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Regulation of E-cadherin expression by dexamethasone and tumour necrosis factor- in nasal epithelium

¹ Institut National Scientifique et de la Recherche Médicale U454, Clinique des Maladies Respiratoires, Centre Hospitalier Universitaire-Montpellier, Hôpital Arnaud de Villeneuve and ² Service Othoryno-Laryngology, Centre Hospitalier Universitaire-Montpellier, Hôpital Gui de Chauliac, Montpellier, France

CORRESPONDENCE: P. Chanez, Clinique des Maladies Respiratoires, Hôpital Arnaud de Villeneuve, 371 Av. du Doyen Gaston Giraud, 34295, Montpellier Cedex 5, France. Fax: 33 467521848. E-mail: chanez@montp.inserm.fr

Keywords: cadherin, catenin, glucocorticoids, inflammation, nasal epithelium

Received: February 18, 2002
Accepted July 25, 2002

Asthma and rhinitis often coexist and share many clinical features. The extent of epithelial alteration in nasal inflammation is controversial. Cell-cell adhesion plays an important role in tissue morphogenesis and homeostasis and is mediated by the cadherin family. In human bronchial epithelial cells the authors have shown that tumour necrosis factor (TNF)- induced a significant decrease of E-cadherin and ß-catenin expression. The addition of dexamethasone inhibited this decrease. The aim of the present study was to investigate the effect of TNF- and dexamethasone on the regulation of E-cadherin, -catenin and ß-catenin in human nasal epithelial cells (HNEC).

A primary culture of HNEC, obtained from human nasal turbinates after surgery, was used. The quantitative and qualitative modulation of E-cadherin, -catenin and ß-catenin expression was assessed by Western blot and immunofluorescence analysis. In order to assess the TNF--induced activation of HNEC, interleukin-8 and RANTES (regulated on activation, normal T-cell expressed and secreted) release was assessed by enzyme-linked immunosorbent assay.

The results showed that TNF- induced, a decrease in -catenin and ß-catenin expression, but had no effect on E-cadherin expression. Immunofluorescence showed that TNF- induced cytoplasmic localisation of E-cadherin, -catenin and ß-catenin. Dexamethasone inhibited the effect of TNF- and induced a three-fold increase in E-cadherin expression.

These results suggest that the difference in nasal and bronchial epithelial cohesion may be due to the differential effect of tumour necrosis factor- and dexamethasone on E-cadherin expression.

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