabounchi-Schütt, Karolinska Institutet, Dept of Medicine, Division of Respiratory Medicine, Lung Research Laboratory, L4:0, Karolinska Hospital, SE-171 76, Stockholm, Sweden
Keywords: bronchoalveolar lavage fluid, matrix-assisted laser desorption/ionisation time of flight mass spectrometry, protein quantitation, protein-spot pattern, sarcoidosis, two-dimensional polyacrylamide gel electrophoresis
Received: July 9, 2002
Accepted November 29, 2002
This study was supported by the Swedish Medical Research Council (K2002-74X-14182-01A), The Swedish Heart-Lung Foundation, the King Oscar II Jubilee Foundation and Karolinska Institutet.
In sarcoidosis, an inflammatory lung disease, the protein profile of bronchoalveolar lavage fluid (BALF) is altered. To study the BALF protein pattern changes in sarcoidosis, samples from six patients and four healthy individuals were analysed by two-dimensional polyacrylamide gel electrophoresis.
A comparison of the protein-spot patterns showed a significantly higher number of protein spots in the pH range 5.5–6.7 in patients compared to controls (472 versus 384). Furthermore, the number of protein spots in the patients were significantly decreased in the acidic pH range 4.5–5.5 (399 versus 518). Measurement of the optical density in the gels showed varying expression levels for several protein spots. Seventeen of the altered protein spots were identified, of which seven have previously not been reported for BALF. Many of these are nonplasma proteins involved in the inflammatory and oxidant-antioxidant processes.
In conclusion, the bronchoalveolar lavage fluid protein content is altered in sarcoidosis patients, especially for proteins that are not derived from plasma. The described proteomics approach will in the future be used to asses overall changes in the protein content associated with sarcoidosis and may offer the possibility of identifying disease-specific proteins.
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