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Copyright ©ERS Journals Ltd 2002
Increased expression of nuclear factor-
B in bronchial biopsies from smokers and patients with COPD
1 Salvatore Maugeri Foundation, IRCCS, Medical Centre of Rehabilitation, Division of Pulmonary Disease, Veruno, Italy. 2 Dept of Thoracic Medicine, National Heart and Lung Institute, Imperial College School of Medicine, London, UK
CORRESPONDENCE: I.M. Adcock, Dept of Thoracic Medicine, National Heart and Lung Institute, Imperial College School of Medicine, Dovehouse Street, London, SW3 6LY, UK. Fax: 44 2073518126. E-mail: ian.adcock@ic.ac.uk
Keywords: airflow limitation, airway inflammation, nuclear factor-
B, T-lymphocytes
Received: August 13, 2001
Accepted March 28, 2002
This study was supported by the Associazione per la Ricerca e la Cura dell'Asma (ARCA, Padua, Italy), Salvatore Maugeri Foundation, IRCCS, "Ricerca Corrente" and Glaxo-Wellcome (UK).
The expression of nuclear factor (NF)-
Bronchial biopsies were obtained from 14 smokers with COPD, 17 smokers with normal lung function and 12 nonsmokers with normal lung function. The number of p65 positive (+) cells was quantified by immunohistochemistry and the expression of p65 in bronchial biopsies from the three groups was examined by Western blotting (WB).
Smokers with normal lung function and patients with COPD had increased numbers of p65+ cells in the epithelium and increased p65 nuclear expression. In COPD patients the number of epithelial p65+ cells correlated with the degree of airflow limitation. WB analysis showed an increase in p65 in smokers with normal lung function and COPD patients (p<0.05).
Bronchial biopsies in smokers with normal lung function and chronic obstructive pulmonary disease patients show increased expression of p65 protein, predominantly in the bronchial epithelium. Disease severity is associated with an increased epithelial expression of nuclear factor-
B is an indicator of cellular activation and of inflammatory mediator production. The aim of the present study was to characterise the expression and localisation of p65, the major subunit of NF-B, in the bronchial mucosa of patients with chronic obstructive pulmonary disease (COPD), and to examine the relationship between p65 expression and disease status. B.
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