HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Permissions Request Permissions Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (5) Citing Articles via Google Scholar Google Scholar Articles by Sadeghi-Hashjin, G. Articles by Folkerts, G. Search for Related Content PubMed PubMed Citation Articles by Sadeghi-Hashjin, G. Articles by Folkerts, G.

Sodium cromoglycate and doxantrazole are oxygen radical scavengers

Dept of Pharmacology and Pathophysiology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, the Netherlands

CORRESPONDENCE: G. Sadeghi-Hashjin, Dept of Veterinary Basic Sciences, Urmia University, PO Box 641, Urmia, Iran. Fax: 98 4413443442. E-mail: g.sadeghi@mail.urmia.ac.ir

Keywords: alveolar macrophages, chemiluminescence, doxantrazole, oxygen radicals, sodium cromoglycate

Received: January 19, 2002
Accepted May 9, 2002

The effects of two mast cell stabilisers, sodium cromoglycate (SCG) and doxantrazole, on the formation of reactive oxygen species (ROS) were studied. Guinea-pig alveolar macrophages (AMs) generated lucigenin-dependent chemiluminescence (LDCL). This was increased when the cells were stimulated by phorbol myristate acetate (PMA) or zymosan (by 133% and 464%, respectively, in total LDCL over 60 min). SCG decreased PMA-induced LDCL at higher concentrations (10 mM, by 55%) than doxantrazole (1 mM, by 75%). SCG decreased radical production by AMs in response to zymosan in a concentration-dependent manner by 72%. Doxantrazole (0.1–1 mM) diminished total LDCL by 30–80%. In addition, glucose oxidase led to LDCL generation when incubated with glucose in a cell-free medium. This was inhibited by 47–83% in the presence of SCG or doxantrazole. SCG and doxantrazole inhibited the hydrogen peroxide- and peroxynitrite-induced LDCL by 92%. Moreover, these drugs slightly increased the survival rate of the AMs.

It is concluded that doxantrazole- and sodium cromoglycate-inhibited lucigenin-dependent chemiluminescence production by guinea-pig alveolar macrophages is due to a direct scavenging effect on reactive oxygen species. Doxantrazole is 10-times more potent. Mast cell stabilisers may be effective in allergic asthma not only by preventing the allergen-induced mediator release, but also by preventing radical-induced lung damage.

This article has been cited by other articles:

				Z.-Q. Hei, X.-L. Gan, H.-Q. Huang, G.-J. Luo, S.-R. Li, and J. Cai Protective effects of cromolyn sodium on intestinal ischaemia-reperfusion-triggered lung injury in rats Br. J. Anaesth., March 1, 2007; 98(3): 407 - 408. [Full Text] [PDF]

				L. Ferrier, F. Berard, L. Debrauwer, C. Chabo, P. Langella, L. Bueno, and J. Fioramonti Impairment of the Intestinal Barrier by Ethanol Involves Enteric Microflora and Mast Cell Activation in Rodents Am. J. Pathol., April 1, 2006; 168(4): 1148 - 1154. [Abstract] [Full Text] [PDF]