|
 |
|
|||||||
|
|||||||||
Copyright ©ERS Journals Ltd 2001
Surfactant protein A and other bronchoalveolar lavage fluid proteins are altered in cystic fibrosis
1 Children's Clinic of Ludwig-Maximilians-University, Munich, Germany. 2 Children's Clinic, Inselspital, University of Bern, Switzerland
CORRESPONDENCE: M. Griese, Kinderklinik und Kinderpoliklinik im Dr. von Haunerschen Kinderspital, Ludwig-Maximilians University, Pettenkoferstrasse 8a, Munich, Germany, D-80336. Fax: 49 8951603477
Keywords: bronchoalveolar lavage, cystic fibrosis, proteome, surfactant protein A
Received: August 30, 2000
Accepted December 8, 2000
This project was supported by a grant from the Wilhelm Sander Stiftung (Gr 93.002.1/2).
Inflammation and proteolytic processes play an important role in the progression of cystic fibrosis (CF) lung disease. The goal of this study was to describe bronchoalveolar lavage fluid (BALF) protein pattern of CF patients in comparison to controls and to assess if there is proteolytic degradation of surfactant protein A (SP-A), an important innate host defence component of the lungs.
BALFs from 17 clinically stable CF patients and from eight healthy children were separated by two-dimensional gel electrophoresis. Silver staining was used to show BALF proteins and Western blotting to detect SP-A isoforms.
In CF, BALF proteins of a low molecular weight
Proteolytic damage to surfactant protein A and significant changes of normal bronchoalveolar lavage fluid proteins occur in lungs of cystic fibrosis patients. Identification of altered bronchoalveolar lavage fluid proteins may give new insights into pathogenic mechanisms and provide new targets for therapy.
20 kD were more abundant than in controls. Various proteins were seen in CF which were not present in controls and vice versa. Degradation of SP-A was present in 15 of 17 CF BALFs but in none of the controls, in contrast polymeric isoforms were seen in all controls and in four of 17 CF patients.
This article has been cited by other articles:
|
|
 |
|
  C. L. Ventura, R. Higdon, L. Hohmann, D. Martin, E. Kolker, H. D. Liggitt, S. J. Skerrett, and C. E. Rubens Staphylococcus aureus Elicits Marked Alterations in the Airway Proteome during Early Pneumonia Infect. Immun., December 1, 2008; 76(12): 5862 - 5872. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. R. S. Tagaram, G. Wang, T. M. Umstead, A. N. Mikerov, N. J. Thomas, G. R. Graff, J. C. Hess, M. J. Thomassen, M. S. Kavuru, D. S. Phelps, et al. Characterization of a human surfactant protein A1 (SP-A1) gene-specific antibody; SP-A1 content variation among individuals of varying age and pulmonary health Am J Physiol Lung Cell Mol Physiol, May 1, 2007; 292(5): L1052 - L1063. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Starosta, E. Rietschel, K. Paul, U. Baumann, and M. Griese Oxidative Changes of Bronchoalveolar Proteins in Cystic Fibrosis. Chest, February 1, 2006; 129(2): 431 - 437. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. P. Carroll, C. M. Greene, C. C. Taggart, A. G. Bowie, S. J. O'Neill, and N. G. McElvaney Viral Inhibition of IL-1- and Neutrophil Elastase-Induced Inflammatory Responses in Bronchial Epithelial Cells J. Immunol., December 1, 2005; 175(11): 7594 - 7601. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Sloane, R. A. Lindner, S. S. Prasad, L. T. Sebastian, S. K. Pedersen, M. Robinson, P. T. Bye, D. W. Nielson, and J. L. Harry Proteomic Analysis of Sputum from Adults and Children with Cystic Fibrosis and from Control Subjects Am. J. Respir. Crit. Care Med., December 1, 2005; 172(11): 1416 - 1426. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Griese, R. Essl, R. Schmidt, E. Rietschel, F. Ratjen, M. Ballmann, K. Paul, and for the BEAT Study Group Pulmonary Surfactant, Lung Function, and Endobronchial Inflammation in Cystic Fibrosis Am. J. Respir. Crit. Care Med., November 1, 2004; 170(9): 1000 - 1005. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Hirsch, K. C. Hansen, A. L. Burlingame, and M. A. Matthay Proteomics: current techniques and potential applications to lung disease Am J Physiol Lung Cell Mol Physiol, July 1, 2004; 287(1): L1 - L23. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. P. Bowler, B. Duda, E. D. Chan, J. J. Enghild, L. B. Ware, M. A. Matthay, and M. W. Duncan Proteomic analysis of pulmonary edema fluid and plasma in patients with acute lung injury Am J Physiol Lung Cell Mol Physiol, June 1, 2004; 286(6): L1095 - L1104. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F Rubio, J Cooley, F J Accurso, and E Remold-O'Donnell Linkage of neutrophil serine proteases and decreased surfactant protein-A (SP-A) levels in inflammatory lung disease Thorax, April 1, 2004; 59(4): 318 - 323. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Gold, Y. Hoshino, N. Tanaka, W. N. Rom, B. Raju, R. Condos, and M. D. Weiden Surfactant Protein A Modulates the Inflammatory Response in Macrophages during Tuberculosis Infect. Immun., February 1, 2004; 72(2): 645 - 650. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H Clark and K Reid The potential of recombinant surfactant protein D therapy to reduce inflammation in neonatal chronic lung disease, cystic fibrosis, and emphysema Arch. Dis. Child., November 1, 2003; 88(11): 981 - 984. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. L. Noah, P. C. Murphy, J. J. Alink, M. W. Leigh, W. M. Hull, M. T. Stahlman, and J. A. Whitsett Bronchoalveolar Lavage Fluid Surfactant Protein-A and Surfactant Protein-D Are Inversely Related to Inflammation in Early Cystic Fibrosis Am. J. Respir. Crit. Care Med., September 15, 2003; 168(6): 685 - 691. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Sabounchi-Schutt, J. Astrom, U. Hellman, A. Eklund, and J. Grunewald Changes in bronchoalveolar lavage fluid proteins in sarcoidosis: a proteomics approach Eur. Respir. J., March 1, 2003; 21(3): 414 - 420. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Aitken, K. E. Greene, M. R. Tonelli, J. L. Burns, J. C. Emerson, C. H. Goss, and R. L. Gibson Analysis of Sequential Aliquots of Hypertonic Saline Solution-Induced Sputum From Clinically Stable Patients With Cystic Fibrosis Chest, March 1, 2003; 123(3): 792 - 799. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Griese, N. Maderlechner, P. Ahrens, and R. Kitz Surfactant Proteins A and D in Children with Pulmonary Disease due to Gastroesophageal Reflux Am. J. Respir. Crit. Care Med., June 1, 2002; 165(11): 1546 - 1550. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. R. Korfhagen Surfactant Protein A (SP-A)-Mediated Bacterial Clearance . SP-A and Cystic Fibrosis Am. J. Respir. Cell Mol. Biol., December 1, 2001; 25(6): 668 - 672. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |