|
|
||||||||
Depts of 1 Pharmacology and 4 Physiology, Faculty of Medicine, University of Valencia, and 2 Dept of Medical Bioanalysis, Institute of Biomedical Research of Barcelona, Spanish Council for Scientific Research, Barcelona, Spain. 3 Zambon Group SpA, Bresso, Italy
CORRESPONDENCE: E.J. Morcillo, Dept of Pharmacology, Faculty of Medicine, 15 Av. Blasco Ibáñez, E-46010, Valencia, Spain. Fax: 34 963864622. E-mail: esteban.morcillo@uv.es
Keywords: N-Acetylcysteine, airway inflammation, experimental asthma, inducible nitric oxide synthase, nuclear transcription factor-
B
Received: May 14, 2002
Accepted October 8, 2002
S. Blesa, M. Mata and A. Serrano were supported by the Ministry of Science and Technology (Madrid, Spain) and J. Suchankova by a North Atlantic Treaty Organisation (Brussels, Belgium) grant. This study was supported by grants from the European Union and Spanish Ministry of Science and Technology (1FD97-1143, SAF1999-0111 and SAF2000-0144) and a research grant from Zambon Group SpA (Bresso, Italy).
Oxidative stress is involved in the pathophysiology of inflammatory airway diseases including asthma; therefore, antioxidants might be of clinical benefit in asthma treatment. In the present study, the effects of N-acetylcysteine on sensitised brown Norway rats were examined.
N-Acetylcysteine (3 mmol·kg body weight1 administered orally) was given daily for 1 week before challenge and various antigen-induced pulmonary responses were studied.
Antigen exposure increased lipid peroxidation in bronchoalveolar lavage fluid (BALF) and oxidised glutathione levels in lung tissue 2 h after challenge. Lung nuclear transcription factor-
These results indicate that oral N-acetylcysteine exerts an antioxidant protective effect and attenuates pulmonary inflammation in experimental asthma.
B-binding activity was increased 2 h after challenge, and BALF tumour necrosis factor-
and inducible nitric oxide synthase expression in lungs peaked 4 h after challenge. Expression of intercellular adhesion molecule-1 and mucin MUC5AC was also increased 4 h after challenge. These changes in oxidant status, transcription factor activation, and inflammatory cytokine and gene expression were reduced by N-acetylcysteine. This thiol did not affect the immediate bronchospasm reaction to antigen in anaesthetised rats but inhibited airways hyperresponsiveness to 5-hydroxytryptamine and the augmented eosinophil numbers in BALF, which appear 24 h after exposure of conscious rats to antigen aerosol, and abolished antigen-induced extravasation of Evans blue into BALF.
This article has been cited by other articles:
![]() |
M. Martinez-Losa, J. Cortijo, G. Juan, M. Ramon, M. J. Sanz, and E. J. Morcillo Modulatory effects of N-acetyl-L-cysteine on human eosinophil apoptosis Eur. Respir. J., September 1, 2007; 30(3): 436 - 442. [Abstract] [Full Text] [PDF] |
||||
![]() |
R. Demiralay, N. Gursan, and H. Erdem Regulation of nicotine-induced apoptosis of pulmonary artery endothelial cells by treatment of N-acetylcysteine and vitamin E Human and Experimental Toxicology, July 1, 2007; 26(7): 595 - 602. [Abstract] [PDF] |
||||
![]() |
A. Buenestado, J. Cortijo, M.-J. Sanz, Y. Naim-Abu-Nabah, M. Martinez-Losa, M. Mata, A. C. Issekutz, E. Marti-Bonmati, and E. J. Morcillo Olive Oil-Based Lipid Emulsion's Neutral Effects on Neutrophil Functions and Leukocyte-Endothelial Cell Interactions JPEN J Parenter Enteral Nutr, July 1, 2006; 30(4): 286 - 296. [Abstract] [Full Text] [PDF] |
||||
![]() |
H. Yasuda, M. Yamaya, T. Sasaki, D. Inoue, K. Nakayama, M. Yamada, M. Asada, M. Yoshida, T. Suzuki, H. Nishimura, et al. Carbocisteine inhibits rhinovirus infection in human tracheal epithelial cells Eur. Respir. J., July 1, 2006; 28(1): 51 - 58. [Abstract] [Full Text] [PDF] |
||||
![]() |
T. Rangasamy, J. Guo, W. A. Mitzner, J. Roman, A. Singh, A. D. Fryer, M. Yamamoto, T. W. Kensler, R. M. Tuder, S. N. Georas, et al. Disruption of Nrf2 enhances susceptibility to severe airway inflammation and asthma in mice J. Exp. Med., July 5, 2005; 202(1): 47 - 59. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. Alvarez, M. Cerda-Nicolas, Y. Naim Abu Nabah, M. Mata, A. C. Issekutz, J. Panes, R. R. Lobb, and M.-J. Sanz Direct evidence of leukocyte adhesion in arterioles by angiotensin II Blood, July 15, 2004; 104(2): 402 - 408. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. Almolki, C. Taille, G. F. Martin, P. J. Jose, C. Zedda, M. Conti, J. Megret, D. Henin, M. Aubier, and J. Boczkowski Heme oxygenase attenuates allergen-induced airway inflammation and hyperreactivity in guinea pigs Am J Physiol Lung Cell Mol Physiol, July 1, 2004; 287(1): L26 - L34. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. Mata, A. Ruiz, M. Cerda, M. Martinez-Losa, J. Cortijo, F. Santangelo, A. Serrano-Mollar, A. Llombart-Bosch, and E.J. Morcillo Oral N-acetylcysteine reduces bleomycin-induced lung damage and mucin Muc5ac expression in rats Eur. Respir. J., December 1, 2003; 22(6): 900 - 905. [Abstract] [Full Text] [PDF] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |