ERJ
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (18)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Petrek, M.
Right arrow Articles by du Bois, R.M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Petrek, M.
Right arrow Articles by du Bois, R.M.
Eur Respir J 2002; 20:1206-1212
Copyright ©ERS Journals Ltd 2002

CC and C chemokine expression in pulmonary sarcoidosis

M. Petrek1, V. Kolek2, J. Szotkowská1 and R.M. du Bois3

1 Depts of Immunology and 2 Respiratory Medicine, Palacky University and University Hospital, Olomouc, Czech Republic. 3 Interstitial Lung Disease Unit, Royal Brompton Hospital, London, UK

CORRESPONDENCE: M. Petrek, Dept of Immunology, Palacky University, IP Pavlova str. 6, 775 20 Olomouc, Czech Republic. Fax: 420 585415116. E-mail: petrekm@fnol.cz

Keywords: CC chemokine ligand, lung, lymphotactin, monocyte chemoattractant protein-1, RANTES, XC chemokine ligand

Received: October 26, 2001
Accepted June 16, 2002

This study was supported by the Czech Ministries of Health (IGA 3768-3) and of Schools, Education and Physical Training (MSM 151100002).

The chemokines RANTES (regulated on activation, T-cell expressed and secreted; CC chemokine ligand (CCL)-5) and monocyte inflammatory protein (MIP)-1{alpha} (CCL-3) have been implicated in the development of alveolitis in pulmonary sarcoidosis. The novel C chemokine single cysteine motif (SCM)-1{alpha} (XCL-1) and the CC chemokine monocyte chemoattractant protein (MCP)-1 (CCL-2) are also mononuclear-cell attractants and represent alternative candidate mediators of alveolitis. Therefore, the expression of MCP-1 and SCM-1{alpha} was investigated together with the expression of RANTES and MIP-1{alpha} in bronchoalveolar lavage fluid (BALF) from control subjects and patients with sarcoidosis. The relationship between chemokine expression and sarcoidosis clinical course was also explored.

Messenger ribonucleic acid (mRNA) expression for all four chemokines was determined by semiquantitative reverse transcriptase-polymerase chain reaction of RNA extracted from unseparated bronchoalveolar cells (17 patients, 12 controls). RANTES, MIP-1{alpha} and MCP-1 proteins were measured by enzyme-linked immunosobent assay of unconcentrated BALF (60 patients, 17 controls).

MCP-1, and namely RANTES and SCM-1{alpha} mRNA expression was upregulated in sarcoidosis, particularly in patients with more advanced disease. RANTES, and namely MCP-1 concentrations were elevated in BALF samples obtained from patients; MCP-1 levels were most increased in patients with chest radiographic stage 2 disease and also in patients with persistent and recurrent disease.

In conclusion, chemokines monocyte chemotactive protein-1 and single cysteine motif-1{alpha} are, in addition to RANTES, associated with the development of alveolitis in sarcoidosis and their expression parallels the disease course.




This article has been cited by other articles:


Home page
 Am. J. Respir. Crit. Care Med.Home page
E. Kriegova, C. Melle, V. Kolek, B. Hutyrova, F. Mrazek, A. Bleul, R. M. du Bois, F. von Eggeling, and M. Petrek
Protein Profiles of Bronchoalveolar Lavage Fluid from Patients with Pulmonary Sarcoidosis
Am. J. Respir. Crit. Care Med., May 15, 2006; 173(10): 1145 - 1154.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
M. Takeuchi, K. Oh-i, J. Suzuki, T. Hattori, A. Takeuchi, Y. Okunuki, Y. Usui, and M. Usui
Elevated Serum Levels of CXCL9/Monokine Induced by Interferon-{gamma} and CXCL10/Interferon-{gamma}-Inducible Protein-10 in Ocular Sarcoidosis.
Invest. Ophthalmol. Vis. Sci., March 1, 2006; 47(3): 1063 - 1068.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
W. Matsuyama, H. Mitsuyama, M. Watanabe, Y. Shirahama, I. Higashimoto, M. Osame, and K. Arimura
Involvement of Discoidin Domain Receptor 1 in the Deterioration of Pulmonary Sarcoidosis
Am. J. Respir. Cell Mol. Biol., December 1, 2005; 33(6): 565 - 573.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
D. A. Culver, B. P. Barna, B. Raychaudhuri, T. L. Bonfield, S. Abraham, A. Malur, C. F. Farver, M. S. Kavuru, and M. J. Thomassen
Peroxisome Proliferator-Activated Receptor {gamma} Activity Is Deficient in Alveolar Macrophages in Pulmonary Sarcoidosis
Am. J. Respir. Cell Mol. Biol., January 1, 2004; 30(1): 1 - 5.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
A. Gibejova, F. Mrazek, D. Subrtova, V. Sekerova, J. Szotkowska, V. Kolek, R. M. du Bois, and M. Petrek
Expression of Macrophage Inflammatory Protein-3{beta}/CCL19 in Pulmonary Sarcoidosis
Am. J. Respir. Crit. Care Med., June 15, 2003; 167(12): 1695 - 1703.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2002 by the European Respiratory Society.