Eur Respir J 2001; 17:969-974
Copyright ©ERS Journals Ltd 2001
Expression of discoidin domain receptor 1 tyrosine kinase on the human bronchial epithelium
O. Sakamoto1,
M. Suga1,
T. Suda2 and
M. Ando1
1 First Dept of Internal Medicine and 2 Dept of Cell Differentiation, IMEG, Kumamoto University School of Medicine, Kumamoto, Japan
CORRESPONDENCE: O. Sakamoto, First Dept of Internal Medicine, Kumamoto University School of Medicine, 1-1-1 Honjo, Kumamoto, 860-0811, Japan. Fax: 81 963710582
Keywords: bronchial epithelium, discoidin domain receptor 1, receptor tyrosine kinase, type IV collagen
Received: January 12, 2000
Accepted July 14, 2000
This work was supported in part by Grants-in-Aid from the Ministry of Education, Science and Culture of Japan.
Discoidin domain receptor 1 (DDR1) tyrosine kinases constitute a novel family of receptors characterized by a unique structure in the ectodomain (discoidin-I domain). The DDR1 ligand is the extracellular matrix protein collagen.
To identify receptor tyrosine kinases (RTKs) involved in control of growth and differentiation of human bronchial epithelial (HBE) cells, a polymerase chain reaction-based search for RTKs in HBE cells was performed. DDR1 was the most abundant clone identified. Northern analysis detected a 3.6 kb DDR1 messenger ribonucleic acid (mRNA) expressed in HBE cells and transformed HBE lines, BET-1A and BEAS-2B. In addition, fluorescence-activated cell sorter (FACS) analyses using an anti-DDR1 antibody showed that DDR1 was expressed on HBE cells and two HBE lines.
Immunohistochemical staining using human bronchial tissue demonstrated that DDR1 was mainly expressed at the basolateral cell surface of the bronchial epithelium. Furthermore, immunostaining of type IV collagen, a major component of the basement membrane, clearly showed that the basement membrane was closely attached to the basal surface of the bronchial epithelium.
Since collagen binds to and activates discoidin domain receptor 1 tyrosine kinase, colocalization of discoidin domain receptor 1 and its ligand type IV collagen demonstrates a potential interaction of discoidin domain receptor 1 on the bronchial epithelium with type IV collagen. Further study of this interaction may define the functional significance of the collagen-discoidin domain receptor 1 signalling pathway in health and in disease.
This article has been cited by other articles:

|
 |

|
 |
 
Y. Huang, P. Arora, C. A. McCulloch, and W. F. Vogel
The collagen receptor DDR1 regulates cell spreading and motility by associating with myosin IIA
J. Cell Sci.,
May 15, 2009;
122(10):
1637 - 1646.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
U. Rix, O. Hantschel, G. Durnberger, L. L. Remsing Rix, M. Planyavsky, N. V. Fernbach, I. Kaupe, K. L. Bennett, P. Valent, J. Colinge, et al.
Chemical proteomic profiles of the BCR-ABL inhibitors imatinib, nilotinib, and dasatinib reveal novel kinase and nonkinase targets
Blood,
December 1, 2007;
110(12):
4055 - 4063.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
C. Avivi-Green, M. Singal, and W. F. Vogel
Discoidin Domain Receptor 1-deficient Mice Are Resistant to Bleomycin-induced Lung Fibrosis
Am. J. Respir. Crit. Care Med.,
August 15, 2006;
174(4):
420 - 427.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
W. Matsuyama, M. Watanabe, Y. Shirahama, R. Hirano, H. Mitsuyama, I. Higashimoto, M. Osame, and K. Arimura
Suppression of Discoidin Domain Receptor 1 by RNA Interference Attenuates Lung Inflammation
J. Immunol.,
February 1, 2006;
176(3):
1928 - 1936.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
W. Matsuyama, H. Mitsuyama, M. Watanabe, Y. Shirahama, I. Higashimoto, M. Osame, and K. Arimura
Involvement of Discoidin Domain Receptor 1 in the Deterioration of Pulmonary Sarcoidosis
Am. J. Respir. Cell Mol. Biol.,
December 1, 2005;
33(6):
565 - 573.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
W. Matsuyama, M. Watanabe, Y. Shirahama, K.-i. Oonakahara, I. Higashimoto, T. Yoshimura, M. Osame, and K. Arimura
Activation of Discoidin Domain Receptor 1 on CD14-Positive Bronchoalveolar Lavage Fluid Cells Induces Chemokine Production in Idiopathic Pulmonary Fibrosis
J. Immunol.,
May 15, 2005;
174(10):
6490 - 6498.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
R. Abdulhussein, C. McFadden, P. Fuentes-Prior, and W. F. Vogel
Exploring the Collagen-binding Site of the DDR1 Tyrosine Kinase Receptor
J. Biol. Chem.,
July 23, 2004;
279(30):
31462 - 31470.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
C. A. Curat and W. F. Vogel
Discoidin Domain Receptor 1 Controls Growth and Adhesion of Mesangial Cells
J. Am. Soc. Nephrol.,
November 1, 2002;
13(11):
2648 - 2656.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
C. A. Curat, M. Eck, X. Dervillez, and W. F. Vogel
Mapping of Epitopes in Discoidin Domain Receptor 1 Critical for Collagen Binding
J. Biol. Chem.,
November 30, 2001;
276(49):
45952 - 45958.
[Abstract]
[Full Text]
[PDF]
|
 |
|
Copyright © 2001 by the European Respiratory Society.
|