ERJ
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Permissions
Right arrowRequest Permissions
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wang, S.
Right arrow Articles by Forsyth, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, S.
Right arrow Articles by Forsyth, K.
Eur Respir J 1998; 12: 612-618
Copyright © ERS Journals Ltd 1998

Original Articles

Neutrophils induce damage to respiratory epithelial cells infected with respiratory syncytial virus

SZ Wang, H Xu, A Wraith, JJ Bowden, JH Alpers, and KD Forsyth

The mechanisms by which respiratory syncytial virus (RSV) infection induces bronchiolitis and airway disease are unclear. The presence of large numbers of polymorphonuclear leukocytes (PMN) in the airways of infants with RSV infection suggests a potential role of PMN in airway injury associated with RSV infection. To investigate the potential role of neutrophils in RSV bronchiolitis, human alveolar type II cells (A549 cells) were infected with different doses of RSV for 6-48 h. A 51Cr-releasing assay was used to measure PMN-induced damage and image analysis was used to determine PMN adhesion and detachment of epithelial cells. The results showed that RSV infection of epithelial cells enhanced PMN adherence in a dose- and time-dependent pattern, RSV infection alone could damage and detach epithelial cells to a limited extent and PMN significantly augmented RSV infection-induced damage and detachment of epithelial cells. These data suggest that respiratory syncytial virus infection of respiratory epithelial cells enhances neutrophil adhesion to the epithelium and that activated neutrophils augment the damage and detachment of epithelium infected with the virus. Polymorphonuclear leukocytes may contribute to the pathogenesis of respiratory syncytial virus airway disease by inducing epithelial damage and cell loss.


This article has been cited by other articles:


Home page
 J. Virol.Home page
M. R. Murawski, G. N. Bowen, A. M. Cerny, L. J. Anderson, L. M. Haynes, R. A. Tripp, E. A. Kurt-Jones, and R. W. Finberg
Respiratory Syncytial Virus Activates Innate Immunity through Toll-Like Receptor 2
J. Virol., February 1, 2009; 83(3): 1492 - 1500.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
T. M. A. Wilkinson, G. C. Donaldson, S. L. Johnston, P. J. M. Openshaw, and J. A. Wedzicha
Respiratory Syncytial Virus, Airway Inflammation, and FEV1 Decline in Patients with Chronic Obstructive Pulmonary Disease
Am. J. Respir. Crit. Care Med., April 15, 2006; 173(8): 871 - 876.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
R. Arnold and W. Konig
Respiratory Syncytial Virus Infection of Human Lung Endothelial Cells Enhances Selectively Intercellular Adhesion Molecule-1 Expression
J. Immunol., June 1, 2005; 174(11): 7359 - 7367.
[Abstract] [Full Text] [PDF]

Home page
 Arch. Dis. Child.Home page
P S McNamara, P Ritson, A Selby, C A Hart, and R L Smyth
Bronchoalveolar lavage cellularity in infants with severe respiratory syncytial virus bronchiolitis
Arch. Dis. Child., October 1, 2003; 88(10): 922 - 926.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S.-Z. Wang, C. L. Rosenberger, Y.-X. Bao, J. M. Stark, and K. S. Harrod
Clara Cell Secretory Protein Modulates Lung Inflammatory and Immune Responses to Respiratory Syncytial Virus Infection
J. Immunol., July 15, 2003; 171(2): 1051 - 1060.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
S. Munir and V. Kapur
Regulation of Host Cell Transcriptional Physiology by the Avian Pneumovirus Provides Key Insights into Host-Pathogen Interactions
J. Virol., April 15, 2003; 77(8): 4899 - 4910.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
A. L. Lambert, F. S. Trasti, J. B. Mangum, and J. I. Everitt
Effect of Preexposure to Ultrafine Carbon Black on Respiratory Syncytial Virus Infection in Mice
Toxicol. Sci., April 1, 2003; 72(2): 331 - 338.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
D. C. Look, M. J. Walter, M. R. Williamson, L. Pang, Y. You, J. N. Sreshta, J. E. Johnson, D. S. Zander, and S. L. Brody
Effects of Paramyxoviral Infection on Airway Epithelial Cell Foxj1 Expression, Ciliogenesis, and Mucociliary Function
Am. J. Pathol., December 1, 2001; 159(6): 2055 - 2069.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S.-Z. Wang, C. L. Rosenberger, T. M. Espindola, E. G. Barrett, Y. Tesfaigzi, D. E. Bice, and K. S. Harrod
CCSP modulates airway dysfunction and host responses in an Ova-challenged mouse model
Am J Physiol Lung Cell Mol Physiol, November 1, 2001; 281(5): L1303 - L1311.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
P.A.B. Wark, S.L. Johnston, I. Moric, J.L. Simpson, M.J. Hensley, and P.G. Gibson
Neutrophil degranulation and cell lysis is associated with clinical severity in virus-induced asthma
Eur. Respir. J., January 1, 2001; 19(1): 68 - 75.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
R. P. Garofalo and H. Haeberle
Epithelial Regulation of Innate Immunity to Respiratory Syncytial Virus
Am. J. Respir. Cell Mol. Biol., November 1, 2000; 23(5): 581 - 585.
[Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1998 by the European Respiratory Society.