ERJ
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Permissions
Right arrowRequest Permissions
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (27)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Silvestri, M.
Right arrow Articles by Rossi, G.A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Silvestri, M.
Right arrow Articles by Rossi, G.A.
Eur Respir J 2001; 18:139-145
Copyright ©ERS Journals Ltd 2001


Fluticasone and salmeterol downregulate in vitro, fibroblast proliferation and ICAM-1 or H-CAM expression

M. Silvestri1, L. Fregonese1, F. Sabatini1, G. Dasic2 and G.A. Rossi1

1 Pulmonary Division, G. Gaslini Institute, Genoa, and 2 GlaxoWellcome S.p.A., Verona, Italy

CORRESPONDENCE: G.A. Rossi, Dept. of Pulmonary Diseases, G. Gaslini Institute, Largo G. Gaslini 5, 16147, Genoa, Italy. Fax: 39 0103776590

Keywords: airway remodelling, ß2-agonists, bronchial asthma, corticosteroids

Received: July 27, 2000
Accepted March 6, 2001

ß2-adrenoreceptor agonists have pharmacological properties that may suggest an inhibitory effect on various aspects of the inflammatory and repair processes that characterize asthma.

Since fibroblasts express ß2-adrenoreceptors, the effects of different concentrations (0.1–100 nM) of fluticasone propionate (FP), salmeterol (S) and their combination (FP+S) on lung fibroblast proliferation and adhesion molecule expression were evaluated.

Stimulation of human foetal lung fibroblasts with a fibrogenic cytokine, basic fibroblast growth factor (bFGF), resulted in a [methyl-3H] thymidine ([3H]TdR) uptake, four-fold higher than that of control cultures (p=0.0001) and was significantly inhibited by S, at all the concentrations tested (0.1–100 nM; p<0.05). No changes in bFGF-induced cell proliferation were observed in the presence of FP (0.1–100 nM; p>0.05, all comparisons). In addition, the association FP+S did not improve the inhibitory activity of S alone (p>0.05, each comparison). An upregulation of intercellular adhesion molecule-1 (ICAM-1) expression was induced by tumour necrosis factor-{alpha} (TNF-{alpha}) (p=0.0004), but not by interleukin-4 (IL-4) (p>0.05), while none of the two cytokines were able to increase hyaluronic-cellular adhesion molecule (H-CAM) expression by lung fibroblasts (p>0.05). A significant downregulation of ICAM-1 or H-CAM expression was demonstrated in the presence of FP or S, at all concentrations tested (0.1–100 nM; p<0.01, each comparison). Interestingly, S (10 nM and 100 nM) was able to enhance the inhibitory activity of FP on ICAM-1 expression (p<0.01), but not on H-CAM expression (p>0.1).

These results show that in human foetal lung fibroblasts, fluticasone propionate and salmeterol are effective in modulating in vitro, different lung fibroblast biological functions that are likely to be involved in airway remodelling.




This article has been cited by other articles:


Home page
Eur Respir JHome page
J. E. Ward, T. Harris, T. Bamford, A. Mast, M. C. F. Pain, C. Robertson, D. Smallwood, T. Tran, J. Wilson, and A. G. Stewart
Proliferation is not increased in airway myofibroblasts isolated from asthmatics
Eur. Respir. J., August 1, 2008; 32(2): 362 - 371.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Respir. Cell Mol. Bio.Home page
L. Todorova, E. Gurcan, A. Miller-Larsson, and G. Westergren-Thorsson
Lung Fibroblast Proteoglycan Production Induced by Serum Is Inhibited by Budesonide and Formoterol
Am. J. Respir. Cell Mol. Biol., January 1, 2006; 34(1): 92 - 100.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Respir. Cell Mol. Bio.Home page
T. S. Wilkinson, S. Potter-Perigo, C. Tsoi, L. C. Altman, and T. N. Wight
Pro- and Anti-Inflammatory Factors Cooperate to Control Hyaluronan Synthesis in Lung Fibroblasts
Am. J. Respir. Cell Mol. Biol., July 1, 2004; 31(1): 92 - 99.
[Abstract] [Full Text] [PDF]


Home page
JAMAHome page
D. D. Sin, F. A. McAlister, S. F. P. Man, and N. R. Anthonisen
Contemporary Management of Chronic Obstructive Pulmonary Disease: Scientific Review
JAMA, November 5, 2003; 290(17): 2301 - 2312.
[Abstract] [Full Text] [PDF]


Home page
ThoraxHome page
P A Beckett and P H Howarth
Pharmacotherapy and airway remodelling in asthma?
Thorax, February 1, 2003; 58(2): 163 - 174.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Respir. Crit. Care Med.Home page
N. J. Vanacker, E. Palmans, R. A. Pauwels, and J. C. Kips
Effect of Combining Salmeterol and Fluticasone on the Progression of Airway Remodeling
Am. J. Respir. Crit. Care Med., October 15, 2002; 166(8): 1128 - 1134.
[Abstract] [Full Text] [PDF]


Home page
Eur Respir JHome page
P.J. Barnes
Scientific rationale for inhaled combination therapy with long-acting {beta}2-agonists and corticosteroids
Eur. Respir. J., January 1, 2002; 19(1): 182 - 191.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2001 by the European Respiratory Society.