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Physiomer® reduces the chemokine interleukin-;8 production by activated human respiratory epithelial cells

¹ Inserm U 514, IFR 53, CHU Maison Blanche, Reims Cedex, France. ² Goemar Laboratories, ZAC La Madeleine, Saint-Malo, France

CORRESPONDENCE: J. Jacquot, Inserm Unité 514, IFR 53, Hôpital Maison Blanche, 45 rue Cognacq-Jay, 51092, Reims Cedex, France. Fax: 33 326065861

Keywords: inflammation, interleukin-;8, nuclear factor-;B, respiratory epithelial cells, sodium chloride

Received: August 25, 2000
Accepted March 29, 2001

This work was supported in part by Inserm and by a grant from the Association Vaincre la Mucoviscidosc (no. P0004). The Goemar Laboratories (Saint-Malo, France) fund O. Tabary through an Inserm/Goemar Laboratories collaboration (contract no. 97210).

The authors have recently shown that the transcription factor nuclear factor-B (NF-B) is a central mediator in the NaCl-mediated interleukin (IL)-;8 production by human airway epithelial cells. In this study, it was investigated whether Physiomer®, an isotonic sea water-derived solution commercialized for cleaning the nasal mucosa, impaired the chemokine IL-;8 expression and secretion by human respiratory epithelial cells compared with that obtained with an isotonic 9% NaCl solution.

Primary human bronchial gland (HBG) epithelial cells were incubated either in Physiomer® or in a NaCl 9% solution and activated either with 20 ng·mL^–1 tumour necrosis factor-;, or IL-;1ß, respectively. Physiomer® significantly reduced the IL-;8 protein release in basal and activated HBG cells in comparison with that obtained with the 9% NaCl solution. In contrast to the effects of Physiomer® observed on resting HBG cells, Physiomer® did not significantly reduce the level of phosphorylation of the NF-B inhibitor protein IB or the steady-state IL-;8 messenger ribonucleic acid levels in activated HBG cells, suggesting that Physiomer® would have a post-transcriptional effect on IL-;8 expression in activated HBG cells.

The authors conclude that Physiomer® is potentially useful in the reduction of airway mucosal inflammation.