HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (4) Citing Articles via Google Scholar Google Scholar Articles by Bals, R. Articles by Jany, B. Search for Related Content PubMed PubMed Citation Articles by Bals, R. Articles by Jany, B.

Identification of disease genes by expression profiling

¹ Medical Dept, Division of Pulmonology, Hospital of the University of Munich, Munich, and ² Missionsärztliche Klinik, Würzburg, Germany

CORRESPONDENCE: R. Bals, Hospital of the University of Munich, Medizinische Klinik und Poliklinik I Großhadern, 81377, Munich, Germany. Fax: 49 8970958877

Keywords: differential display, gene expression, genomics, microarray chip analysis, proteomics

Received: January 5, 2001
Accepted June 12, 2001

Work related to differential gene expression in the authors' laboratory was supported by grants of the Friedrich-Baur-Stiftung (Munich, Germany) and the Deutsche Forschungsgemeinschaft (Ba 1641/3-;1).

Abstract

The human genome has been completely sequenced. The development of innovative methodologies and tools to understand the functions of human genes in health and disease will allow the data of the human genome project to be utilized.

This paper reviews methods that can be used to detect and isolate genes that are specifically expressed in certain diseases or that are specific to cell types. First, classical methods, such as differential screening of complementary deoxyribonucleic acid libraries and subtractive techniques, are described. Methods based on polymerase chain reaction (PCR), such as differential display PCR or serial analysis of gene expression, will then be discussed. Finally, recent developments in gene chip technology and basic principles of functional genomics will be illustrated.

Future developments will link the results of genomic approaches to data obtained by other systematic methods, such as proteomics (i.e. the systematic, large scale analysis of proteins), and will allow the production of a detailed molecular characterization of diseases, disease stages, tissues, or cell types.

Methods to detect disease or cell type-specific gene expression patterns will play an important role in the future of basic research, as well as the development of novel diagnostic procedures and identification of therapeutic targets.

This article has been cited by other articles:

				A. Jahoor, R. Patel, A. Bryan, C. Do, J. Krier, C. Watters, W. Wahli, G. Li, S. C. Williams, and K. P. Rumbaugh Peroxisome Proliferator-Activated Receptors Mediate Host Cell Proinflammatory Responses to Pseudomonas aeruginosa Autoinducer J. Bacteriol., July 1, 2008; 190(13): 4408 - 4415. [Abstract] [Full Text] [PDF]