|
 |
|
|||||||
|
|||||||||
Copyright © ERS Journals Ltd 1999
Clinical Trial |
The effect of processing on inflammatory markers in induced sputum
The effects of the mucolytic agent, dithioerythritol (DTE), and the temperature at which sputum processing is conducted on cellular and biochemical markers in induced sputum was assessed. Samples from healthy and atopic asthmatic subjects were treated with either DTE or phosphate-buffered saline (PBS) at 22 or 37 degrees C and compared for cell counts and concentrations of histamine, tryptase, eosinophil cationic protein (ECP), free interleukin (IL)-8, immunoglobulin (Ig)A, IL-8/IgA complexes and secretory component (SC). In addition, the influence of DTE on in vitro mediator release from blood eosinophils, basophils and bronchoalveolar lavage (BAL) mast cells was studied. Processing with DTE improved cytospin quality and increased the cell yield and measurable ECP, tryptase, IgA and SC, but reduced levels of histamine in PBS-treated samples and had no effect on IL-8. Cell counts or mediator levels were similar when sputum was processed at 22 or 37 degrees C, even though DTE induced blood basophils and BAL mast cells to release histamine at 37 degrees C. In spiking experiments, recovery of added ECP, tryptase, total IL-8 and histamine from sputum was similar in DTE- and PBS-processed sputum, but reduced for free IL-8 in PBS-treated samples. In conclusion, dithioerythritol improves cell and mediator recovery without causing cell activation when sputum processing is conducted at room temperature. The extent of recovery depends on the mediator studied.
This article has been cited by other articles:
|
|
 |
|
  E. M. Erin, G. R. Jenkins, O. M. Kon, A. S. Zacharasiewicz, G. C. Nicholson, H. Neighbour, R. C. Tennant, A. J. Tan, B. R. Leaker, A. Bush, et al. Optimized Dialysis and Protease Inhibition of Sputum Dithiothreitol Supernatants Am. J. Respir. Crit. Care Med., January 15, 2008; 177(2): 132 - 141. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. D. Sagel, J. F. Chmiel, and M. W. Konstan Sputum Biomarkers of Inflammation in Cystic Fibrosis Lung Disease Proceedings of the ATS, August 1, 2007; 4(4): 406 - 417. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Tsoumakidou, E. Papadopouli, N. Tzanakis, and N. M. Siafakas Airway Inflammation and Cellular Stress in Noneosinophilic Atopic Asthma Chest, May 1, 2006; 129(5): 1194 - 1202. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Zacharasiewicz, N. Wilson, C. Lex, E. M. Erin, A. M. Li, T. Hansel, M. Khan, and A. Bush Clinical Use of Noninvasive Measurements of Airway Inflammation in Steroid Reduction in Children Am. J. Respir. Crit. Care Med., May 15, 2005; 171(10): 1077 - 1082. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R A O'Donnell, A Richter, J Ward, G Angco, A Mehta, K Rousseau, D M Swallow, S T Holgate, R Djukanovic, D E Davies, et al. Expression of ErbB receptors and mucins in the airways of long term current smokers Thorax, December 1, 2004; 59(12): 1032 - 1040. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M Delvaux, M Henket, L Lau, P Kange, P Bartsch, R Djukanovic, and R Louis Nebulised salbutamol administered during sputum induction improves bronchoprotection in patients with asthma Thorax, February 1, 2004; 59(2): 111 - 115. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Beeh, O. Kornmann, R. Buhl, S. V. Culpitt, M. A. Giembycz, and P. J. Barnes Neutrophil Chemotactic Activity of Sputum From Patients With COPD: Role of Interleukin 8 and Leukotriene B4 Chest, April 1, 2003; 123(4): 1240 - 1247. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Beeh, J. Beier, O. Kornmann, A. Mander, and R. Buhl Long-term Repeatability of Induced Sputum Cells and Inflammatory Markers in Stable, Moderately Severe COPD Chest, March 1, 2003; 123(3): 778 - 783. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I S Woolhouse, D L Bayley, and R A Stockley Effect of sputum processing with dithiothreitol on the detection of inflammatory mediators in chronic bronchitis and bronchiectasis Thorax, August 1, 2002; 57(8): 667 - 671. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Leader of the Working Group: P.L. Paggiaro, Members of the Working Group:, P. Chanez, O. Holz, P.W. Ind, R. Djukanovic, P. Maestrelli, and P.J. Sterk Sputum induction Eur. Respir. J., July 1, 2002; 20(37_suppl): 3S - 8s. [Full Text] [PDF]
|
|
|
|
|
|
Leader of the Working Group: A. Efthimiadis, A. Sp, Members of the Working Group:, Q. Hamid, M.M. Kelly, M. Linden, R. Louis, M.M.M. Pizzichini, E. Pizzichini, C. Ronchi, F. Van Overveld, et al. Methods of sputum processing for cell counts, immunocytochemistry and in situ hybridisation Eur. Respir. J., July 1, 2002; 20(37_suppl): 19S - 23s. [Full Text] [PDF]
|
|
|
|
|
|
Leader of the Working Group: M.M. Kelly, Members of the Working Group:, V. Keatings, R. Leigh, C. Peterson, J. Shute, P. Venge, and R. Djukanovic Analysis of fluid{-}phase mediators Eur. Respir. J., July 1, 2002; 20(37_suppl): 24S - 39s. [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Wright, P. M. Hockey, G. Enhorning, P. Strong, K. B. M. Reid, S. T. Holgate, R. Djukanovic, and A. D. Postle Altered airway surfactant phospholipid composition and reduced lung function in asthma J Appl Physiol, October 1, 2000; 89(4): 1283 - 1292. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |